A comparison of diagnosis of pregnancy in the goat via transrectal ultrasound scanning, progesterone, and pregnancy-associated glycoprotein assays

González, F.; Cabrera, Fernando; Batista, M.; Rodríguez, Nathalie; Alamo, D; Sulon, J.; Beckers, J.F.; Gracia, A.

doi:10.1016/j.theriogenology.2003.12.009

Cited by 62 publications

(47 citation statements)

References 12 publications

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“…According to Gonzalez et al (2004) detectable blood plasma PAGs concentrations in goats appear around the 25-26 day of gestation. Shahin et al (2013) reported statistically significant differences in PAGs levels by the 21 st day post breeding between pregnant and nonpregnant goats.…”

Section: Pregnancy Associated Glycoproteinsmentioning

confidence: 99%

“…The pregnancy diagnosis in goats is essential for better efficacy and management of reproduction (Doize et al, 1997), providing information about conception rates after artificial insemination (Matsas, 2007), gestation course (Amer, 2010), time for drying-off and parturition date (Doize et al, 1997;Gonzalez et al, 2004). The separation of pregnant and nonpregnant does in different groups reduces the losses from abortions, stillbirths or giving birth to non-viable offspring and optimises labour, feed and medication costs (Wani et al, 1998).…”

mentioning

confidence: 99%

“…A method, easy to perform, reliable, not expensive and with high accuracy is preferable. The selection of a method depends on the stage of gestation, method's precision and the available equipment at the farm (Gonzalez et al, 2004). From the point of view of their practical application potential, Karen et al (2001) divide the methods for pregnancy diagnosis in goats to less practical (non-return to oestrus, transabdominal palpation, laparoscopy, laparotomy, palpation of the caudal uterine artery, rosette inhibition test) and widely used (radiography, rectoabdominal palpation, hormonal assays, assay of pregnancy proteins, ultrasonography).…”

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confidence: 99%

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Pregnancy diagnosis techniques in goats – a review

Karadaev¹

2015

BJVM

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SummaryKaradaev, M., 2015. Pregnancy diagnosis techniques in goats -a review. Bulg. J. Vet. Med., 18, No 3,[183][184][185][186][187][188][189][190][191][192][193] Several techniques for pregnancy diagnosis in goats are developed. Some of them are efficient and applicable in the field, but not enough accurate. Others are outlined with a high precision, but require equipment and specialised skills. Laparoscopy, laparotomy and vaginal biopsy are accurate but invasive and thus, inappropriate for the routine practice. Abdominal inspection, transabdominal palpation and increased live weight could be indicative for pregnancy in goats but they are reliable only after the second half of gestation. Udder examination and palpation are of low accuracy and their independent application for pregnancy diagnosis is not suitable. Blood, milk and faecal progesterone assays could indicate pregnancy in goats after the 21 st post insemination day until the end of gestation. The detection of pregnancy associated glycoproteins (PAGs) in blood plasma or milk samples is an early pregnancy marker but requires laboratory equipment. Non-return to oestrus is a cheap, practical and widely used method for detection of pregnancy between post insemination days 17 and 21 and does without signs of oestrus are assumed to be pregnant. A-mode, B-mode and Doppler ultrasonography are alternative methods for pregnancy diagnosis. Their accuracy and practical application potential are different. The two-dimensional ultrasound is one of the most appropriate methods for pregnancy diagnosis in goats. The accuracy of the method is about 100% on post insemination day 25-30 using the transrectal approach, whereas via the transabdominal approach, this accuracy is attained between the 40 th and the 45 th day.

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Section: Pregnancy Associated Glycoproteinsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Pregnancy diagnosis techniques in goats – a review

Karadaev¹

2015

BJVM

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“…wood bison [107], moose [108], rocky mountain elk [109], white-tailed deer [110,111], mule deer [111], red deer [112], sika deer [113], fallow deer [114,115], mountain goat [116] and Alpine goats [117][118][119]. The measurement of PAG protein concentrations in maternal blood or milk of ruminants allow for 76.6-100% accuracy rates for early pregnancy diagnoses [120,121] and provide an alternative method to ultrasonography for determining late embryonic mortality [96,122].…”

Section: Pregnancy Diagnostic Pag Testsmentioning

confidence: 99%

Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) – a review

2006

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-This review presents a broad overview of chorionic glycoproteins encoded by the Pregnancy-Associated Glycoprotein (PAG) gene family and also serves to illustrate how the recent discovery of the PAG family has contributed to our general knowledge of genome evolution, placental transcription and placental protein expression. The complex and large PAG family is restricted to the Artiodactyla order, although single PAG-like genes have also been identified in species outside the Artiodactyla. The PAGs are members of the aspartic proteinase (AP) superfamily. Unexpectedly, however, some members of the PAG family possess amino acid substitutions within and around the active site that likely render them unable to act as proteinases. This paper summarises the available information regarding biodiversity of PAG gene expression based on cDNA cloning, mRNA localisation studies and the structural organisation of the PAG genes with a particular emphasis on PAG promoters. It also compares available data regarding PAG protein purifications, sequencing and their N-glycodiversity. Finally, it discusses the scientific relevance, possible functional roles of the PAGs and describes possible profitable applications related to the detection of PAG proteins in the blood of pregnant domestic and wild species. chorionic glycoproteins / eutherian PAG family / diagnostic PAG markers

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“…Ultrasonography has been used with great deal of accuracy as a mean for pregnancy diagnosis and estimation of fetal numbers in ewes and does (Martinez et al, 1998;Gonzales et al, 2004). In the present study, the first sign of pregnancy in ewes and does was the presence a circular or elongated echogenic structure located in uterus cranial to the urinary bladder on day 21 post service.…”

Section: Discussionmentioning

confidence: 61%

CIDR and Ultrasound-Fetometry Scan; Comparative Study Between Sheep and Goat in Egypt

Ahmed¹,

El-Naeim²,

Ibrahim³

2017

AJVS

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Key words:CIDR, CRL, BPD, FOD, Progesterone, PMSG, Ultrasound.The present study aims to evaluate the impact of controlled internal drug release (CIDR) in combination with prostaglandin F2α (PGF2α) and pregnant mare serum gonadotropin (PMSG) treatments on reproductive performance of dairy ewes and does besides, evaluation the accuracy of pregnancy diagnosis during the breeding season. Twenty female animals divided as Ossimi ewes (n=10) and Baladi does (n=10) were subjected to ovsynch regimen. Ultrasonographic examinations were conducted weekly using realtime B-mode ultrasound scanner by transrectal linear and transabdominal convex probes starting from day 14 post service. This study showed that estrus response was 100% in both ewes and does. The conception rates were 90% in ewes and 80 % in does. The incidence of twining was higher in ewes when compared to that in does with incidence rate 67% and 50 in ewes and does, respectively. The accuracy of ultrasound examination for determining fetal number was 100% for single and twin pregnancy. The birth rate was 100 % for both species; and this was confirmed at lambing / kidding. In conclusion, our results indicate that using biotechnologies such as ovsynch regimen and pregnancy diagnosis by ultrasonography are useful to improve the management of reproduction in ewes and does.

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A comparison of diagnosis of pregnancy in the goat via transrectal ultrasound scanning, progesterone, and pregnancy-associated glycoprotein assays

Cited by 62 publications

References 12 publications

Pregnancy diagnosis techniques in goats – a review

Pregnancy diagnosis techniques in goats – a review

Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) – a review

CIDR and Ultrasound-Fetometry Scan; Comparative Study Between Sheep and Goat in Egypt

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