A Comparison Between Fibronectin and Matrigel Pretreated ePTFE Vascular Grafts

Patterson, Robert B.; Keller, J. D.; Silberstein, Edward B.; Kempczinski, Richard F.

doi:10.1016/s0890-5096(06)62010-4

Cited by 15 publications

(8 citation statements)

References 25 publications

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“…Their results range from a spontaneous loss of 5% within 60 min in fresh EC from human veins 11 to a loss of 7.8% within the same time period in EC from canine veins. 25 Vhora et al 23 demonstrated that in EC from the human saphenous vein, greatest spontaneous radionuclide loss occurred in the first 30 min after onset of blood flow in the model, becoming nonappreciable over the following 4 h. In the present model spontaneous radionuclide loss was negligible.…”

Section: Discussionsupporting

confidence: 46%

Coating PTFE vascular prostheses with a fibroblastic matrix improves cell retention when subjected to blood flow

Buján

García‐Honduvilla

Contreras

et al. 1998

J. Biomed. Mater. Res.

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An investigation was made into the effect of blood flow on endothelial cells (EC) and mesothelial cells (MC) seeded on a vascular expanded polytetrafluoroethylene (ePTFE) prosthesis coated with a fibroblastic matrix. Endothelial cells were obtained from the external jugular vein and MC from the omentum. To test the performance of prostheses, a custom designed, femoral "ex vivo" circuit was developed in mongrel dogs. Four study groups were established: a control group, A1, where prostheses were uncoated and seeded with EC; a second control group, A2, where prostheses were uncoated and seeded with MC; group B1 where prostheses were coated with a fibroblastic matrix and seeded with EC; and group B2 where coated prostheses were seeded with MC. All cells were labeled with 111Indium oxine (10 microCi/mL) before seeding. After the seeded cells had formed a monolayer on the ePTFE prostheses (which took approximately 24 h) the prostheses were placed in the "ex vivo" circuit. The rates of blood flow to which prostheses were exposed were measured at the point of inflow (117.5 +/- 12.50 mL/min, mean +/- SD) and outflow (72.6 +/- 14.3 mL/min). MC showed a greater baseline radionuclide uptake than did EC. The cells of groups B1 and B2 adhered sufficiently to the fibroblastic matrix and covered enough of the prosthetic surface to be positioned in the "ex vivo" circuit (76.90 +/- 8.24% surface covered in EC-seeded prostheses and 71.65 +/- 6.23% in MC-seeded prostheses). After exposure to blood flow the quantity of radionuclide-labeled cells and the prosthetic surface covered by them were greatly reduced though the fibroblast-coated prostheses showed greater cell retention.

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Section: Discussionsupporting

confidence: 46%

Coating PTFE vascular prostheses with a fibroblastic matrix improves cell retention when subjected to blood flow

Buján

García‐Honduvilla

Contreras

et al. 1998

J. Biomed. Mater. Res.

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“…In addition to fibronectin, vitronectin and matrigel coating also offered relatively good HAEC proliferation, but the initial adhesion of the HAEC was significantly lower compared with the fibronectin coating in our studies. Although the matrix gel consists of various adhesion proteins including fibronectin, studies have shown no significant advantage of using matrigel over fibronectin for surface modification of the scaffold (41). Similar to our results was a study conducted by Cuy et al (42), who found that aortic vascular endothelial cells preferentially adhere to fibronectin, collagen types IV and I over laminin, on the surface modification with adhesive proteins.…”

Section: Discussionmentioning

confidence: 99%

A Combined Strategy To Reduce Restenosis for Vascular Tissue Engineering Applications

Patel

Patterson

et al. 2006

Biotechnol. Prog.

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Biodegradable polymers including poly(l-lactic acid) (PLLA) have been used to develop cardiovascular prostheses such as vascular grafts and stents. However, implant-associated thrombosis, inflammation, and restenosis are still major obstacles for the utility of these devices. The lack of an endothelial cell (EC) lining (endothelialization) on the implants and the responses of the immune systems toward the implants have been associated with these complications. In our research strategy, we have combined the drug delivery principle with the strategies of tissue engineering, the controlled release of anti-inflammation drugs and enhanced endothelialization, to reduce the implant-associated adverse responses. We first integrated curcumin, an anti-inflammatory drug and anti-smooth muscle cell (SMC) proliferative drug, with PLLA. This curcumin-loaded PLLA material was then modified using adsorptive coating of adhesive proteins such as fibronectin, collagen-I, vitronectin, laminin, and matrigel to improve the endothelial cell (EC) adhesion and proliferation, and ECs were seeded on top of these modified surfaces. Our results showed steady drug release kinetics over the period of 50 days from curcumin-loaded PLLA materials. Additionally, integration of curcumin in PLLA increased the roughness of the scaffold at the nanometric scale using an atomic force microscopic analysis. Moreover, coating with fibronectin on curcumin-loaded PLLA surfaces gave the highest EC adhesion and proliferation compared to other adhesive proteins using PicoGreen DNA assays. The ability of our strategy to release the curcumin for producing anti-inflammation and anti-proliferation responses and to improve EC adhesion and growth after EC seeding suggests this strategy may reduce implant-associated adverse responses and be a better approach for vascular tissue engineering applications.

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“…Treatments to improve protein adsorption to graft material include plasma treatment [26,27], chemical treatments [25,26], and fibrin glue [23]. While mixtures of ECM proteins have been employed, such as Matrigel [28], it is more common to employ single integrin-dependent ligands such FN [10] or RGD peptide sequence [29]. However, the low affinity of integrin-dependent binding systems has limited their success.…”

Section: Discussionmentioning

confidence: 99%

Mylar™ and Teflon-AF™ as cell culture substrates for studying endothelial cell adhesion

2005

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A Comparison Between Fibronectin and Matrigel Pretreated ePTFE Vascular Grafts

Cited by 15 publications

References 25 publications

Coating PTFE vascular prostheses with a fibroblastic matrix improves cell retention when subjected to blood flow

Coating PTFE vascular prostheses with a fibroblastic matrix improves cell retention when subjected to blood flow

A Combined Strategy To Reduce Restenosis for Vascular Tissue Engineering Applications

Mylar™ and Teflon-AF™ as cell culture substrates for studying endothelial cell adhesion

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