A comparison of demyelinating and myelination-inhibiting factor induction by whole peripheral nerve tissue and P2 protein

Seil, Fredrick J.; Kies, Marian W.; Bacon, Martha

doi:10.1016/0006-8993(81)90924-0

Cited by 18 publications

(8 citation statements)

References 26 publications

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“…The liposomes were washed by centrifugation in a Sorvall microfuge after the addition of 1 ml ofGVB2+ and resuspended in 1 ml GVB2". 20 Ml of each liposome preparation was used in the Cl fixation and transfer assay for detection of Ab binding. The control liposomes contained egg lecithin and cholesterol.…”

Section: Methodsmentioning

confidence: 99%

“…P2 and galactocerebroside both induce experimental neuritis in rats and rabbits (18,19). Ab to P2 a basic protein which is not exposed on the myelin surface does not demyelinate explants in vitro (20) while Ab to the surface determinant galactocerebroside causes in vivo and vitro demyelination (21,22) and mediates cytoxicity of Schwann cells (23). GBS serum also mediates demyelination in vivo and in vitro (24)(25)(26).…”

Section: Introductionmentioning

confidence: 99%

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Anti-peripheral nerve myelin antibodies in Guillain-Barre syndrome bind a neutral glycolipid of peripheral myelin and cross-react with Forssman antigen.

Koski

Chou²,

Jungalwala³

1989

J. Clin. Invest.

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During acute-phase illness, serum of patients with GuillainBarre syndrome (GBS) contain complement-fixing antibodies (Ab) to peripheral nerve myelin (PNM). We investigated PNM lipids as putative antigens for these Ab since GBS serum retained significant reactivity to PNM treated with protease. Ab binding to specific lipids was studied with a C1 fixation and transfer (CFIT) assay using fractions of PNM lipid reincorporated into liposomes as antigen targets or to lipids on HPTLC plates with peroxidase-labeled goat Ab to human IgM. Reactivity was detected to a neutral glycolipid (NGL) of human PNM with a similar number of carbohydrates residues to that of Forssman hapten (Forss). Anti-NGL Ab titers in GBS patients (50-220 U/ml) were significantly elevated over disease and normal controls (0-5 and 0-6 U/ml). We studied possible antigenic cross-reactivity of these Ab with Forss by first quantitating Ab activity with CiFF assay and liposomes containing Forss. All 12 GBS sera tested showed titers (54-272 U/ml) significantly elevated over 11 disease controls (0-22 U/ml) and 25 normal controls (0-11 U/mi). GBS serum Ab reacted with Forss isolated from dog nerve or sheep erythrocytes on HPTLC plates. Further, absorption of 80-100% of anti-NGL Ab activity and 17-97% of anti-PNM Ab activity from eight GBS patient serums was accomplished with liposomes containing Forss but not with control liposomes. In seven GBS patients anti-NGL Ab activity represented only a portion of anti-PNM Ab activity. These results suggest that a glycolipid with antigenic cross-reactivity to Forssman hapten may be responsible for some of the anti-PNM Ab activity in GBS.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anti-peripheral nerve myelin antibodies in Guillain-Barre syndrome bind a neutral glycolipid of peripheral myelin and cross-react with Forssman antigen.

Koski

Chou²,

Jungalwala³

1989

J. Clin. Invest.

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“…A PNS equivalent of EAE is experimental allergic neuritis (EAN), which can be induced with the inoculation of FCA plus whole PNS tissue [52] or with the P 2 fraction of PNS myelin [53,54]. Yonezawa et al [55] reported the demyelination of PNS cultures by rabbit and guinea pig antisera directed against whole PNS tissue, a finding confirmed in later studies [56,57,58]. We additionally reported the inhibition of peripheral myelination by anti-PNS sera [58].…”

Section: Pns Myelin Antigens and Eanmentioning

confidence: 99%

Myelin Antigens and Antimyelin Antibodies

Seil

2018

Antibodies

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Abstract:The purpose of this review is to provide an historical perspective on studies of serum derived antimyelin antibodies. Antimyelin antibodies can be defined by their action on myelinating organotypic nervous system tissue cultures and include demyelinating antibodies, which have destructive effects on myelin when applied to already myelinated cultures, and myelination inhibiting antibodies, which prevent myelin formation when applied to cultures prior to myelination. Myelin antigens were evaluated in animal studies for their ability to induce experimental allergic encephalomyelitis, an inflammatory demyelinating disease, and correlated with the induction of antimyelin antibodies. As tissue culture demyelinating activity was also found in sera from some patients with multiple sclerosis, a human inflammatory demyelinating disease, studies were undertaken to characterize the nature of the demyelinating factors.

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“…Myelin-induced EAN serum and antigalactocerebroside serum demyelinate PNS as weil as CNS cultures or inhibit myelination in these cultures if included in the growth medium (T. Saida et al, 1979c;Seil et al, 1981). Pretreatment of the serum with galactocerebroside blocks this activity Raineet al, 1981).…”

Section: Antibody Response To Pns Myelin Lipidsmentioning

confidence: 99%

“…Such is not the case with P 2 -induced EAN. Serum from animals with P2-induced EAN does not contain any demyelinating or myelination-inhibition activity against CNS or PNS organ-specific tissiIe cultures (Seil et al, 1981) and does not contain antigalactocerebroside antibody (Hughes et al, 1981). Antibodies to PI or P2 do not demyelinate PNS cultures either (Mithen et al, 1982a,b).…”

Section: Antibody Response To Pns Myelin Lipidsmentioning

confidence: 99%

Myelin

1984

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A comparison of demyelinating and myelination-inhibiting factor induction by whole peripheral nerve tissue and P2 protein

Cited by 18 publications

References 26 publications

Anti-peripheral nerve myelin antibodies in Guillain-Barre syndrome bind a neutral glycolipid of peripheral myelin and cross-react with Forssman antigen.

Anti-peripheral nerve myelin antibodies in Guillain-Barre syndrome bind a neutral glycolipid of peripheral myelin and cross-react with Forssman antigen.

Myelin Antigens and Antimyelin Antibodies

Myelin

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