A comparison of chlordiazepoxide, bretazenil, L838,417 and zolpidem in a validated mouse Vogel conflict test

Mathiasen, L.; Mirza, Naheed

doi:10.1007/s00213-005-0119-z

Cited by 32 publications

(10 citation statements)

References 70 publications

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“…Our Vogel conflict method was originally validated with benzodiazepines in C57BL/6J mice, and we observed robust anxiolytic-like effects similar to those reported by Mathiasen and Mirza (2005); however, in the C57BL/6N mouse strain (parental background for the ␣ 2 ␦ mutants), benzodiazepine activity was blunted (http:// www.sfn.org/absarchive/abstract.aspx; Hain et al, 2001;Lotarski and Kinsora, 2004;Mathiasen and Mirza, 2005). It is well known that behavior and pharmacology in mice can be very sensitive to the strain used for testing (Crawley et al, 1997;Silva et al, 1997).…”

Section: Discussionsupporting

confidence: 79%

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Anxiolytic-Like Activity of Pregabalin in the Vogel Conflict Test in α₂δ-1 (R217A) and α₂δ-2 (R279A) Mouse Mutants

Lotarski

Donevan

El‐Kattan

et al. 2011

J Pharmacol Exp Ther

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The ␣ 2 ␦ auxiliary subunits (␣ 2 ␦-1 and ␣ 2 ␦-2) of voltage-sensitive calcium channels are thought to be the site of action of pregabalin (Lyrica), a drug that has been shown to be anxiolytic in clinical trials for generalized anxiety disorder. Pregabalin and the chemically related drug gabapentin have similar binding and pharmacology profiles, demonstrating high-affinity, in vitro binding to both ␣ 2 ␦-1 and ␣ 2 ␦-2 subunits. Two independent point mutant mouse strains were generated in which either the ␣ 2 ␦-1 subunit (arginine-to-alanine mutation at amino acid 217; R217A) or the ␣ 2 ␦-2 subunit (arginine-to-alanine mutation at amino acid 279; R279A) were rendered insensitive to gabapentin or pregabalin binding. These strains were used to characterize the activity of pregabalin in the Vogel conflict test, a measure of anxiolytic-like activity. Pregabalin showed robust anticonflict activity in wild-type littermates from each strain at a dose of 10 mg/kg but was inactive in the ␣ 2 ␦-1 (R217A) mutants up to a dose of 320 mg/kg. In contrast, pregabalin was active in the ␣ 2 ␦-2 (R279A) point mutants at 10 and 32 mg/kg. The positive control phenobarbital was active in mice carrying either mutation. These data suggest that the anxiolytic-like effects of pregabalin are mediated by binding of the drug to the ␣ 2 ␦-1 subunit.

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Section: Discussionsupporting

confidence: 79%

“…However, drugs that increase thirst (e.g., isoproterenol) do not necessarily increase punished responding in the mouse Vogel conflict test (Mathiasen and Mirza, 2005).…”

Section: Discussionmentioning

confidence: 95%

Anxiolytic-Like Activity of Pregabalin in the Vogel Conflict Test in α₂δ-1 (R217A) and α₂δ-2 (R279A) Mouse Mutants

Lotarski

Donevan

El‐Kattan

et al. 2011

J Pharmacol Exp Ther

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“…Significant motor effects were not observed [367]. L-838417 was also anxiolytic in murine Vogel conflict test [368]. Additionally, the compound exhibited anticonvulsant activity [369].…”

Section: L-838417mentioning

confidence: 93%

Ion Channels as Drug Targets in Central Nervous System Disorders

Waszkielewicz¹,

Gunia²,

Szkaradek³

et al. 2013

CMC

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Ion channel targeted drugs have always been related with either the central nervous system (CNS), the peripheral nervous system, or the cardiovascular system. Within the CNS, basic indications of drugs are: sleep disorders, anxiety, epilepsy, pain, etc. However, traditional channel blockers have multiple adverse events, mainly due to low specificity of mechanism of action. Lately, novel ion channel subtypes have been discovered, which gives premises to drug discovery process led towards specific channel subtypes. An example is Na + channels, whose subtypes 1.3 and 1.7-1.9 are responsible for pain, and 1.1 and 1.2 -for epilepsy. Moreover, new drug candidates have been recognized. This review is focusing on ion channels subtypes, which play a significant role in current drug discovery and development process. The knowledge on channel subtypes has developed rapidly, giving new nomenclatures of ion channels. For example, Ca 2+ channels are not any more divided to T, L, N, P/Q, and R, but they are described as Ca v 1.1-Ca v 3.3, with even newer nomenclature 1A-1I and 1S. Moreover, new channels such as P2X1-P2X7, as well as TRPA1-TRPV1 have been discovered, giving premises for new types of analgesic drugs.

