A Comparison between the cytotoxic effects of pure curcumin and curcumin-loaded PLGA-PEG nanoparticles on the MCF-7 human breast cancer cell line

Mirakabad, Fatemeh Sadat Tabatabaei; Akbarzadeh, Abolfazl; Milani, Morteza; Zarghami, Nosratollah; Taheri‐Anganeh, Mortaza; Zeighamian, Vahideh; Badrzadeh, Fariba; Rahmati-Yamchi, Mohammad

doi:10.3109/21691401.2014.955108

Cited by 95 publications

(35 citation statements)

References 35 publications

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“…The literature shows that the cytotoxic effect of a nanoformulation of curcumin is equal to or higher than that of free curcumin. 38,39 This does not match our results where the cytotoxic effect of CuCs was lower than that of free curcumin. This might be due to i) the lower % of the entrapment efficiency, ii) the nature of the nanocarrier, which is different in the current study than in previous studies, and iii) the antioxidant properties of CsNPs as previously reported by Nair et al 40 Further extensive investigations using simulated CompuSyn software (Linear Interaction Effect) were conducted to investigate possible additivity between curcumin and CsNPs, which might predict the synergistic effect of CuCs nanocomposite as antiviral activity.…”

Section: Discussioncontrasting

confidence: 99%

<p>Antiviral Activity of Chitosan Nanoparticles Encapsulating Curcumin Against Hepatitis C Virus Genotype 4a in Human Hepatoma Cell Lines</p>

Loutfy¹,

Elberry²,

Farroh³

et al. 2020

IJN

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Purpose: Current direct-acting antiviral agents for treatment of hepatitis C virus genotype 4a (HCV-4a) have been reported to cause adverse effects, and therefore less toxic antivirals are needed. This study investigated the role of curcumin chitosan (CuCs) nanocomposite as a potential anti-HCV-4a agent in human hepatoma cells Huh7. Methods: Docking of curcumin and CuCs nanocomposite and binding energy calculations were carried out. Chitosan nanoparticles (CsNPs) and CuCs nanocomposite were prepared with an ionic gelation method and characterized with TEM, zeta size and potential, and HPLC to calculate encapsulation efficiency. Cytotoxicity studies were performed on Huh7 cells using MTT assay and confirmed with cellular and molecular assays. Anti-HCV-4a activity was determined using real-time PCR and Western blot. Results: The strength of binding interactions between protein ligand complexes gave scores with NS3 protease, NS5A polymerase, and NS5B polymerase of -124.91, -159.02, and -129.16, for curcumin respectively, and -68.51, -54.52, and -157.63 for CuCs nanocomposite, respectively. CuCs nanocomposite was prepared at sizes 29-39.5 nm and charges of 33 mV. HPLC detected 4% of curcumin encapsulated into CsNPs. IC50 was 8 µg/mL for curcumin and 25 µg/ mL for the nanocomposite on Huh7 but was 25.8 µg/mL and 34 µg/mL on WISH cells. CsNPs had no cytotoxic effect on tested cell lines. Apoptotic genes' expression revealed the caspasedependent pathway mechanism. CsNPs and CuCs nanocomposite demonstrated 100% inhibition of viral entry and replication, which was confirmed with HCV core protein expression. Conclusion: CuCs nanocomposite inhibited HCV-4a entry and replication compared to curcumin alone, suggesting its potential role as an effective therapeutic agent. Plain Language SummaryHepatitis C virus genotype 4a (HCV-4a) is a major public health problem, especially among the Egyptian population. There are more than 70 million individuals infected worldwide. It is the leading cause of chronic liver diseases, cirrhosis, and hepatocellular carcinoma. Newly emerged anti-HCV viral agents have recently become available on the market but with serious complications, high cost, and possible development of resistance. This has encouraged scientists to search for alternative, safer antiviral approaches. Nanoparticles offer unique physical properties that have associated benefits for antimicrobial activity or as drug carrier that can improve antiviral therapy. The significance of our research is in establishment of a natural nanoparticle drug carrier system that we screened with computer simulation studies and in cells against HCV-4a replication into human hepatoblastoma cell

