A comparison of ion shifts with adenosine triphosphate and creatine phosphate levels in muscle

Briner, G.P.; Simon, Shirley E.; Frater, R.; Tasker, Patricia

doi:10.1016/0006-3002(59)90398-1

Cited by 20 publications

(5 citation statements)

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“…It cannot be stated with certainty whether this lactic acid production was due to the vasoconstriction which followed the potassium depolarization or whether is was due to other causes. Among these a reduction of intracellular ATP concentration could be the essential mechanism (due to uncoupling of oxidative phosphorylation) . Briner, Simon, Frater and Tasker (1959) found a reduced ATP content in muscle soaked in Ringer solutions with 20 mM potassium.…”

Section: Discussionmentioning

confidence: 99%

Glucose Uptake in Potassium‐Depolarized Mammalian Muscle

Crone

1966

Acta Physiologica Scandinavica

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CRONE, C. Glucose uptake in potassium-depolarized mammalian muscle. Acta physiol. scand. 1966. 68. 105-1 17.Experiments were performed on perfused cat hind limbs to study whether the uptake of glucose by striated muscle is linked with active transport of ions. The glucose uptake in the control periods was 1.4 pmole/100 g/min. Elevation of the plasma potassium concentration to 25-40 meq/l led to an average glucose uptake of 3.0 pmoles/100 g/min. Addition of strophanthin to a final concentra-105 106 CHRISTIAN CRONE

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Section: Discussionmentioning

confidence: 99%

Glucose Uptake in Potassium‐Depolarized Mammalian Muscle

Crone

1966

Acta Physiologica Scandinavica

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“…The effects of external K, for the range of concentrations considered in this paper, on oxygen consumption, heat production, glycolysis, and organophosphoryl levels in frog muscle have been extensively studied (4,9,10,21,23,26,28,29). Hegnauer et al (9), in an early and important paper, showed that, among other metabolic effects, the oxygen consumption was increased when the [K]o is increased.…”

Section: Mechanism Of the K-stimulated Na Effluxmentioning

confidence: 99%

“…What is significant for the discussion here is that heat production is increased sufficiently and at precisely the same K concentrations as give rise to the increased Na efflux. Many theories have been advanced to explain the increased metabolic rates produced by K ions (4,10,21,23,26,28). The data presented in this and the following paper provide support for the idea originally advanced by Hill and Howarth (10) that the effects on the metabolism can be ascribed to the depolarizations produced by the increased [K] o.…”

Section: Mechanism Of the K-stimulated Na Effluxmentioning

confidence: 99%

“…With precautions to ensure identical positioning of planchets with respect to the auxiliary detector, the known sample of Na 24 was counted and the sensitivity, S., was calculated in units of mole Na per count/minute. The sensitivity of the experimental detector, S,, was calculated from the formula, S, = rS, (4) where r was the ratio of the bundle-counting rate with the auxiliary detector to the bundle-counting rate with the experimental detector at the time when the fibers were removed from the observation cell. The average influx of sodium per unit length of muscle fiber was calculated from the formula where6 N…”

Section: Flux Measurementsmentioning

confidence: 99%

“…5). In the Na" 4 Ringer's solution with strophanthidin, the fibers picked up an additional 263 counts/minute of Na". A final control influx of Na 24 , period C, after 90 minutes in inactive Ringer's solution gave an increment of 270 counts/minute.…”

Section: Effects Of Strophanthidin On Resting Sodium Fluxesmentioning

confidence: 99%

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Effects of External Potassium and Strophanthidin on Sodium Fluxes in Frog Striated Muscle

