A comparison of cell survival and heat shock protein expression after radiation in normal dermal fibroblasts, microvascular endothelial cells, and different head and neck squamous carcinoma cell lines

Geyer, Fabian; Bauer, Robert; Ettl, Tobias; Schreml, Stephan; Haubner, Frank

doi:10.1007/s00784-017-2323-8

Cited by 8 publications

(7 citation statements)

References 49 publications

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“…We found that cell death in the two cell types mainly occurred after 5 Gy irradiation, with TICAE cells responding with Annexin V and Caspase 3/7 elevation while TIME cells responded with Annexin V elevation and membrane leakage as indicated by cellular dextran fluorescein positivity. The absence of Caspase 3/7 elevation in TIME cells confirms the findings in primary human microvascular endothelial cells exposed to X-rays in the 2 to 12 Gy dose range (Muschter et al, 2018). Overall, the cell death observed in our study is in line with previous findings in umbilical vein endothelial cells (Rombouts et al, 2013) and microvascular endothelial cells (Langley et al, 1997;Rousseau et al, 2011).…”

Section: Discussionsupporting

confidence: 92%

Connexin43 Hemichannel Targeting With TAT-Gap19 Alleviates Radiation-Induced Endothelial Cell Damage

et al. 2020

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Background: Emerging evidence indicates an excess risk of late occurring cardiovascular diseases, especially atherosclerosis, after thoracic cancer radiotherapy. Ionizing radiation (IR) induces cellular effects which may induce endothelial cell dysfunction, an early marker for atherosclerosis. In addition, intercellular communication through channels composed of transmembrane connexin proteins (Cxs), i.e. Gap junctions (direct cell-cell coupling) and hemichannels (paracrine release/uptake pathway) can modulate radiation-induced responses and therefore the atherosclerotic process. However, the role of endothelial hemichannel in IR-induced atherosclerosis has never been described before. Materials and Methods: Telomerase-immortalized human Coronary Artery/ Microvascular Endothelial cells (TICAE/TIME) were exposed to X-rays (0.1 and 5 Gy). Production of reactive oxygen species (ROS), DNA damage, cell death, inflammatory responses, and senescence were assessed with or without applying a Cx43 hemichannel blocker (TAT-Gap19). Results: We report here that IR induces an increase in oxidative stress, cell death, inflammatory responses (IL-8, IL-1β, VCAM-1, MCP-1, and Endothelin-1) and premature cellular senescence in TICAE and TIME cells. These effects are significantly reduced in the presence of the Cx43 hemichannel-targeting peptide TAT-Gap19. Conclusion: Our findings suggest that endothelial Cx43 hemichannels contribute to various IR-induced processes, such as ROS, cell death, inflammation, and senescence, resulting in an increase in endothelial cell damage, which could be protected by blocking these hemichannels. Thus, targeting Cx43 hemichannels may potentially exert radioprotective effects.

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Section: Discussionsupporting

confidence: 92%

Connexin43 Hemichannel Targeting With TAT-Gap19 Alleviates Radiation-Induced Endothelial Cell Damage

et al. 2020

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“…HSP27 can also mediate the effects of certain therapeutic approaches. RT increased levels of HSP27 and p-HSP27 in head and neck cancer cell lines [28], and in mouse lung in vivo [29]. Pavan and colleagues [30] found that HSP27 was required for spontaneous lung metastasis in a mouse model of ovarian cancer and inhibition of HSP27 has been shown to sensitize tumors to chemotherapy [30] and RT [31].…”

Section: Introductionmentioning

confidence: 99%

MAPKAPK2 (MK2) inhibition mediates radiation-induced inflammatory cytokine production and tumor growth in head and neck squamous cell carcinoma

et al. 2019

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Radiation therapy (RT) is a cornerstone of treatment in the management of head and neck squamous cell carcinomas (HNSCC), yet treatment failure and disease recurrence are common. The p38/MK2 pathway is activated in response to cellular stressors, including radiation, and promotes tumor inflammation in a variety of cancers. We investigated MK2 pathway activation in Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:

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“…Later, cells with similar properties were shown to reside in various tissues and organs, such as adipose tissue, umbilical cord matrix, synovial membrane, and dental tissues . A major advantage of the isolation of mesenchymal stem cells from adipose tissue is the uncomplicated acquisition of cells by liposuction or resection from subcutaneous fat tissue . Fat-derived mesenchymal stem cells are termed adipose-derived mesenchymal stem cells (ASCs) and can be expanded from liposuction material .…”

