A Comparison of 35s Methionine Incorporation by the Blastocysts of Normal and Delayed Implanting Mice

Weitlauf, Harry M.; Greenwald, Gilbert S.

doi:10.1530/jrf.0.0100203

Cited by 46 publications

(14 citation statements)

References 9 publications

(10 reference statements)

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“…The delayed implanting eggs had approximately 1/4 the total activity of the normal eggs and 1/10 the incorporated radioactivity. This result in vitro agreed with our earlier results using 35S methionine in vivo (18,19,23,26 Portions of this document may be illegible in electronic image products. Images are produced from the best available original document.…”

Section: Incorporation Of Amino Acids By Normal and Delayedsupporting

confidence: 91%

PROTEIN SYNTHESIS IN DELAYED IMPLANTING MOUSE OVA. Technical Progress Report, May 1, 1971--May 1, 1972.

Weitlauf¹

1972

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This report covers work completed and in progress under AEC *\Eontract number AT(11-1)-1801 during the period of May 1, 1971 -May 1, 1972. The specific areas of experimentation include:(1) incorporation of amino acids by normal and delayed implanting blastocysts in vitro; (2) effect of ovarian hormones on incorporation of amino acids by normal and delayed implanting mouse blastocysts in vitr6; (3) effect of uterine extract on protein synthesis by mouse blastocysts in vitro; (4) chemical measurement of protein in mouse blastocysts; (5) metabolic rate in normal and delayed implanting mouse blastocysts; and (6) the effect of progesterone on the dry weight and lipid content of delayed implanting mouse embryos. The specific experiments were contained in Section B (paragraphs 1 a,b,c; and 2b) of the renewal proposal submitted for 1971-1972.

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Section: Incorporation Of Amino Acids By Normal and Delayedsupporting

confidence: 91%

PROTEIN SYNTHESIS IN DELAYED IMPLANTING MOUSE OVA. Technical Progress Report, May 1, 1971--May 1, 1972.

Weitlauf¹

1972

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“…Their uptake and incorporation into the mouse embryo has been demonstrated both in vitro (Brinster 1971;Smith and Smith 1971;Epstein and Smith 1973;Epstein 1975) and in vivo (Greenwald and Everett 1959;Weitlauf and Greenwald 1965) and some have been shown to be important for the outgrowth of the embryo in vitro (Gwatkin 1966). The present findings indicate that amino acids influence the metabolism of glucose by the embryo by limiting the entry of glucose carbon into non-glycogen macromolecules and directing more label into the synthesis of acid-soluble glycogen.…”

Section: Discussionsupporting

confidence: 50%

Influence of Environmental Factors on the Metabolism of Glucose by Preimplantation Mouse Embryos in vitro

Edirisinghe¹,

Wales²

1985

Aust. Jnl. Of Bio. Sci.

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The metabolism of glucose by late pre implantation mouse embryos was studied in a variety of media whose composition had been changed to reflect the environmental conditions in the uterus more closely than do standard culture media. The effects of combinations of energy substrates, the presence or absence of amino acids and the level of potassium in the medium were investigated.The use of energy substrates for in vitro culture at levels present in the uterine environment resulted in rates of synthesis and degradation of glycogen pools similar to those obtained using standard in vitro culture conditions but elevated incorporation into non-glycogen macromolecules. Amino acids influenced the metabolism of glucose by limiting the entry of glucose carbon into the non-glycogen macromolecular pool and directing more glucose into the synthesis of acid-soluble glycogen.Increasing the K + concentration to 60 mM in the culture medium caused a small but significant increase in the number of eight-cell embryos degenerating during culture for 24 h but the metabolism of glucose was unaffected over this time. At the time of morula transformation to the blastocyst this level of potassium ions suppressed glycogen synthesis by 50% over 5 h but did not affect its turnover during chase culture.It is concluded that factors other than those studied here contribute to the maintenance of the low glycogen levels found in uterine embryos. IntrodnctionEarlier work (Ozias and Stern 1973;Ozias and Weitlauf 1971;Snyder et al. 1971;Edirisinghe et al. 1984aEdirisinghe et al. , 1984b indicates that the uterine environment influences the metabolism of late preimplantation mouse embryos and in particular the utilization of glycogen stores. This environment is more complex than, and differs in the range and concentration of components from, the standard chemically defined media normally used for in vitro culture (Hamner 1971;Wales 1973;Beier 1974; Edirisinghe and Wales 1985). Changes in the environment may well be important in modifying the metabolism of glucose by embryos and be responsible for the differences in the turnover of glycogen in vivo as compared to its utilization in vitro.The possible control of glycogen metabolism by the level of exogenous energy substrate has been studied using different concentrations of the major energy substrates, glucose, lactate and pyruvate (Pike and Wales 1982). It is clear from this study that embryos, especially at the late preimplantation stages, have the ability to synthesize large amounts of glycogen and that the presence of the alternative energy substrates, lactate and pyruvate during culture influences the synthesis of glycogen, particularly when the medium contains a low concentration of glucose. Recently, the levels of lactate, pyruvate and glucose in the uterine fluid of mice have been measured and found 0004-9417/85/040411 $02.00

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“…Weitlauf & Greenwald (1965) using the lactational delay of implantation in mice showed an enhanced incorporation of 35S-methionine, indicative of protein synthesis, 24 hr after the removal of the young in a manner comparable to the condition on the 5th day of normal implantation. However, some of the other workers have encountered difficulties in in-vivo labelling pattern in the pre-implantation stages of blastocysts which may be due to (a) dilution of the precursors by the pre-existing pools in the various tissues of the animal, (b) competitive utiliza¬ tion of the precursors by tissues other than the target sites, i.e.…”

Section: Introductionmentioning

confidence: 92%

Action of Oestrogen on the Blastocyst and Uterus in Delayed Implantation-- An Autoradiographic Study

Prasad¹,

Dass²,

Mohla³

1968

Reproduction

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A Comparison of 35s Methionine Incorporation by the Blastocysts of Normal and Delayed Implanting Mice

Cited by 46 publications

References 9 publications

PROTEIN SYNTHESIS IN DELAYED IMPLANTING MOUSE OVA. Technical Progress Report, May 1, 1971--May 1, 1972.

PROTEIN SYNTHESIS IN DELAYED IMPLANTING MOUSE OVA. Technical Progress Report, May 1, 1971--May 1, 1972.

Influence of Environmental Factors on the Metabolism of Glucose by Preimplantation Mouse Embryos in vitro

Action of Oestrogen on the Blastocyst and Uterus in Delayed Implantation-- An Autoradiographic Study

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