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“…This test was a modification of that described by Vogel et al (1971) [26] and recently used by Mathiasen and Mirza (2005) [27]. The test cage (14.5×16×16 cm) was made from glass and had a metal grid floor.…”

Section: Vogel Conflict Test (Vct)mentioning

confidence: 99%

NMDA/glutamate mechanism of anxiolytic-like action of zinc in Vogel conflict test in mice

Abdel-Maksoud¹

2012

iosrphr

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Abstract--The objective of our study is to investigate the possible anxiolytic-like effect of zinc using one of the most useful animal models of anxiety, which is the Vogel conflict test (VCT) and to investigate the possible involvement of the glutamatergic system and NMDA receptors in this postulated effect. The anxiolytic-like effect of ZnCl 2 (20 mg/kg, i.p.) in the VCT was abolished by the pretreatment of animals with N-methyl-D-Aspartic acid (75 mg/kg, i.p., NMDA receptor agonist) that per se produced no effect in VCT. Moreover, the treatment of mice with CGP37849 (0.3 mg/kg, i.p., a competitive NMDA receptor antagonist) or MK-801 (0.05 mg/kg, i.p., a non-competitive NMDA receptor antagonist) was able to potentiate the action of sub-effective dose of ZnCl 2 (10 mg/kg, i.p.). Overall results indicate that zinc induces the anxiolytic-like effect in mice without development of tolerance and inhibition of the NMDA receptor activity is involved in the mechanism of this effect.

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A comparison of chlordiazepoxide, bretazenil, L838,417 and zolpidem in a validated mouse Vogel conflict test

Abstract: We have established the VCT in C57BL/6J mice and validated this test behaviourally, physiologically and pharmacologically. The novel GABAA receptor ligand L838,417 was anxiolytic in this mouse model, and unlike the non-selective compounds, had no effect on unpunished drinking.

Cited by 32 publications

References 70 publications

Anxiolytic-Like Activity of Pregabalin in the Vogel Conflict Test in α₂δ-1 (R217A) and α₂δ-2 (R279A) Mouse Mutants

Anxiolytic-Like Activity of Pregabalin in the Vogel Conflict Test in α₂δ-1 (R217A) and α₂δ-2 (R279A) Mouse Mutants

Ion Channels as Drug Targets in Central Nervous System Disorders

NMDA/glutamate mechanism of anxiolytic-like action of zinc in Vogel conflict test in mice

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A comparison of chlordiazepoxide, bretazenil, L838,417 and zolpidem in a validated mouse Vogel conflict test

Abstract: We have established the VCT in C57BL/6J mice and validated this test behaviourally, physiologically and pharmacologically. The novel GABAA receptor ligand L838,417 was anxiolytic in this mouse model, and unlike the non-selective compounds, had no effect on unpunished drinking.

Cited by 32 publications

References 70 publications

Anxiolytic-Like Activity of Pregabalin in the Vogel Conflict Test in α2δ-1 (R217A) and α2δ-2 (R279A) Mouse Mutants

Anxiolytic-Like Activity of Pregabalin in the Vogel Conflict Test in α2δ-1 (R217A) and α2δ-2 (R279A) Mouse Mutants

Ion Channels as Drug Targets in Central Nervous System Disorders

NMDA/glutamate mechanism of anxiolytic-like action of zinc in Vogel conflict test in mice

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Product

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Anxiolytic-Like Activity of Pregabalin in the Vogel Conflict Test in α₂δ-1 (R217A) and α₂δ-2 (R279A) Mouse Mutants

Anxiolytic-Like Activity of Pregabalin in the Vogel Conflict Test in α₂δ-1 (R217A) and α₂δ-2 (R279A) Mouse Mutants