Section: Introductionmentioning

confidence: 99%

“…The volumes of lipoaspirate that are needed for procedures like vocal fold augmentation typically amount to a few milliliters. 12 This information highlights the need for new minimally invasive fat harvesting techniques. However, important factors for the clinical outcome include the amount and viability of ASCs as well as their secretory activity.…”

mentioning

confidence: 98%

“…10,11 A major advantage of the isolation of mesenchymal stem cells from adipose tissue is the uncomplicated acquisition of cells by liposuction or resection from subcutaneous fat tissue. 12 Fatderived mesenchymal stem cells are termed adiposederived mesenchymal stem cells (ASCs) and c an be expanded from liposuction material. 13,14 In 2006, the International Society for Cellular Treatment (ISCT) proposed minimal criteria for mesenchymal stem cells: the cells should adhere to plastic under standard culture conditions; be highly pure for the specific markers CD73, CD90, and CD105; and be negative for CD45, CD34, CD14 or CD11b, CD79α or CD19, and HLA-DR.…”

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confidence: 99%

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Molecular Characterization of Lipoaspirates Used in Regenerative Head and Neck Surgery

Sharaf

Kleinsasser

Schwenk‐Zieger

et al. 2019

JAMA Facial Plast Surg

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IMPORTANCE Adipose-derived mesenchymal stem cells (ASCs) have been used commonly in regenerative medicine and increasingly for head and neck surgical procedures. Lipoaspiration with centrifugation is purported to be a mild method for the extraction of ASCs used for autologous transplants to restore tissue defects or induce wound healing. The content of ASCs, their paracrine potential, and cellular potential in wound healing have not been explored for this method to our knowledge. OBJECTIVE To evaluate the characteristics of lipoaspirates used in reconstructive head and neck surgical procedures with respect to wound healing. DESIGN, SETTING, AND PARTICIPANTSThis case series study included 15 patients who received autologous fat injections in the head and neck during surgical procedures at a tertiary referral center. The study was performed from October 2017 to November 2018, and data were analyzed from October 2017 to February 2019. MAIN OUTCOMES AND MEASURESExcessive material of lipoaspirates from subcutaneous abdominal fatty tissue was examined. Cellular composition was analyzed using immunohistochemistry (IHC) and flow cytometry, and functionality was assessed through adipose, osteous, and chondral differentiation in vitro. Supernatants were tested for paracrine ASC functions in fibroblast wound-healing assays. Enzyme-linked immunosorbent assay measurement of tumor necrosis factor (TNF), vascular endothelial growth factor (VEGF), stromal-derived factor 1α (SDF-1α), and transforming growth factor β3 (TGF-β3) was performed.RESULTS Among the 15 study patients (8 [53.3%] male; mean [SD] age at the time of surgery, 63.0 [2.8] years), the stromal vascular fraction (mean [SE], 53.3% [4.2%]) represented the largest fraction within the native lipoaspirates. The cultivated cells were positive for CD73 (mean [SE], 99.90% [0.07%]), CD90 (99.40% [0.32%]), and CD105 (88.54% [2.74%]); negative for CD34 (2.70% [0.45%]) and CD45 (1.74% [0.28%]) in flow cytometry; and negative for CD14 (10.56 [2.81] per 300 IHC score) and per 300 IHC score) in IHC staining; they differentiated into osteoblasts, adipocytes, and chondrocytes. The cultivated cells showed high expression of CD44 (mean [SE], 99.78% [0.08%]) and CD273 (82.56% [5.83%]). The supernatants were negative for TNF (not detectable) and SDF-1α (not detectable) and were positive for VEGF (mean [SE], 526.74 [149.84] pg/mL for explant supernatants; 528.26 [131.79] pg/10 6 per day for cell culture supernatants) and TGF-β3 (mean [SE], 22.79 [3.49] pg/mL for explant supernatants; 7.97 [3.15] pg/10 6 per day for cell culture supernatants). Compared with control (25% or 50% mesenchymal stem cell medium), fibroblasts treated with ASC supernatant healed the scratch-induced wound faster (mean [SE]: control, 1.000 [0.160]; explant supernatant, 1.369 [0.070]; and passage 6 supernatant, 1.492 [0.094]). CONCLUSIONS AND RELEVANCEThe cells fulfilled the international accepted criteria for mesenchymal stem cells. The lipoaspirates contained ASCs that had the potential to multidifferentiate w...

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A comparison of cell survival and heat shock protein expression after radiation in normal dermal fibroblasts, microvascular endothelial cells, and different head and neck squamous carcinoma cell lines

Abstract: HNSCC use HSP to escape radiation-induced apoptosis and certain subtypes might increase proliferation after low-dose irradiation.

Cited by 8 publications

References 49 publications

Connexin43 Hemichannel Targeting With TAT-Gap19 Alleviates Radiation-Induced Endothelial Cell Damage

Connexin43 Hemichannel Targeting With TAT-Gap19 Alleviates Radiation-Induced Endothelial Cell Damage

MAPKAPK2 (MK2) inhibition mediates radiation-induced inflammatory cytokine production and tumor growth in head and neck squamous cell carcinoma

Molecular Characterization of Lipoaspirates Used in Regenerative Head and Neck Surgery

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