A comparison of extended spectrum β-lactamase producing Escherichia coli from clinical, recreational water and wastewater samples associated in time and location

Jørgensen, Silje Bakken; Søraas, Arne; Arnesen, Lotte P. Stenfors; Leegaard, Truls Michael; Sundsfjord, Arnfinn; Jenum, Pål A.

doi:10.1371/journal.pone.0186576

Cited by 75 publications

(78 citation statements)

References 48 publications

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“…This sub-clone has spread as a pandemic among humans, often identified as the pathogen in severe bloodstream infections [ 33 ]. Secondly, ST617, ST38 and ST10, found in one, two and seven gulls respectively, are also sequence types with clinical importance in human medicine [ 34 – 37 ]. A recent study also described high relatedness of clinical isolates of ST38 from UK and one isolate of ST38 isolated from a Mongolian wild bird [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…In this study, the isolates from gulls and humans showed high similarities to those originating from Swedish surface waters, suggesting WWTPs as one possible source. Comparable a recent Norwegian study described that ST131, ST10 and ST38 carrying the genes bla CTX-M-1 , bla CTX-M-14 and bla CTX-M-15 were frequently identified from surface waters, wastewaters and human UTIs [ 37 ]. The same gene and MLST combinations were also frequent in Swedish gulls, humans and surface waters ( Table 1 , Fig 1 , Fig 3 ).…”

Section: Discussionmentioning

confidence: 99%

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ESBL-producing Escherichia coli in Swedish gulls—A case of environmental pollution from humans?

Atterby

Börjesson

et al. 2017

PLoS ONE

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ESBL-producing bacteria are present in wildlife and the environment might serve as a resistance reservoir. Wild gulls have been described as frequent carriers of ESBL-producing E. coli strains with genotypic characteristics similar to strains found in humans. Therefore, potential dissemination of antibiotic resistance genes and bacteria between the human population and wildlife need to be further investigated. Occurrence and characterization of ESBL-producing E. coli in Swedish wild gulls were assessed and compared to isolates from humans, livestock and surface water collected in the same country and similar time-period. Occurrence of ESBL-producing E. coli in Swedish gulls is about three times higher in gulls compared to Swedish community carriers (17% versus 5%) and the genetic characteristics of the ESBL-producing E. coli population in Swedish wild gulls and Swedish human are similar. ESBL-plasmids IncF- and IncI1-type carrying ESBL-genes blaCTX-M-15 or blaCTX-M-14 were most common in isolates from both gulls and humans, but there was limited evidence of clonal transmission. Isolates from Swedish surface water harbored similar genetic characteristics, which highlights surface waters as potential dissemination routes between wildlife and the human population. Even in a low-prevalence country such as Sweden, the occurrence of ESBL producing E. coli in wild gulls and the human population appears to be connected and the occurrence of ESBL-producing E. coli in Swedish gulls is likely a case of environmental pollution.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

ESBL-producing Escherichia coli in Swedish gulls—A case of environmental pollution from humans?

Atterby

Börjesson

et al. 2017

PLoS ONE

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“…The whole genome sequencing of C. difficile strains, which are a commensal organism and a pathogen in both human and animal [39], showed that C. difficile isolates with identical (no SNP differences) and almost identical (less than two SNP differences) are detected in both farmers and pigs in the Netherlands [34]. Many β-lactam-resistant E. coli strains identified in human samples were also detected in animals [29,31,[35][36][37][38] and environment [35,40,41]. These results indicate the complex transmission of β-lactam-resistant E. coli strains among human, animal, and environment.…”

Section: Observational Evidences For the Transmission Of Antibiotic Rmentioning

confidence: 99%

“…Enterobacteriaceae [59] Quinolone and fluoroquinolone Salmonella enterica [16] Escherichia coli [32] Sulfamethoxazole Salmonella enterica [16] Tetracycline Clostridium difficile [34] Multidrug Escherichia coli [33] Identification of the same genetic mobile element carrying the antibiotic resistance gene β-Lactam Escherichia coli [29,31,36,[60][61][62] Salmonella enterica [18,27,63] Colistin Escherichia coli [44][45][46][47][48][49][50][51] Salmonella enterica [64] Tetracycline Staphylococcus aureus [65] Environment and human Identification of the same antimicrobial-resistant strain β-Lactam Escherichia coli [35,41,66] Enterobacteriaceae [59] Multidrug Escherichia coli [40] Identification of the same genetic mobile element carrying the antibiotic resistance gene β-Lactam Enterobacteriaceae [67] Colistin Escherichia coli [53]…”

