A Comparison of Ki67, Syndecan-1 (CD138), and Molecular RANK, RANKL, and OPG Triad Expression in Odontogenic Keratocyts, Unicystic Ameloblastoma, and Dentigerous Cysts

Brito-Mendoza, Luisana; Bologna‐Molina, Ronell; Irigoyen-Camacho, María Esther; Martínez, Guillermo Ramírez; Sánchez‐Romero, Celeste; Mosqueda‐Taylor, Adalberto

doi:10.1155/2018/7048531

Cited by 17 publications

(33 citation statements)

References 21 publications

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“…The results are partially different from those of Al-Otaibi et al, and different from those of Al-Otaibi et al [ 38 , 41 ]. Brito-Mendoza et al found no significant difference between OKC and DC but found the mean rank scores of AB to be significantly lower than those of OKC and DC [ 38 ].…”

Section: Discussioncontrasting

confidence: 96%

“…It is worthwhile to mention here that Nadalin et al observed low or absent expression of syndecan-1 in areas of OKC lining in which there was an alteration in epithelial morphology due to inflammation [37]. e distribution patterns of syndecan-1 in the epithelia of DC, OKC, and AB in this study were consistent with the findings of other studies [37,38,40,41]. ey were, however, unlike those ofÖzcan et al, who reported a different distribution in the OKC, describing it as a strong membranous expression in basal and suprabasal cells only, while, as in this study, it involved the full thickness of DC lining [39].…”

Section: Discussionsupporting

confidence: 91%

“…Some biologic markers have not been investigated in the OKC yet, such as mammary serine protease inhibitor (maspin). Other markers were investigated only in a few studies, such as syndecan-1 [37][38][39][40][41].…”

Section: Introductionmentioning

confidence: 99%

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Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis

Hammad

Nagrash

Safadi

2020

International Journal of Dentistry

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The odontogenic keratocyst (OKC) is a controversial lesion that was reclassified as a tumor with the name “keratocystic odontogenic tumor” in 2005. The reclassification was revoked recently in 2017, with a conclusion on the need for further studies on the subject. In this study, the expressions of an important regulatory protein (maspin), an important integral membrane proteoglycan (syndecan-1), and a universal proliferation marker (Ki-67) in the epithelium of the OKC were investigated in comparison with the dentigerous cyst (DC) and ameloblastoma (AB). Twenty-six OKCs, eleven DCs, and ten conventional ABs were immunohistochemically stained for maspin, syndecan-1, and Ki-67. ImageJ was used to analyze the positivity of maspin and syndecan-1. The Ki-67 score was calculated as the percentage of positive nuclei in 5 high power fields. Analysis of variance (ANOVA) test and Student t-test were used as appropriate. Lower expressions of maspin were noted in OKC and DC compared to those in AB, and lower expressions of syndecan-1 were noted in OKC and AB compared to those in DC. The differences, however, did not reach statistical significance (ANOVA and t-test: P>0.05). The Ki-67 score was significantly higher in OKC than in DC (t-test: P<0.05), and not significantly different from AB (t-test: P>0.05). In conclusion, expressions of maspin and syndecan-1 are not strongly representative of differences in behavior between OKC, AB, and DC. However, the expression of Ki-67 indicates comparable proliferative activities of OKC and AB, which are higher than that of DC. Further investigation on the biologic behavior of OKC is still recommended to arrive at more specific conclusions regarding its classification.

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Section: Discussioncontrasting

confidence: 96%

Section: Discussionsupporting

confidence: 91%

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Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis

Hammad

Nagrash

Safadi

2020

International Journal of Dentistry

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“…The nuclear protein, Ki-67 antigen is a reliable marker reflecting cell proliferation, and Ki-67 is more specific for the proliferation of ameloblastoma and AC [ 13 ]. In this study, immunohistochemistry revealed that the proliferation index of Ki-67 in secondary tumors was higher than that in primary tumors, but radiography revealed that primary tumors were more destructive than secondary tumors, indicating that the increase in the Ki-67 index could not explain the invasiveness and bone destruction of those lesions but could help explain its ability to sustain growth and expansion [ 14 ]. Therefore, using the Ki-67 index increase to illustrate the destructive ability of AC remains a subjective measure [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Study on clinical and biological characteristics of ameloblastic carcinoma

Niu

Chen

et al. 2020

Orphanet J Rare Dis

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Background Ameloblastic carcinoma (AC) is an odontogenic malignant tumor which is closely related to benign ameloblastoma. Because of its rarity, diagnosis and treatment are difficult. In this study, we summarized and analyzed the clinical and biological characteristics of AC. Results Fifteen patients with AC and a median age of 53 years were identified. Among of them, five patients who were tested carried a BRAF-V600E mutation. Two patients presented with cervical lymph nodes and lung metastases. Primary AC was more invasive, and the bone destruction ability of the primary type was more radical than that of the secondary type. Conclusions This study revealed that the BRAF-V600E mutation was related to the aggressive behavior of AC, and early radical resection is crucial. Moreover, targeted therapy may be a new direction in the future.

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“…RANK expression was higher in OKC and OPG expression was low, without differences between lesions. Brito et al (2018) evaluated the expression of the RANK/RANKL/OPG triad in unicystic ameloblastoma, OKC, and DC [27]. Higher expression of RANKL was observed in unicystic ameloblastoma, followed by OKC.…”

Section: Analysis Of the Immunoexpression Of Osteoclastogenic Factorsmentioning

confidence: 99%

Differential Protein Expression of Osteoclastogenic Factors in Odontogenic Cysts and Tumors

Almeida

Leite

Almeida

et al. 2020

Preprint

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Background: The osteolytic activity of odontogenic cysts and tumors is directly associated with their growth and aggressiveness. Proteins expressed by epithelial and mesenchymal cells can influence this biological event differently in indolent cystic lesions, aggressive cystic lesions, and odontogenic tumors.Methods: The objective of this study was to compare the immunohistochemical expression of factors that stimulate RANKL (Receptor activator of nuclear factor kappa-Β ligand), CatK (cathepsin K) and MMP8 (Matrix Metallopeptidase 8) and inhibit OPG (osteoprotegerin) osteoclastogenesis between dentigerous cyst (DC), glandular odontogenic cyst (GOC), odontogenic keratocyst (OKC), and ameloblastoma (AB). Paraffin-embedded sections of 9 DCs, 9 GOCs, 20 OKCs, 21 ABs, and 4 dental follicles (DFs) were submitted to immunohistochemistry. The immunoreactivity in epithelium and connective tissue was analyzed semi-quantitatively and quantitatively, respectively. Immunoexpression of the proteins was observed in epithelial and mesenchymal cells of all lesions studied.Results: The expression of RANKL and CatK was higher in OKC, AB, and GOC (p<0.005). Higher expression of OPG was found in DF and DC compared to the other markers (p<0.005). MMP8 expression was high in GOC e OKC.Conclusions: This study demonstrated the differential expression of factors that inhibit and stimulate bone resorption during the development of DC, GOC, OKC and AB. Higher expression of RANKL and CatK was observed in more aggressive lesions. OPG appears to be one of the molecules responsible for the slower growth of DC.

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A Comparison of Ki67, Syndecan-1 (CD138), and Molecular RANK, RANKL, and OPG Triad Expression in Odontogenic Keratocyts, Unicystic Ameloblastoma, and Dentigerous Cysts

Cited by 17 publications

References 21 publications

Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis

Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis

Study on clinical and biological characteristics of ameloblastic carcinoma

Differential Protein Expression of Osteoclastogenic Factors in Odontogenic Cysts and Tumors

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