A comparison of a new campylobacter selective medium (CAT) with membrane filtration for the isolation of thermophilic campylobacters including Campylobacter upsaliensis

Aspinall, S T; Wareing, D. R. A.; Hayward, Peter G.; Hutchinson, D. N.

doi:10.1111/j.1365-2672.1996.tb03269.x

Cited by 50 publications

(29 citation statements)

References 22 publications

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“…Whether all C. upsaliensis isolates demonstrate this level of cefoperazone resistance is unknown. However, data from this and other studies (2,3) suggest that the sensitivity of the type strain to cefoperazone is the exception rather than the rule. Therefore, it would appear that factors other than, or in addition to, cefoperazone concentration underlie the superiority of CAT medium over mCCDA for isolating C. upsaliensis.…”

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confidence: 62%

“…Using a modification of blood-free selective media containing reduced levels of cefoperazone, Aspinall et al (2,3) demonstrated recovery of C. upsaliensis on cefoperazone amphotericin teicoplanin selective medium (CAT) that was equivalent to recovery using membrane filtration and considerably superior to recovery on mCCDA. Subsequently, CAT was shown to be superior to mCCDA for the isolation of C. upsaliensis using a semiquantitative plating method (6).…”

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confidence: 99%

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Basis of the Superiority of Cefoperazone Amphotericin Teicoplanin for Isolating Campylobacter upsaliensis from Stools

et al. 2001

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The optimum method for isolating Campylobacter upsaliensis from stools has not been clearly defined. In a preliminary study, cefoperazone amphotericin teicoplanin (CAT) selective medium isolated six C. upsaliensis strains which were not detected using modified cefoperazone charcoal deoxycholate (mCCDA). In order to identify the factors that underlie the superiority of CAT over mCCDA for isolating C. upsaliensis, we examined the effect of incubation time and antibiotic content of culture media on the growth of C. upsaliensis isolates using semiquantitative methods. The recovery of a subgroup of C. upsaliensis isolates from seeded stool specimens was also evaluated. Differences in growth of C. upsaliensis on CAT and mCCDA were modest and were not explained by the antibiotic profiles of the two media. Recovery of C. upsaliensis from spiked human feces on CAT was superior to that on mCCDA at lower concentrations of organisms (10 3 CFU/ml). We conclude that although CAT is more suitable than mCCDA for the isolation of C. upsaliensis from stools, the superiority of CAT for detecting this organism is not accounted for by the antibiotic composition of the medium.

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confidence: 62%

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confidence: 99%

Basis of the Superiority of Cefoperazone Amphotericin Teicoplanin for Isolating Campylobacter upsaliensis from Stools

et al. 2001

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“…Modified selective medium containing cefoperazone at lower concentrations may better support the growth of non-C jejuni/ C coli campylobacter. 7 A cost effective alternative to PCR might be a combination of two media, as is currently used for the detection of salmonellae. For epidemiological purposes, all culture positive samples could then be tested by PCR to identify the isolates to the species level.…”

Section: Discussionmentioning

confidence: 99%

Detection of campylobacter species: a comparison of culture and polymerase chain reaction based methods

Kulkarni

2002

Journal of Clinical Pathology

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Aims: To investigate the optimal method for the detection of campylobacters from stool samples by comparing selective culture with membrane filtration and the polymerase chain reaction (PCR). Methods: Three hundred and forty three stool samples were investigated by each of the three methods mentioned above. Selective culture was performed with charcoal cefoperazone desoxycholate agar plates. Membrane filtration was performed using cellulose triacetate membranes with 0.45 µm pores placed on blood agar plates. Enteropathogenic campylobacters were detected using a PCR identification algorithm, consisting of screening PCRs and species identification using a PCR enzyme linked immunosorbent assay (PCR-ELISA), both based on the 16S rRNA gene. Results: Of the 343 samples tested, 23 were positive by one or more method. Of these, 17 were positive by selective culture, 12 by membrane filtration, and 20 by the PCR identification algorithm. A total of 18 of 23 positives were identified as C jejuni and/or C coli by the PCR identification algorithm, compared with 14 identified to the genus level by selective culture, and 10 by membrane filtration. Among the remaining five positive samples, one C hyointestinalis was detected only by the PCR identification algorithm; one C upsaliensis was detected only by the PCR identification algorithm; one Campylobacter sp was detected by membrane filtration and selective culture and later identified as C concisus; one Campylobacter sp was detected by membrane filtration alone and later identified as Arcobacter sp; and one Campylobacter sp detected only by selective culture was lost to study and therefore not speciated. There was no significant difference between detection by selective culture and the other two methods. However, detection by PCR was significantly better than by membrane filtration (0.05 > p > 0.02). Conclusion: The PCR identification algorithm can detect and identify Campylobacter spp to the species level and the result is obtained on the same day. However, PCR is expensive, labour intensive, and does not provide an isolate for further identification or typing. Selective culture is as good as the PCR identification algorithm for the detection of the two most common species, C jejuni and C coli, and it is cheap and practical. However, it does miss the less common species, results take 48 hours, and identification is only to the genus level. Membrane filtration showed a low sensitivity compared with the other methods and is not appropriate for the diagnostic laboratory, although it was the only method to detect the Arcobacter sp. The optimum method for the detection of campylobacters from stool samples in the diagnostic laboratory remains selective culture.

