A comparison of DNA‐based methods for delimiting species in a Cretan land snail radiation reveals shortcomings of exclusively molecular taxonomy

Sauer, Jan; Hausdorf, Bernhard

doi:10.1111/j.1096-0031.2011.00382.x

Cited by 85 publications

(85 citation statements)

References 108 publications

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“…Indeed, a shortcoming of this method is that a single species with a strong spatial population structure could be wrongfully split into several separate GMYC lineages (Pons et al 2006). Sauer and Hausdorf (2012) recommended using multilocus markers but they admit that even this approach has its limits in disentangling species within a single cluster. The application of the PTP model (based on the PSC) on the nuclear markers and the combined dataset (cytb and nuclear markers) resulted in either one or five hazel dormouse species, respectively.…”

Section: Species Delimitationmentioning

confidence: 99%

Evolutionary history and species delimitations: a case study of the hazel dormouse, Muscardinus avellanarius

Mouton

Mortelliti

Grill³

et al. 2016

Conserv Genet

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Robust identification of species and significant evolutionary units (ESUs) is essential to implement appropriate conservation strategies for endangered species. However, definitions of species or ESUs are numerous and sometimes controversial, which might lead to biased conclusions, with serious consequences for the management of endangered species. The hazel dormouse, an arboreal rodent of conservation concern throughout Europe is an ideal model species to investigate the relevance of species identification for conservation purposes. This species is a member of the Gliridae family, which is protected in Europe and seriously threatened in the northern part of its range. We assessed the extent of genetic subdivision in the hazel dormouse by sequencing one mitochondrial gene (cytb) and two nuclear genes (BFIBR, APOB) and genotyping 10 autosomal microsatellites. These data were analysed using a combination of phylogenetic analyses and species delimitation methods. Multilocus analyses revealed the presence of two genetically distinct lineages (approximately 11 % cytb genetic divergence, no nuclear alleles shared) for the hazel dormouse in Europe, which presumably diverged during the Late Miocene. The phylogenetic patterns suggests that Muscardinus avellanarius populations could be split into two cryptic species respectively distributed in western and central-eastern Europe and Accession numbers are available at the ''European Nucleotide Archive'' browser at the address http://www.ebi.ac.uk/ena/data/view/ LT614830-LT614893 and in Supplementary Anatolia. However, the comparison of several species definitions and methods estimated the number of species between 1 and 10. Our results revealed the difficulty in choosing and applying an appropriate criterion and markers to identify species and highlight the fact that consensus guidelines are essential for species delimitation in the future. In addition, this study contributes to a better knowledge about the evolutionary history of the species.

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Section: Species Delimitationmentioning

confidence: 99%

Evolutionary history and species delimitations: a case study of the hazel dormouse, Muscardinus avellanarius

Mouton

Mortelliti

Grill³

et al. 2016

Conserv Genet

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“…Methods for species delimitation using genetic data recommend identifying clusters of organisms with similar genotypes (Bradley & Baker, 2006;Hausdorf, 2011). However, irrefutable criteria to recognize clusters representing species from others are still lacking (Sauer & Hausdorf, 2012).…”

