A comparison of aberration distribution and cell-cycle progression in cells treated with bleomycin with those exposed to X-rays

Chatterjee, Anupam; Raman, Mercy J.

doi:10.1016/0027-5107(88)90162-5

Cited by 17 publications

(5 citation statements)

References 15 publications

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“…In accord with other studies on the biomonitoring of cytogenic changes we used peripheral blood lymphocytes (Brewen and Genozian 1971;Evans 1982;Rigand et al 1990;Ramesh and Bhargava 1992). For treating the lymphocytes we chose bleomycin, an agent with a well-defined clastogenic effect (Chatterjee and Raman 1988). A further reason to select bleomycin was the idea that its radiomimetic action enables better simulation of the environmental conditions, and particularly the impact of X-irradiation on the cellular genome.…”

Section: Discussionmentioning

confidence: 99%

Latent chromosomal instability in cancer patients

Tzancheva

Komitowski

1996

Hum Genet

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There is increasing evidence that, similar to what is found with other genetic disorders, genomic instability is one of the most general features of cancer. Different forms of manifestation including latent instability have been suggested. To recognize latent chromosomal instability we treated lymphocyte cultures of cancer patients and healthy persons with caffeine, two different doses of bleomycin, and a combination of bleomycin and caffeine. The preliminary results demonstrate that, although the rate of spontaneous chromosomal aberrations is similar in both investigated groups, the lymphocytes of cancer patients display an increased susceptibility to treatment with bleomycin and caffeine. In distinguishing between healthy individuals and those with malignancy, treatment with 30 micrograms/ml bleomycin appears to be most important. Values of chromosomal change above one break per cell, more than 45% cells with chromosomal alterations, and more that two cells with chromosomal rearrangements are suggestive of malignancy. These findings imply that treatment of lymphocyte cultures with bleomycin and caffeine could be a useful assay for monitoring chromosomal instability, and thus detecting a predisposition to malignant disease. In this respect further investigations on a greater amount of material should be performed.

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Section: Discussionmentioning

confidence: 99%

Latent chromosomal instability in cancer patients

Tzancheva

Komitowski

1996

Hum Genet

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“…Possibly the strand breaks produced by APH were the reason for the observed effect . Irradiation with Xrays or UV light is known to delay the cell cycle progression in many cells but Chatterjee & Raman (1988) found that exposure of lymphocytes to BLM did not result in such a delay and suggested that BLM was only causing DNA strand breaks and no other cellular damage . Therefore it is unlikely that the cell cycle delay after irradiation is responsible for improved transformation rates .…”

Section: Resultsmentioning

confidence: 99%

Studies of the mechanism of transgene integration into plant protoplasts: improvement of the transformation rate

1995

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The production of transgenic plants by means of direct gene transfer to protoplasts is now a widely-used technique . The biological mechanisms underlying the transformation are still poorly understood, but many investigations have attempted to shed light on some components of this process . Varying the experimental conditions has in some cases led to better transformation rates, but further improvements of the protocols are possible . Such improvements will require a better understanding of how the alien DNA enters the cells, becomes integrated into the chromosomes and is treated as a part of the plant genome . Irradiation with sublethal doses of X-rays or UV-light has been shown to increase the transformation frequency, while certain drugs have been shown to act in a similar manner . The effects of these and other factors are discussed .Abbreviations: Aph-aphidicolin,ATF-absolutetransformationfrequency,BLM-bleomycin,CaMV-cauliflower mosaic virus, CAT -chloramphenicol acetyl transferase, CHO -Chinese hamster ovary cells, EF -enhancement factor, Nos -nopaline synthase, NPTII -neomycin phosphotransferase II, Ocs -octopine synthase, PEGpolyethyleneglycol, RTF -relative transformation frequency

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“…1978) and the cellular target for CAs induction by BLM is DNA (Evans, 1977). At equal levels of deletions and rearrangements induced by 40 (ig/ml BLM and 2 Gy X-rays in muntjac lymphocytes the frequency of aberrant metaphases and the delay in cell cycle kinetics are less in BLM-treated cultures than in the irradiated one (Chatterjee and Jacob-Raman, 1988). As outlined in the Introduction, this study was carried out to check the effect of endogenous GSH depletion by BSO on the DNA-damaging ability of BLM in normal cells.…”

Section: Discussionmentioning

confidence: 99%

“…Bleomycin (BLM) is a glycopeptide antibiotic, anti-tumour drug that has been shown to produce chromosome aberrations (CAs) comparable with those induced by X-irradiation (Promchainant, 1975;Scott and Zampetti-Bosseler, 1985;Chatterjee and Jacob-Raman, 1988) and it induces CAs at all stages of the cell cycle (Povirk and Austin, 1991). As in the case of indirect action of radiation, BLM is also known to induce DNA breaks through the production of free radicals (Sausville etai, 1976;Lown and Sim, 1977;Takeshita etai, 1978).…”

Section: Introductionmentioning

confidence: 99%

Modulation of the clastogenic activity of bleomycin by reducedglutathione, glutathione-ester and buthionine sulphoximine

1997

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In this study an attempt has been made to establish a relationship between bleomycin (BLM)-induced DNA damage and buthionine sulphoximine (BSO)-mediated modified endogenous glutathione (GSH) status in normal human lymphocytes. Present results demonstrate that depletion of endogenous GSH by BSO reduced the clastogenic action of BLM, whereas elevation of endogenous GSH by treating the cells with GSH and GSH-ester, potentiates the cytotoxicity of BLM. A significant reduction in the frequency of deletions and chromatid breaks was observed when BSO-treated cells were treated with BLM. Again the frequency of these two types of aberrations was increased significantly when GSH- and GSH-ester-treated cells were treated with BLM. The observed reduction in the effect of BLM in GSH-depleted cells could be explained on the basis of the failure of reactivation of the oxidized BLM by reducing agent GSH which is present endogenously. Similarly, it appears that radicals which are generated due to reduction of oxidized BLM by the increased level of cellular GSH, after treating the cells with GSH or GSH-ester, could be responsible for the increasing frequency of deletion and chromatid breaks.

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A comparison of aberration distribution and cell-cycle progression in cells treated with bleomycin with those exposed to X-rays

Cited by 17 publications

References 15 publications

Latent chromosomal instability in cancer patients

Latent chromosomal instability in cancer patients

Studies of the mechanism of transgene integration into plant protoplasts: improvement of the transformation rate

Modulation of the clastogenic activity of bleomycin by reducedglutathione, glutathione-ester and buthionine sulphoximine

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