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“…Membrane filtration method has been extensively used for the isolation of Campylobacter spp. (non selective agar base e.g., blood agar base, Mueller Hinton agar, Brucella agar supplemented with 5-7% defibrinated sheep blood) [26][27][28] . Although faecal-based methods are still the most widely used and considered to be reliable detection methods for Campylobacter in animals, their detection ranges are variable with each procedure [9,[26][27][28][29] .…”

Section: Discussionmentioning

confidence: 99%

“…(non selective agar base e.g., blood agar base, Mueller Hinton agar, Brucella agar supplemented with 5-7% defibrinated sheep blood) [26][27][28] . Although faecal-based methods are still the most widely used and considered to be reliable detection methods for Campylobacter in animals, their detection ranges are variable with each procedure [9,[26][27][28][29] . Thermophilic Campylobacter spp., C. jejuni, C. coli, C. lari and C. upsaliensis are commensally present in the intestinal flora of dogs and cats [30,31] .…”

Section: Discussionmentioning

confidence: 99%

Recovery of Thermophilic Campylobacter spp. in Healthy and Diarrhoeic Pets by Three Culture Methods and Identification of the Isolates by Multiplex Polymerase Chain Reaction (mPCR)

Abay¹,

Aydın²,

Hızlısoy³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryIn this study, the determination of prevalence of thermophilic Campylobacter species in dogs and cats with and without diarrhoea using 3 different cultural methods was aimed. For this purpose, rectal swabs were collected from 120 dogs and 15 cats and 14 of them (12 dogs and 2 cats) were taken from diarrhoeic animals. The isolation of thermophilic Campylobacter spp. was conducted by direct plating onto modified charcoal cefoperazone deoxycholate agar (mCCDA) supplemented with CCDA (cefoperazone, amphotericin B) or CAT (cefoperazone, amphotericin and teicoplanin) for all samples and membrane filtration method onto Mueller-Hinton Agar supplemented with 5% defibrinated sheep blood for samples from diarrhoeic pets and identification of isolates was performed using multiplex polymerase chain reaction (mPCR). The overall prevalence of Campylobacter species was found to be 40.0% and 26.7% in dogs and cats, respectively. Campylobacter jejuni was the most frequent bacterium isolated from 36 dogs and 4 cats. C. upsaliensis, C. coli and C. lari were isolated from 10 dogs and 1 cat, 5 dogs and 2 healthy dogs respectively. For the isolation of thermophilic Campylobacter spp., whilst the method using CAT as selective supplement being more sensitive in dogs, the membrane filtration appeared as the most suitable method in diarrhoeic dogs. These results showed the occurrence of a relatively high carriage of Campylobacter spp., particularly in healthy dogs that may constitute a non negligible risk for public health. Keywords: Campylobacter spp., Membrane filtration method, mPCR, Pet animals, Rectal swab Sağlıklı ve İshalli Pet Hayvanlarda Termofilik Campylobacter spp.'nin Üç Kültür Metodu ile İzolasyonu ve İzolatların Multipleks Polimeraz Zincir Reaksiyonu m(PZR) İle İdentifikasyonu ÖzetBu çalışmada, sağlıklı ve ishalli kedi ve köpeklerde 3 farklı kültür metodu kullanılarak termofilik Campylobacter türlerinin prevalansının belirlenmesi amaçlandı. Bu amaçla, 120 köpek, 15 kediden rektal svap örneği toplandı ve bunların 14'ü (12 köpek ve 2 kedi) ishalli hayvanlardan alındı. Termofilik Campylobacter spp. 'nin izolasyonunda, tüm örnekler için CCDA (cefoperazone, amphotericin B) ya da CAT suplement (cefoperazone, amphotericin and teicoplanin) ilave edilmiş modified charcoal cefoperazone deoxycholate agar (mCCDA)'a (sefoperazon, amfoterisin B) direkt ekim, ishalli hayvanlardan alınan örnekler için de %5 defibrine koyun kanı ilave edilmiş Mueller-Hinton Agar üzerine membran filtrasyon yöntemi kullanıldı. İzolatların identifikasyonu multipleks polimeraz zincir reaksiyonu (mPZR) ile gerçekleştirildi. Campylobacter türlerinin köpek ve kedilerde genel prevalansı sırasıyla %40.0 ve %26.7 olarak bulundu. Campylobacter jejuni en sık rastlanan tür olup 36 köpek ve 4 kediden izole edildi. Ayrıca, 10 köpek ve 1 kediden C. upsaliensis, 5 köpekten C. coli ve 2 sağlıklı köpekten C. lari izole edildi. Termofilik Campylobacter spp. izolasyonu için CAT selektif suplementin kullanıldığı metot köpeklerde daha duyarlı iken ishalli köpeklerde membran...

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A comparison of a new campylobacter selective medium (CAT) with membrane filtration for the isolation of thermophilic campylobacters including Campylobacter upsaliensis

Cited by 50 publications

References 22 publications

Basis of the Superiority of Cefoperazone Amphotericin Teicoplanin for Isolating Campylobacter upsaliensis from Stools

Basis of the Superiority of Cefoperazone Amphotericin Teicoplanin for Isolating Campylobacter upsaliensis from Stools

Detection of campylobacter species: a comparison of culture and polymerase chain reaction based methods

Recovery of Thermophilic Campylobacter spp. in Healthy and Diarrhoeic Pets by Three Culture Methods and Identification of the Isolates by Multiplex Polymerase Chain Reaction (mPCR)

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