Section: Species Referencesmentioning

confidence: 99%

The need for efforts to obtain high quality evidence in a one health approach

Lee¹,

Lee²,

Park³

et al. 2018

biomedicalresearch

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The progressive increase of antibiotic resistance poses an alarming threat on public health worldwide. Although antibiotic resistance is a problem of human health, a one health approach to tackling antibiotic resistance is required, due to the effect of animal and environment on human health. To exactly estimate the impact of animal and environment on the spread of antibiotic resistance in human, scientific evidence with high quality is required. Molecular analysis of antibiotic-resistant bacteria through high-resolution, such as whole-genome sequencing, is suitable for obtaining credible evidence in examining the transmission of strains or genetic mobile elements responsible for antibiotic resistance among human, animal, and environment. In this paper, we discuss many observational studies using the whole-genome sequencing on various isolates collected from human, animal, and environment. These analyses suggest that more extensive surveillance based on whole-genome sequencing is required to accurately assess the potential threat of animal and environment on the spread of antibiotic resistance in human and that the effort to overcome various limitations in obtaining higher quality evidence is required.

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“…have been reported in healthcare institutions in Norway [ 4 – 6 ]. Yet another concern is that ESC-resistance often occurs along with co-resistance to important non-beta-lactam antibiotic classes [ 7 ], significantly limiting treatment options for these patients.…”

Section: Introductionmentioning

confidence: 99%

Travel to Asia is a strong predictor for carriage of cephalosporin resistant E. coli and Klebsiella spp. but does not explain everything; prevalence study at a Norwegian hospital 2014–2016

Espenhain

Jørgensen

Leegaard

et al. 2018

Antimicrob Resist Infect Control

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BackgroundWe aimed to estimate the prevalence of faecal carriage of extended-spectrum cephalosporin (ESC) resistant E. coli and K. pneumoniae (ESCr-EK) and vancomycin resistant enterococci (VRE) in patients upon hospital admission and identify factors associated with carriage to better target interventions and to guide empirical antibiotic treatment.MethodsBetween October 2014 and December 2016, we recruited patients admitted to a Norwegian university hospital. A rectal swab and questionnaire covering possible risk factors for colonisation were collected upon admission. Isolates were characterized by phenotypic methods. ESCr-EK isolates were subject to whole genome sequencing. We calculated prevalence and adjusted prevalence ratios (aPR) using binomial regression.ResultsOf 747 patients, 45 (6.0%) were colonised with ESCr-EK, none with VRE. The ESCr-EK isolates in 41 patients were multidrug resistant; no isolates were non-suceptible to meropenem. Prevalence of ESCr-EK was higher among travellers to Asia (aPR = 6.6; 95%CI 3.6–12; p < 0.001). No statistical significant difference in carriage was observed between departments, age or any other factors in the univariable analyses.ConclusionsThe observed prevalence of ESCr-EK colonisation upon admission was in the same range but lower than that reported in similar studies from Europe. Travel to Asia was a strong predictor for colonisation of ESCr-EK to be considered when administering empirical antimicrobial treatment. As less than one third of colonised patients had travelled to Asia, and no other factors investigated were found to be strongly associated with carriage, these findings underscore that healthcare personnel must apply standard infection control precautions for all patients.Electronic supplementary materialThe online version of this article (10.1186/s13756-018-0429-7) contains supplementary material, which is available to authorized users.

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A comparison of extended spectrum β-lactamase producing Escherichia coli from clinical, recreational water and wastewater samples associated in time and location

Cited by 75 publications

References 48 publications

ESBL-producing Escherichia coli in Swedish gulls—A case of environmental pollution from humans?

ESBL-producing Escherichia coli in Swedish gulls—A case of environmental pollution from humans?

The need for efforts to obtain high quality evidence in a one health approach

Travel to Asia is a strong predictor for carriage of cephalosporin resistant E. coli and Klebsiella spp. but does not explain everything; prevalence study at a Norwegian hospital 2014–2016

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