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Section: Discussioncontrasting

confidence: 99%

<p>Antiviral Activity of Chitosan Nanoparticles Encapsulating Curcumin Against Hepatitis C Virus Genotype 4a in Human Hepatoma Cell Lines</p>

Loutfy¹,

Elberry²,

Farroh³

et al. 2020

IJN

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“…One major advantage of lipid vesicles is its ability to fuse with cellular membranes or membranes of intracellular organelles. This allows for much versatility in their ability to deliver drugs to different sites of the cells (Abbasi et al 2016a, Abbasi et al 2016b, Alizadeh et al 2016, Chang 1966, Chung et al 2016, Daraee et al 2016a, Daraee et al 20146b, Davoudi et al 2014, Eatemadi et al 2016, Ebrahimi et al 2016, Fekri Aval et al 2016, Hosseininasab et al 2014, Nasrabadi et al 2016, Sadat Tabatabaei Mirakabad et al 2016.…”

Section: Drug Delivery Based On Mnpsmentioning

confidence: 99%

Magnetic nanoparticles: preparation methods, applications in cancer diagnosis and cancer therapy

Khiabani

Farshbaf

Akbarzadeh

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

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101

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Super-paramagnetic iron oxide nanoparticles (SPIONs) have been widely explored as novel magnetic resonance imaging (MRI) contrast agents because of a combination of favorable super-paramagnetic properties, biodegradability, and surface properties. Among the numerous MNPs under examination, SPIONs have involved considerable interest due to their outstanding magnetic properties, biocompatibility, and biodegradability. This review debates the ABCs of MR imaging, reports and informs the state of the art of magnetic nanoparticles in cancer dealing the source of SPIONs' exclusive magnetic properties, recent progresses in MRI acquisition methods for detection of SPIONs.

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“…Absorbance of the formazan solution was measured at 570 nm (650 nm background) on a Spectra Max plate reader. The results were expressed as mean  standard deviation of 3 measurements (Tabatabaei Mirakabad et al 2015, Luo et al 2014). …”

Section: In Vitro Cytotoxicity Studymentioning

confidence: 99%

In vitro and in vivo evaluation of PEGylated nanoparticles of bendamustine for treatment of lung cancer

Dubey

Gidwani

Pandey

et al. 2015

Artificial Cells, Nanomedicine, and Biotechnology

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The purpose of this study was to develop PEGylated nanoparticles of bendamustine (BM) to improve therapeutic efficiency of drug and reduce the side-effects. The nanoparticles were prepared by a modified diffusion-emulsification method. The particle size and zeta potential of optimized BM-loaded PEGylated NPs were found to be 256 nm and -29.1 mV. The in vitro release showed biphasic behavior, with initial burst release followed by slow sustained delivery. The anti-tumor activity was determined using the A- 549 cell line, by the MTT assay. The stability study revealed that the nanoparticles prepared were stable for 3 months at both 25°C and 4°C.

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A Comparison between the cytotoxic effects of pure curcumin and curcumin-loaded PLGA-PEG nanoparticles on the MCF-7 human breast cancer cell line

Cited by 95 publications

References 35 publications

<p>Antiviral Activity of Chitosan Nanoparticles Encapsulating Curcumin Against Hepatitis C Virus Genotype 4a in Human Hepatoma Cell Lines</p>

<p>Antiviral Activity of Chitosan Nanoparticles Encapsulating Curcumin Against Hepatitis C Virus Genotype 4a in Human Hepatoma Cell Lines</p>

Magnetic nanoparticles: preparation methods, applications in cancer diagnosis and cancer therapy

In vitro and in vivo evaluation of PEGylated nanoparticles of bendamustine for treatment of lung cancer

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