A comparison of autologous and allogenic bone marrow-derived mesenchymal stem cell transplantation in canine spinal cord injury

Jung, Dong‐In; Ha, Jung Min; Kang, Byeong‐Teck; Kim, Juwon; Quan, Fu‐Shi; Lee, Jong‐Hwan; Woo, Eun Jin; Park, Hee‐Myung

doi:10.1016/j.jns.2009.05.027

Cited by 152 publications

(148 citation statements)

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“…The transplantation not only of autologous but also of allogenic sources could provide benefits in comparison to other common procedures in bone regeneration. As MSCs have low immune characteristics, they appear to be suitable for allogenic therapeutic purposes, without activating the immune response in immunocompetent patients (Jung et al, 2009). In different studies the use of MSCs has been investigated to replace lost or damaged bone (Schaefer et al, 2000;Ringe et al, 2002).…”

Section: Stem Cells For Bone Regenerationmentioning

confidence: 99%

Oral Tissues as Source for Bone Regeneration in Dental Implantology

Khan¹,

Kleinfeld²,

Winter³

et al. 2012

Tissue Regeneration - From Basic Biology to Clinical Application

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Section: Stem Cells For Bone Regenerationmentioning

confidence: 99%

Oral Tissues as Source for Bone Regeneration in Dental Implantology

Khan¹,

Kleinfeld²,

Winter³

et al. 2012

Tissue Regeneration - From Basic Biology to Clinical Application

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“…[2][3][4] The intrathecal technique of cell injection has also been used in other animal models. [5][6][7] However, the phenotype of LP transplanted neural stem/progenitor cells (NSPCs) and bone marrow-derived mesenchymal stromal cells (BMSCs) and their spatial distribution along the intact and injured spinal cord has not been reported or quantitated. LP is a minimally invasive method of cell delivery, and stem cells may be well suited for LP transplantation because of their responsiveness to signals from the injured CNS.…”

Section: Introductionmentioning

confidence: 99%

Intrathecal transplantation of stem cells by lumbar puncture for thoracic spinal cord injury in the rat

et al. 2011

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Study design: Experimental investigation of intrathecal transplantation of stem cells by lumbar puncture (LP) in a rat model that simulates human thoracic spinal cord injury (SCI). Objectives: To examine the distribution and phenotype of spinal cord-derived neural stem/progenitor cells (NSPCs) and bone marrow-derived mesenchymal stromal cells (BMSCs) following LP transplantation in SCI rats. Setting: Toronto Western Research Institute, Toronto, Ontario, Canada. Methods: NSPCs or BMSCs were transplanted via LP at level L3-5 1 week after compression SCI at T8. Rats were killed at 3, 17 and 27 days after LP transplantation and the relative distribution of cells at C4, T8 and L3-5 was quantitated. The phenotype of the NSPC and BMSC was assessed with immunocytochemistry in vitro and following LP transplantation. Results: By 4 weeks, more NSPC migrated to the lesion site relative to BMSC and uninjured animals. However, there was no preferential homing of either of these types of cells into the parenchyma of the injury site, and most of the transplanted cells remained in the intrathecal space. In vitro, spinal cord-derived NSPC proliferated and expressed nestin, but after LP transplantation, NSPC became post-mitotic and primarily expressed oligodendrocyte markers. In contrast, BMSC did not express any neural antigens in vivo. Conclusion: LP is a minimally invasive method of cell transplantation that produces wide dissemination of cells in the subarachnoid space of the spinal cord. This is the first study to report and quantify the phenotype and spatial distribution of LP transplanted NSPC and BMSC in the intact and injured spinal cord.

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“…Interestingly, CD9 and CD44 staining concomitantly, which characterizes mesenchymal cells, was obtained for 1.96% to 7.27% of mononuclear cells in the present study. Considering that phenotyping is generally performed on cultured cells after the first passage (JIANG at al., 2004;JUNG et al, 2009;MaRTIN et al, 2002;SCREVEN et al, 2014), the differences in double-staining and the frequency reported in studies on CFU-F may be due to the loss of non-adherent mesenchymal cells.…”

Section: Discussionmentioning

confidence: 95%

“…CD9 has also been reported in cells of the central and peripheral nervous system (MEISTER at al., 2007). MaRTIN et al (2002) Concomitant CD9 and CD44 staining has been reported for MSC phenotyping in dogs (JUNG et al, 2009) and cats (MaRTIN et al, 2002). however, there are no studies showing the phenotypic characterization of non-cultured cells harvested from dogs.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the total bone marrow-derived mononuclear cell fraction offers a low-cost alternative to MSC culture (BRITO et al, 2010). although there are some reports on the use of non-cultured cells for therapy (SOaRES et al, 2004;BRITO et al, 2010), most studies use cultureexpanded cells (JUNG et al, 2009;QUIMBY et al, 2011). Despite kaMIShINa et al (2008) estimated the frequency of canine BMSCs to be 0.0042 (±0.0019%).…”

Section: Introductionmentioning

confidence: 99%

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