A Comparison of Antinociceptive Activity of Mycelial Extract from Three Species of Fungi of Basidiomycetes

Abstract

Abstract:The antinociceptive activity of mycelial extract from fermented mushroom of Ganoderma lucidum Coprinus comatus and Grifola frondosa were studied in this paper. The writhing test, formalin test and hot-plate test were used to evaluate the antinociceptive activity of the acetone extracts of the three Species of Fungi of Basidiomycetes in the study respectively. The extract of Ganoderma lucidum inhibited in a dose-dependent manner the acetic acid-induced abdominal constrictions in mice (p<0.05). Also both phases of the response were significantly inhibited in the formalin test (p<0.05). In the hot-plate test, results showed that extract of Ganoderma lucidum significantly inhibited the reaction time to thermal stimuli at 30, 60, and 90 min (p<0.05). The preliminary toxicologic study demonstrated the safety of it. However the extract from fermented mushroom of Coprinus comatus and Grifola frondosa did not show significantly expectative antinociceptive effect. Our results show the acetone extract of Ganoderma lucidum is a potent analgesic drug.

Pharmacological and Pharmacokinetic Studies with Agaricoglycerides, Extracted from Grifola frondosa, in Animal Models of Pain and Inflammation

“…It accounts for the anti-inflammatory and antinociceptive activity of AGF. It is consistent with our previous report that the crude extract containing a mixture of agaricoglycerides from G. frondosa had no beneficial effect at lower dose in mice subjected to acetic acid writhing test, formalin test, and a hot plate test [27]. In summary, the present study demonstrated that AGF at the dose level of 500 mg/kg has important antiinflammatory and antinociceptive effects in preclinical models of inflammation and in some models of pain and thus may be used as an alternative medicine for inflammatory pain.…”

“…Inducible NOS is induced in response to inflammatory-like stimuli and is capable of sustained production of high levels of NO that predominate during inflammation [26]. The excessive or inappropriate production of NO can damage tissue through the superoxide anion (O 2 − ) [27]. The iNOS protein expressions decreased in AGF (500)-treated groups.…”

Mass Spectrometry-Based Untargeted Metabolomics and α-Glucosidase Inhibitory Activity of Lingzhi (Ganoderma lingzhi) During the Developmental Stages

“…Ganoderic acid A ( 50 ), ganoderic acid B ( 43 ), 41 , and 49 isolated from fruiting bodies of G. lingzhi were reported as anti-nociceptive components [46]. Moreover, the strong effect of acetone extract of G. lingzhi mycelium on anti-nociceptive activity has also been reported [47]. These facts show that there is a strong correlation between secondary metabolites contained in crude extracts of natural products and their biological activity.…”

Isolation and biological activity of triglycerides of the fermented mushroom of Coprinus Comatus

“…The agaricoglycerides are a new class of fungal secondary metabolites that constitute esters of chlorinated 4-hydroxy benzoic acid and glycerol. Although the crude extract from Coprinus comatus was no beneficial effect at lower doses in mice subjected to acetic-acid writhing test, formalin test and hot-plate test [ 6 ], there has been no report on the antinociceptive activity of agaricoglycerides extract from Coprinus comatus. Therefore, the objectives of the present study were to demonstrate the production, isolation, and biological properties of triglycerides of the fermented mushroom of Coprinus comatus.…”