Section: Species Delimitation and Specific Status Of Gmyc Lineagesmentioning

confidence: 99%

Diversity and endemism of Murinae rodents in Thai limestone karsts

Latinne¹,

Waengsothorn²,

Rojanadilok³

et al. 2013

Systematics and Biodiversity

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This study aims to investigate the species diversity of rodents living in karst ecosystems of Thailand. A survey has been conducted throughout Thailand, 122 karsts sampled and 477 Murinae rodents live-trapped. Phylogenetic reconstructions were carried out using two mitochondrial markers (cytb, COI). A sequence-based species delimitation method completed by the analysis of the level of genetic divergence was then applied to define species boundaries within our dataset. The phylogenetic position of Niviventer hinpoon was also investigated and sequences obtained from the holotype specimen of this species were used to reliably identify samples of N. hinpoon. A total of 12 described Murinae species, corresponding to 17 deeply divergent genetic lineages, were encountered in limestone karsts of Thailand. Our study revealed an important genetic diversity within the traditionally recognized species Maxomys surifer (four highly divergent genetic lineages), Leopoldamys neilli (two highly divergent genetic lineages) and Berylmys bowersi (two highly divergent genetic lineages). These species could be considered as species complex and require further taxonomic work. This study also provides valuable information on the distribution of the two rodent species endemic to limestone karsts of Thailand, L. neilli and N. hinpoon. Leopoldamys neilli was the most abundant species encountered in Thai karsts during our survey. However, L. neilli specimens from western Thailand are genetically highly divergent from the remaining populations of L. neilli and could represent a separate species. Niviventer hinpoon, phylogenetically closely related to N. fulvescens, is much rarer and its distribution limited to central Thailand. Most of the other captured species are typically associated with forest ecosystems. This study suggests that limestone karsts play a key role in the preservation of the rodent species endemic to such habitat, but they would also provide refuges for the forest-dwelling Murinae rodents in deforested regions.

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“…The introduction of molecular genetic methods in biological systematics has often contributed to solving taxonomic problems. This approach has, however, frequently caused even more confusion by revealing more complex patterns of hitherto unnoticed genetic variation and differentiation of mitochondrial (mt) clades (Sauer & Hausdorf, 2012). …”

Section: Introductionmentioning

confidence: 99%

Differentiation in the Trochulus hispidus complex and related taxa (Pulmonata: Hygromiidae): morphology, ecology and their relation to phylogeography

Duda

Kruckenhauser

Sattmann

et al. 2014

Journal of Molluscan Studies

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In this study we investigated the morphology and ecology of representatives of the taxonomically ambiguous genus Trochulus. The main focus was on the T. hispidus complex, which comprises several genetically highly divergent mitochondrial clades, as determined in a parallel molecular genetic study. We analysed shell morphology and anatomical traits and asked whether the clades are differentiated in these characters. In addition, the related species T. oreinos and T. striolatus were investigated and compared with the T. hispidus complex. Finally, we compared the ecological requirements of the taxa. Among the genetic clades of the T. hispidus complex there was no clear morphological differentiation and geographic populations could not be distinguished based on their morphology. The investigated characters of the genital anatomy did not allow discrimination of any of the T. hispidus clades and were not even diagnostic for the group as a whole. The morphotype of T. sericeus is present in all clades and thus cannot be assigned to a genetic group or any specific population. Thus, our morphological data do not provide evidence that any of the mitochondrial T. hispidus clades represent separate species. Concerning interspecific delimitation, the T. hispidus complex was clearly differentiated from T. striolatus and T. oreinos by shell morphological and anatomical characters, e.g. sculpture of shell surface and details of the penis. Finally, the habitat of T. oreinos is different from those of the other two species. In contrast to the lack of correspondence between genetic and morphological differentiation within the T. hispidus complex, related species display intraspecific morphological differentiation corresponding with mitochondrial clades: within T. striolatus there was a slight morphological differentiation between the subspecies T. s. striolatus, T. s. juvavensis and T. s. danubialis. The two subspecies of T. oreinos could be discriminated by a small but consistent difference in the cross-section of the penis. The unequal levels of intraspecific differentiation are caused by different evolutionary histories as a consequence of disparities in ecological demands, dispersal ability and use of glacial refugia: both the T. hispidus complex and T. striolatus are fast-spreading, euryoecious organisms which are able to (re-)colonize habitats and survive under different climate conditions. While the T. hispidus complex probably survived the Pleistocene in several glacial refugia, for T. striolatus one glacial refugium is suggested. Trochulus oreinos differs from the other taxa, as it is a slow disperser with a narrow ecological niche. We suggest that its subspecies spent at least the last glaciation in or close to the presently inhabited areas.

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A comparison of DNA‐based methods for delimiting species in a Cretan land snail radiation reveals shortcomings of exclusively molecular taxonomy

Cited by 85 publications

References 108 publications

Evolutionary history and species delimitations: a case study of the hazel dormouse, Muscardinus avellanarius

Evolutionary history and species delimitations: a case study of the hazel dormouse, Muscardinus avellanarius

Diversity and endemism of Murinae rodents in Thai limestone karsts

Differentiation in the Trochulus hispidus complex and related taxa (Pulmonata: Hygromiidae): morphology, ecology and their relation to phylogeography

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