A comparison of distinct bone marrow-derived cells on cartilage tissue engineering

Chen, Chia Chun; Hsiao, Chu Yun; Wang, Yao Horng; Chen, Yi Chun; Chang, Chih Hung; Fang, Hsu Wei

doi:10.1016/j.jtice.2017.05.022

Cited by 3 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They mixed porcine cartilage, SDSCs fragments with BMSCs or BMC to form different constructs. Results showed that BMC-containing constructs could stimulate chondrogenesis and BMSCs-containing constructs could assist in ECM synthesis [75] .…”

Section: Mscs For Cartilage Defect Treatmentmentioning

confidence: 99%

Clinical application of mesenchymal stem cells for cartilage regeneration

Chen¹,

Chang²

2020

Plast Aesthet Res

Self Cite

View full text Add to dashboard Cite

Cartilage has the ability to transmit and distribute loads, providing lubrication in the diarthrodial joints. Risk factors including age, gender, genetics, nutrition and bone density may predispose to osteoarthritis (OA) and cartilage defect formation. Appropriate treatment include sufficient rest and medical therapy. Intra-articular injections such as steroids, platelet-rich plasma, visco-supplementation and mesenchymal stem cells (MSCs) injections present as alternative options for non-operative treatments. For cartilage defects, microfracture (MF), osteochondral autograft transplantation (OAT) and autologous chondrocyte implantation (ACI) are the most common treatment procedures. MSCs have been identified as an ideal cell source for OA therapy because they are easily expanded in culture, generally non-tumorigenic, and can be readily obtained from patients. It may be harvested from bone marrow (BMSCs), adipose tissue (ADSCs), synovium (SDSCs) or peripheral blood. BMSCs features the most common source of stem cells, and infrapatellar fat pad (IPFP) is another popular stem cell source. A phase 1 clinical study entitled "Treatment of Knee OA with Autologous Mesenchymal Stromal Cell Product (RegStem®)" was conducted in Taiwan and utilized 5 × 107 IPFP-MSCs in the study for OA therapy. Most of the existing clinical studies have shown that patients receiving MSCs treatment have improved clinical outcome, such as Visual Analogue Scale, International Knee Documentation Committee and Western Ontario and McMaster Universities Arthritis Index (WOMAC) score. Some studies have also found an improvement in cartilage volume by Magnetic Resonance Imaging evaluation. Furthermore, MSCs can also be used for cartilage defect treatment. Clinical outcomes such as IKDC, Lysholm, and Tegner scores showed significant improvement when the cartilage defects were repaired and regenerated by several millions of stem cells. A 10-year follow-up clinical research indicated that there was no apparent increased tumor formation risk when BMSCs were used for cartilage defect treatment.

show abstract

Section: Mscs For Cartilage Defect Treatmentmentioning

confidence: 99%

Clinical application of mesenchymal stem cells for cartilage regeneration

Chen¹,

Chang²

2020

Plast Aesthet Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition to the biomaterial determination for scaffold preparation, accurately selecting of various available cells are vital [15,16]. Chen et al [17] studied the effect of utilizing diverse bone marrow-derived cell sources, including bone marrow concentrate (BMC) and BMSC for cartilage tissue engineering. The biological constructs containing BMC/BMSCs and articular tissue fragments were examined in vitro.…”

Section: Introductionmentioning

confidence: 99%

An in-silico study of cartilage tissue engineering and its primary developmental growth factors in a systems biology approach

Beheshtizadeh¹

2020

Preprint

View full text Add to dashboard Cite

Abstract Background Numerous growth factors work in consonance to regulate the development and homeostasis of engineered cartilage. In order to fabricate mature cartilages provided to implantation, developing biological processes should be known and employed. In this regard, a systems biology study could be considered as an inexpensive evaluation method. In this work, a systems biology study was performed on 11 protein-coding genes participating in cartilage development. Results Developing a protein-protein interaction (PPI) network resulted in a graph with 11 nodes and 28 edges. Additionally, a gene ontology (GO) analysis and a centrality analysis were performed based on the degree index. Four most crucial biological processes involved in cartilage engineering identified by the gene ontology analysis: cartilage development, cartilage condensation, regulation of cartilage development, and cartilage development involved in endochondral bone morphogenesis. Also, seven most interactive proteins were detected performing a degree-based centrality analysis: COL2A1, SOX9, CTGF, BMP7, TGFB1, CD44, and MAPK14. Conclusion Cartilage developmental proteins are efficient in forming and developing extracellular matrix and cartilage development, from its formation to the mature structure. However, this in-silico study recommends four most important developmental growth factors applicable to cartilage tissue engineering: COL2A1, SOX9, CTGF, and TGFB1.

show abstract

Identification of Regeneration-Involved Growth Factors in Cartilage Engineering Procedure Promotes its Reconstruction

Azami

Beheshtizadeh

2021

Regen. Med.

View full text Add to dashboard Cite

Aim: To fabricate mature cartilage for implantation, developmental biological processes and proteins should be understood and employed. Methods: A systems biology study of all protein-coding genes participating in cartilage regeneration resulted in a network graph with 11 nodes and 28 edges. Gene ontology and centrality analysis were performed based on the degree index. Results: The four most crucial biological processes along with the seven most interactive proteins involved in cartilage regeneration were identified. Some proteins, which are under serious discussion in cartilage developmental and disease processes, are included in regeneration. Conclusions: Findings positively correlate with the literature, supporting the use of the four most impressive proteins as growth factors applicable to cartilage tissue engineering, including COL2A1, SOX9, CTGF, and TGFβ1.

show abstract

A comparison of distinct bone marrow-derived cells on cartilage tissue engineering

Cited by 3 publications

References 25 publications

Clinical application of mesenchymal stem cells for cartilage regeneration

Clinical application of mesenchymal stem cells for cartilage regeneration

An in-silico study of cartilage tissue engineering and its primary developmental growth factors in a systems biology approach

Identification of Regeneration-Involved Growth Factors in Cartilage Engineering Procedure Promotes its Reconstruction

Contact Info

Product

Resources

About