A Comparison of Greenhouse and Field Screening Methods for Blackleg Resistance in Doubled Haploid Lines ofBrassica napus

Bansal, V. K.; Kharbanda, P. D.; Stringam, G. R.; Thiagarajah, M. R.; Tewari, J. P.

doi:10.1094/pd-78-0276

Cited by 53 publications

(29 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, accurate evaluation of resistance to blackleg disease caused by the fungus Leptosphaeria maculans requires uniformly responding genotypes. Canola lines which consist of a mixture of genotypes because of a high level of cross pollination were shown to be unreliable in this respect (Bansal et al 1994). Furthermore, a lack of correlation between cotyledon or seedling resistance and adult plant resistance has often been observed (Salisbury and Ballinger 1993).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium tumefaciens-mediated Transformation of Double Haploid Canola (Brassica napus) Lines

Kazan¹,

Curtis²,

Goulter³

et al. 1997

Functional Plant Biol.

View full text Add to dashboard Cite

Double haploid (DH) genotypes of canola (Brassica napus L.) have a high level of genetic uniformity but have not been previously tested for genetic transformation. Transgenic plants from three of four DH genotypes derived from cv. Westar were obtained by inoculation of either hypocotyl segments or root explants with Agrobacterium tumefaciens. For hypocotyl transformation, A. tumefaciens strain LBA4404 containing a binary plasmid with the neomycin phosphotransferase gene (nptII) and a CaMV 35S-peroxidase gene cassette was co-cultivated with hypocotyl segments taken from the 5–6-day-old seedlings. Transformation frequencies for hypocotyl explants of two DH genotypes were 0.3–3%. Direct evidence for genetic transformation of hypocotyl explants was obtained through molecular hybridisation analysis. Using this protocol, mature transformed plants were obtained within 4–6 months of co-cultivation. A method of root transformation was successfully modified for one DH genotype of canola and transgenic plants were obtained at a frequency of 2%. Using this protocol, a peroxidase gene promoter–GUS fusion construct was introduced into a DH genotype. Tissue specific GUS expression driven by the peroxidase gene promoter in transgenic plants was analysed by GUS staining. Transformation systems for double haploid canola lines will permit the assessment of introduced genes for their effect on agronomic and physiological traits.

show abstract

Section: Introductionmentioning

confidence: 99%

Agrobacterium tumefaciens-mediated Transformation of Double Haploid Canola (Brassica napus) Lines

Kazan¹,

Curtis²,

Goulter³

et al. 1997

Functional Plant Biol.

View full text Add to dashboard Cite

show abstract

“…Typical CBB foliar symptoms that include wilting, drying, defoliation and apical die back were recorded. Disease incidence was calculated as described by Bansal et al [22] using the following formula:…”

Section: Assessment Of Pathogenicitymentioning

confidence: 99%

“…Disease severity (SD) was evaluated by the formula: SD (%) = Σ (number of diseased plants in a rating category × that rating category) / (total number of plants × category maximum score) × 100 [22]. The level of mortality was expressed as the percentage of plants completely dry compared to the total number of plants inoculated.…”

Section: Assessment Of Pathogenicitymentioning

confidence: 99%

Characterization of Congolese Strains of Xanthomonas axonopodis pv. manihotis Associated with Cassava Bacterial Blight

Mamba-Mbayi¹,

Tshilenge-Djim²,

Nkongolo³

et al. 2014

AJPS

View full text Add to dashboard Cite

Cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis has been reported in several African countries since 1970. Knowledge of the virulence and diversity of Xanthomonas axonopodis pv. manihotis strains is important for an integrated control of CBB. The main objective of the present study was to characterize strains of Xanthomonas axonopodis collected from various regions in the DR-Congo. There was variability among strains for shape (form), contour (margin) and elevation. Bacterial cell size for the strains analyzed varied from 24.1 µm × 11.3 µm to 11.4 µm × 4.2 µm. All the Xanthomonas axonopodis pv. manihotis strains but one was motile. Two distinctive groups were identified based on radial growth of their colonies. The first group grows faster (7.8 -10.5 mm/d) compared to the second group (4.8 -6.9 mm/d). Five strains (Gandajika, Inera/Stat, Kansasa, Mulumba and Musakatshi) were classified as virulent with a damage rating ≤ 1 and four were aggressive (Luputa, M'vuazi, Boketa and Kiyaka) with a damage rating ˃ 1. Significant differences were also observed among strains for disease onset, incidence and plant mortality. The highest incidence (33%) of bacterial blight 21 days after infestation (DAI) resulted from the Boketa strain inoculation and the lowest ( G. Mamba-Mbayi et al.

show abstract

“…These plants were discarded after rating them. A cotyledon test for blackleg resistance was done following Bansal et al (1994) on the same set of DH lines in a separate experiment. All DH lines along with parents were screened using a bulked mixture of eight single-spored virulent L. maculans isolates from collections made in the Canadian Prairie provinces (Alberta, Saskatchewan and Manitoba).…”

Section: Plant Materials and Crossesmentioning

confidence: 99%

“…All DH lines along with parents were screened using a bulked mixture of eight single-spored virulent L. maculans isolates from collections made in the Canadian Prairie provinces (Alberta, Saskatchewan and Manitoba). These isolates were chosen for their virulence and geographic distribution (Bansal et al, 1994). Thiagarajah et al (1999 suggested that glufosinate tolerance and blackleg resistance might be each under a monogenic control.…”

Section: Plant Materials and Crossesmentioning

confidence: 99%

Detecting and estimating segregation distortion and linkage between glufosinate tolerance and blackleg resistance in Brassica napus L.

et al. 2006

View full text Add to dashboard Cite

A Comparison of Greenhouse and Field Screening Methods for Blackleg Resistance in Doubled Haploid Lines ofBrassica napus

Cited by 53 publications

References 0 publications

Agrobacterium tumefaciens-mediated Transformation of Double Haploid Canola (Brassica napus) Lines

Agrobacterium tumefaciens-mediated Transformation of Double Haploid Canola (Brassica napus) Lines

Characterization of Congolese Strains of <i>Xanthomonas axonopodis</i> pv. <i>manihotis</i> Associated with Cassava Bacterial Blight

Detecting and estimating segregation distortion and linkage between glufosinate tolerance and blackleg resistance in Brassica napus L.

Contact Info

Product

Resources

About

A Comparison of Greenhouse and Field Screening Methods for Blackleg Resistance in Doubled Haploid Lines ofBrassica napus

Cited by 53 publications

References 0 publications

Agrobacterium tumefaciens-mediated Transformation of Double Haploid Canola (Brassica napus) Lines

Agrobacterium tumefaciens-mediated Transformation of Double Haploid Canola (Brassica napus) Lines

Characterization of Congolese Strains of &lt;i&gt;Xanthomonas axonopodis&lt;/i&gt; pv. &lt;i&gt;manihotis&lt;/i&gt; Associated with Cassava Bacterial Blight

Detecting and estimating segregation distortion and linkage between glufosinate tolerance and blackleg resistance in Brassica napus L.

Contact Info

Product

Resources

About

Characterization of Congolese Strains of <i>Xanthomonas axonopodis</i> pv. <i>manihotis</i> Associated with Cassava Bacterial Blight