Background: Pine flatwoods of the Southeast United States evolved with frequent wildfires. Land managers today mostly use prescribed burns to control fuels but also to restore historical lightning-caused wildfires dynamics. Though coarse outcomes of this practice are well-understood, fine scale impacts on many animal assemblages are still being explored. Frogs, for example, have upland and wetland requirements, limited mobility, and skin susceptible to desiccation and chemicals. Treefrogs, which are typically nontargeted for management, spend most of their lives in uplands away from water. When fire approaches, these animals will escape to an unburned area, hide in place, or die. Results: We assessed impacts of prescribed fire on the dynamics of an upland flatwoods specialist, the pinewoods treefrog Dryophytes femoralis using a replicated before-after-control-impact field experiment. Using single rope technique and climbing equipment, we set PVC pipes as treefrog refugia at 3m, 6m, 9m, and 9+m in Longleaf Pines, Pinus palustris (n=12 tress). Three trees were selected in each patch per treatment: two unburned and two burned patches during the 2021 burning season (n=4 patches). Every two weeks between March and September we checked pipes for frogs and assigned them unique Visible Implant Elastomer (VIE) marks. We obtained data from 76 individuals with an additional 198 recaptures. Using mark-recapture methods and mixed linear models with Bayesian framework, we modeled abundance, survival, and vertical movement. Survival and recapture were comparable among treatments, but abundance, new frogs and probability of moving up or down a tree varied. Frogs in trees in burned units were more likely to stay in place and less likely to descend to lower heights. We observed an increased probability of finding new frogs and more abundance in trees after a fire compared to unburned controls. Conclusions: We found the prevailing mechanism for resiliency to fire for pinewoods treefrogs was migration up large pines, then recolonization of the shrub layer when it regreens post-fire. This finding substantiates conclusions from other works that integrity of mature pines is key to sustaining native biodiversity. Future work and management should consider the three-dimensional structure of habitat when developing burn prescriptions and study designs.