This article presents a comparative analysis of the properties of cotton yarn spun on aerodynamic compact spinning and open-end rotor spinning systems. Yarn samples with a linear density of 50 Tex, 37 Tex, 30 Tex, 25 Tex, and 20 Tex were spun both on the aerodynamic compact and rotor spinning systems using the same finisher drawn sliver of medium staple cotton which were produced by a specific mixing. The quality parameters such as mass variation, imperfection index, hairiness, and tensile behavior (strength in count strength product, elongation percentage) of the yarn samples were assessed and analyzed. The results revealed that aerodynamic compact spun yarn had a lower unevenness and mass variation, a higher imperfection in case of a finer count, less hairiness, higher tensile strength, and lower elonga-tion% compared to the open-end rotor spun yarn samples. Finally, pairedsamples t-test and regression analysis were carried out by using IBM SPSS 25 to check the significance of yarn quality parameters and correlation among them.