A comparison of Hordeum bulbosum-mediated haploid production efficiency in barley using in vitro floret and tiller culture

Abstract

A high efficiency of Hordeum bulbosum-mediated haploid production in barley has been achieved using a floret culture technique in which florets pollinated with Hordeum bulbosum are cultured on modified N6 media containing 0.5 mg/l kinetin and 1.2 mg/l2,4-D. Cultures were maintained at 25 °C with a 16 h photoperiod for 9 days before embryo rescue. In a comparison of haploid production efficiency using five F1 hybrids from winter x winter and winter x spring barley crosses, 41.6 haploid plants/100 florets pollinated were produced using floret culture. Using detached tiller culture, 13.5 haploid plants/100 florets pollinated were produced. Higher efficiencies achieved with floret culture are attributed to the formation of larger, differentiated embryos. Such embryos lead to higher frequencies of plant regeneration. The F1 from a winter x winter cross was inferior in haploid production compared to F1s from winter x spring crosses. No genotype x technique interaction was observed.

A high-density transcript linkage map of barley derived from a single population

“…The number of missing data points per locus, as well as the segregation values for each locus, are shown in Supplementary Table 1. Replacing missing data points will slightly improve map quality, whereas the unidirectional segregation distortion of alleles at each cluster suggests a biological basis, perhaps related to gametophytic selection for performance in microspore culture, which tends to produce more lethal haploids compared with the haploid induction by Hordeum bulbosum (Chen and Hayes, 1989). Sayed et al (2002) and Muñ ozAmatriain et al (2008) reported distorted marker segregation in anther culture-derived mapping populations.…”

“…Cytogenetics subsequently contributed to the genetic mapping by physically localizing genes (Kü nzel et al, 2000). Genetic mapping of barley accelerated with the application of molecular markers to doubled haploid (DH) populations (Chen and Hayes, 1989). These DH populations have the added advantage of allowing for simultaneous genetic map construction and repeated analysis of quantitative traits .…”

QTL analysis of agronomic traits in barley based on the doubled haploid progeny of two elite North American varieties representing different germplasm groups

“…One hundred and forty doubled haploid (DH) lines were produced from the F 1 of Harrington×Morex by the Hordeum bulbosum method (Chen and Hayes 1989). One hundred and six markers were used for construction of a base map, with a target density of 10 cM (Hayes et al 1997).…”

Suppression of the Barleyuroporphyrinogen III synthaseGene by aDsActivation Tagging Element Generates Developmental Photosensitivity

“…The barley Uros gene was mapped using 121 ApaI-digested progeny DNAs from a Steptoe X Morex doubled haploid family (Chen and Hayes, 1989). BAC clone 0031J02 was isolated by screening arrayed BAC clone DNA filters from a barley cultivar Morex genomic library (HvMBa) obtained from the Clemson University Genomic Institute.…”