A Comparison of Cold Storage Solutions for Pancreas Preservation Prior to Islet Isolation

Wojtusciszyn, Anne; Bosco, Domenico; Morel, Philippe; Baertschiger, Reto M.; Armanet, Mathieu; Kempf, Marie-Claude; Badet, Lionel; Toso, Christian; Berney, Thierry

doi:10.1016/j.transproceed.2005.09.019

Cited by 12 publications

(10 citation statements)

References 8 publications

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“…However, the observed results were statistically significant, so we decided to interrupt the study to avoid putting our clinical isolation program at risk without further scrutiny. During the completion of our study, the Geneva group indeed reported in a retrospective analysis successful isolation and transplantation of 7 out 19 islet preparations (37%) obtained from Celsior-perfused pancreata, a proportion slightly although not significantly inferior to that obtained by the same authors with UW-perfused organs (35). In our study, we found that the qualitative characteristics of islet preparations including viability and insulin secretion were not significantly different between the two groups.…”

Section: Discussionsupporting

confidence: 53%

“…In our study, we found that the qualitative characteristics of islet preparations including viability and insulin secretion were not significantly different between the two groups. There is therefore little doubt that successful islet isolation and transplantation is not restricted to organs perfused and stored with UW, as shown by the Geneva group (35).…”

Section: Discussionmentioning

confidence: 99%

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Influence of Preservation Solution on Human Islet Isolation Outcome

Hübert

Gmyr²,

Arnalsteen³

et al. 2007

Transplantation

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Our results suggest that colloid-free preservation solutions might be suboptimal for pancreas perfusion and cold storage prior to islet isolation and transplantation. Because pancreata are now frequently recovered for islet transplantation, preliminary experimental and clinical data about islet isolation should be obtained prior to the routine implementation of new preservation solutions for abdominal perfusion during multiorgan recovery.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Influence of Preservation Solution on Human Islet Isolation Outcome

Hübert

Gmyr²,

Arnalsteen³

et al. 2007

Transplantation

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“…Several major studies and clinical trials have demonstrated the clinical equivalence of HTK when compared to UW for abdominal organ preservation and transplant outcomes (12,20,24,29) and specifically for whole pancreas transplantation (4,10,11). Notably, since 1995, a number of studies have assessed the ability of HTK to preserve pancreata intended for islet isolation, with results similar to those obtained with other organ preservation solutions and paralleling those demonstrated for other abdominal organs (7,15,16,30). However, these studies have been limited either by small sample size or incomplete analysis of isolation outcomes.…”

Section: Introductionmentioning

confidence: 67%

“…In the preservation of pancreata intended for islet isolation, a number of studies comparing the efficacy of HTK with that of other preservation solutions have been conducted since 1995, demonstrating results similar to those obtained in other abdominal organs (11-14). However, these studies have been limited either by small sample size or incomplete analysis of isolation outcomes.…”

Section: Introductionmentioning

confidence: 83%

Histidine-Tryptophan-Ketoglutarate and University of Wisconsin Solution Demonstrate Equal Effectiveness in the Preservation of Human Pancreata Intended for Islet Isolation: A Large-Scale, Single-Center Experience

Paushter

Danielson

et al. 2013

Cell Transplant

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We have previously reported that both Histidine-Tryptophan-Ketoglutarate solution (HTK) and University of Wisconsin solution (UW) provide equal preservation of the pancreas for islet isolation, based upon the assessment of islet yield and function. In this study, we further assessed the ability of each solution to prevent cellular edema. This large-scale, retrospective analysis included the assessment of islet isolation outcomes influenced by cellular edema, such as pancreatic digestion efficacy, purification outcome, and islet size distribution. Multi-variable linear regression analysis, adjusted for donor age, sex, BMI, cold ischemia time, and enzyme, demonstrated similar results for the HTK group (n = 95) and the UW group (n = 157), including post-purification islet yields (the HTK: 289,702 vs. the UW: 283,036; p = 0.76), percentage of digested pancreatic tissue (the HTK: 66.9% vs. the UW: 64.1%; p = 0.18), and islet loss from post-digestion to post-purification (the HTK: 24,972 vs. the UW: 39,551; p = 0.38). Changes in islet size between the post-digestion and post-purification stages were comparable within each islet size category for the HTK and the UW groups (p = 0.14 - 0.99). Tissue volume distribution across purification fractions and islet purity in the top fractions were similar between the groups; however, the HTK group had significantly higher islet purity in the middle fractions (p = 0.003 - 0.008). Islet viability and stimulation indices were also similar between the HTK and the UW groups. In addition, we analyzed a small sample of patients transplanted either with HTK (n = 7) or UW (n = 8) preserved islets and found similar outcomes. This study demonstrates that HTK and UW solutions offer comparable pancreas preservation and are equally efficacious in the prevention of pancreatic tissue edema in islet transplantation. Future studies assessing in vivo islet outcomes in larger samples are needed for complete analysis of the effects of HTK on islet transplantation.

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“…Other preservation solutions used include Celsior, HTK (histidine-tryptophan-ketoglutarate), Institut-George-Lopes (IGL)-1 and others (Hubert et al 2007;Kaddis et al 2010;Manrique et al 2006;Noguchi et al 2008). The flushing step cools the pancreas and removes debris and thrombi (Baertschiger et al 2008;Hubert et al 2007;Kinasiewicz and Fiedor 2003;Salehi et al 2006;White et al 2001;Wojtusciszyn et al 2005).…”

Section: Pancreas Perfusion and Transport Factorsmentioning

confidence: 99%

Islet Transplantation: Factors in Short-Term Islet Survival

Deters

Stokes

Gunton

2011

Arch. Immunol. Ther. Exp.

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Islet transplantation has the potential to cure type 1 diabetes. In recent years, the proportion of patients achieving initial insulin independence has improved, but longer term outcomes remain poor compared to those for whole pancreas transplants. This review article will discuss factors affecting islet yield and viability leading up to transplantation and in the immediate post-transplant period.

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A Comparison of Cold Storage Solutions for Pancreas Preservation Prior to Islet Isolation

Cited by 12 publications

References 8 publications

Influence of Preservation Solution on Human Islet Isolation Outcome

Influence of Preservation Solution on Human Islet Isolation Outcome

Histidine-Tryptophan-Ketoglutarate and University of Wisconsin Solution Demonstrate Equal Effectiveness in the Preservation of Human Pancreata Intended for Islet Isolation: A Large-Scale, Single-Center Experience

Islet Transplantation: Factors in Short-Term Islet Survival

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