Horowicz

Gerber

1965

The Journal of General Physiology

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Unidirectional Na fluxes in isolated fibers from the frog's semitendinosus muscle were measured in the presence of strophanthidin and increased external potassium ion concentrations. Strophanthidin at a concentration of 10 -5 M inhibited about 80 per cent of the resting Na efflux without having any detectable effect on the resting Na influx. From this it is concluded that the major portion of the resting Na efflux is caused by active transport processes. External potassium concentrations from 2.5 to 7.5 mM had little effect on resting Na efflux. Above 7.5 mM and up to 15 mM external K, the Na efflux was markedly stimulated; with 15 mM K the Na influx was 250 to 300 per cent greater than normal. On the other hand, Na influx was unchanged with 15 mM K. The stimulated Na efflux with the higher concentrations was not appreciably reduced when choline or Li was substituted for external Na, but was completely inhibited by 10 -5 M strophanthidin. From these findings it is concluded that the active transport of Na is stimulated by the higher concentrations of K. It is postulated that this effect on the Na "pump" is produced as a result of the depolarization of the muscle membranes and is related to the increased metabolism and heat production found under conditions of high external K.

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Possible role of Ca ions in the resting metabolism of frog sartorius muscle during potassium depolarization

Νovotný

Vyskočil

1966

Journal Cellular Physiology

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Oxygen consumption and Ca exchangeability at different levels of potassium depolarization were studied in frog sartorius muscle. It was found that the changes in oxygen consumption parallel the changes in Ca exchangeability. Procaine M) and CaClz (2.10-2 M ) suppressed both extra oxygen consumption and Ca exchangeability at low values of depolarization. At higher values of depolarization procaine and CaCls differed in their action. Procaine favored inhibition of these processes, CaCL caused their activation. The effects of these compounds was not a result of a change in the membrane potential, since their effect on potassium depolarization was found to be small. Relations between oxygen consumption and Ca exchangeability similar to those observed at potassium depolarization seem to exist under conditions where caffeine was applied. It is proposed that the extra oxygen consumption caused by potassium depolarization or on application of caffeine and unaccompanied by mechanical changes is related to the release of Ca from its bound form. Oxygen consumption in isotonic sucrose solution was also studied, but some different data from the above were obtained.The effect of potassium depolarization on frog muscle has been the object of physiological studies for several decades. However, a detailed picture of electrical and mechanical changes in fast muscle fibers during potassium depolarization has only recently emerged from the studies of Adrian ('56) and Hodgkin and Horowicz ('60). Their results offered also a new basis for studies on relations between electrical and mechanical processes in frog muscle fibers on one hand and metabolic processes on the other hand. The exact mechanism of the relations between these processes in frog muscle is not known so far. Nevertheless, on the basis of the above mentioned studies and the works of other authors (Hegnauer, Fenn and Cobb, '34; Solandt, '36; Hill and Howarth, '57; Briner et al., '59; Muller and Simon, '60; dealing with metabolism in frog sartorius, the processes taking place in this muscle during potassium depolarization may be described as follows: If the membrane potential of frog sartorius fibers is reduced by high potassium depolarization to values lower than 50 mV, phasic contractures and a short-time increase in metabolism occur. If, however, the membrane potential is reduced only to values higher than 50 mV, a J. CELL. PHYSIOL., 67: 159-168.long-lasting increase in metabolism takes place without any shortening of the muscle. In sartorius of the frog Rana temporaria, a ten-fold increase in oxygen consumption is observed at the decrease of the membrane potential to values of about 55 mV.This increase in metabolism is unaccompanied by mechanical changes and has been an unexplained problem for a long time. Recently it was discussed at large by Hill and Howarth ('57) and it was pointed out that the extra metabolism during potassium depolarization cannot be connected with processes governing ionic equilibria between the muscle fibers and the extracellular space. ...

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A comparison of ion shifts with adenosine triphosphate and creatine phosphate levels in muscle

Cited by 20 publications

References 19 publications

Glucose Uptake in Potassium‐Depolarized Mammalian Muscle

Glucose Uptake in Potassium‐Depolarized Mammalian Muscle

Effects of External Potassium and Strophanthidin on Sodium Fluxes in Frog Striated Muscle

Possible role of Ca ions in the resting metabolism of frog sartorius muscle during potassium depolarization

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