A Comparison of Manual and Automatic Ultrasound‐guided Biopsy Techniques

Hoppe, F.; Hager, David; Poulos, Paul W.; Ekman, Stina; Lindgren, P. G.

doi:10.1111/j.1740-8261.1986.tb00012.x

Cited by 40 publications

(27 citation statements)

References 3 publications

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“…In our perspective, one of the main factors that contributed to the success of the biopsies was the semi-automatic mechanism of the needle cannula. This system reduced the need to repeat the technique to assure the recovery of larger samples than those obtained with the manual Tru-Cut needles (Hoope et al, 1986).…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of laparoscopic and ultrasound-guided biopsy methods for gene expression analysis in transgenic goats

et al. 2015

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ABSTRACT. The present study aimed to compare laparoscopic (LP) and ultrasound-guided (US) biopsy methods to obtain either liver or splenic tissue samples for ectopic gene expression analysis in transgenic goats. Tissue samples were collected from human granulocyte colony stimulating factor (hG-CSF)-transgenic bucks and submitted to realtime PCR for the endogenous genes (Sp1, Baff, and Gapdh) and the transgene (hG-CSF). Both LP and US biopsy methods were successful in obtaining liver and splenic samples that could be analyzed by PCR (i.e., sufficient sample sizes and RNA yield were obtained). Although the number of attempts made to obtain the tissue samples was similar (P > 0.05), LP procedures took considerably longer than the US method (P = 0.03). Finally, transgene transcripts were not detected in spleen or liver 8673 Biopsy methods for ectopic expression in transgenic bucks ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 8672-8684 (2015) samples. Thus, for the phenotypic characterization of a transgenic goat line, investigation of ectopic gene expression can be made successfully by LP or US biopsy, avoiding the traditional approach of euthanasia.

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Section: Discussionmentioning

confidence: 99%

Comparative analysis of laparoscopic and ultrasound-guided biopsy methods for gene expression analysis in transgenic goats

et al. 2015

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“…Já o uso das agulhas Tru-cut foi associado às melhores taxas de recuperação das amostras, com médias de tentativas e desvios padrões menores. Além da relatada eficiência do método de guilhotina (Buckley et al 1986), outro fator que parece ter contribuído para este melhor resultado foi o mecanismo de avanço semi-automático da cânula da agulha, que reduz a necessidade de repetição da técnica por garantir a recuperação de amostras maiores do que as obtidas com agulhas Tru-cut manuais (Hoope et al 1986). Diferente do que ocorreu com as agulhas Menghini, a reutilização não afetou o funcionamento das agulhas Tru-cut, fato já apontado em trabalho correspondente (Kerwin 1995).…”

Section: Avaliação Das Amostrasunclassified

Avaliação de técnicas de biópsia hepática em ovinos

et al. 2010

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RESUMO.-Devido à carência de informações sobre o tema e à ausência de estudos que confrontem as técni-cas de biópsia hepática em ovinos, foi desenvolvido um estudo comparativo entre oito técnicas de biópsia nesta espécie. Neste estudo foram utilizadas oito borregas (17,95kg ± 2,71) mestiças (Santa Inês) em procedimentos seriados por oito semanas consecutivas e avaliados os aspectos clínicos, os achados de patologia clínica, o peso das amostras hepáticas, a qualidade histológica, o número de veias centrolobulares e espaços-porta e a presença de artefatos no corte histológico de amostras hepáticas obtidas através de seis técnicas percutâneas e duas técnicas videolaparoscópicas. Para as técnicas percutâneas empregaram-se dois tipos de agulhas, Men- Due to lack of information about the subject and lack of studies that confront the techniques of liver biopsy in sheep, a comparative study between eight biopsy techniques in this species was developed. In this study, eight crossbred (Santa Inês) ewe lambs (17.95 kg ± 2.71) were used in serial procedures for eight consecutive weeks. Liver samples obtained through six percutaneous techniques and two videolaparoscopic techniques were analyzed. The clinical aspects, clinical pathological findings, hepatic sample weights, histology quality, number of centrilobular veins and portal spaces, and the presence of artifacts in the histology slices were recorded. For the percutaneous techniques, two types of needles were used, modified Menghini (M) and semi-automatic tru-cut (T). They were used in combinations with blind biopsies, guided by ultrasound (US) and monitored by video laparoscopy (VL) biopsies. In the videolaparoscopy procedures, Blakesley biopsy forceps and the method of liver fragment resection by VL were used. No relevant hematological or biochemical alterations were found and the clinical manifestations were slight and transitory. In a general way, the use of US and VL in percutaneous biopsies did not yield a significant improvement in weight and histology quality of the liver samples; however, the videolaparoscopic techniques allowed a wider access to the liver than the percutaneous techniques. The quality of the samples obtained through both VL techniques were equivalent; however, the resection technique enabled the collection of heavier samples, but determined the formation of perihepatic adherences. More difficulty in fragment retrieval, more fluctuation in weight and less quality were found in samples obtained with M needles when compared to T needles.INDEX TERMS: Liver biopsy, videolaparoscopy, ultrasonography, sheep.

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“…Todas as técnicas propiciaram a obtenção de amostras com quantidade de glomérulos superior àquela estimada como necessária para avaliações histopatológicas (Quadro 3) (Hoppe et al 1986, Wise et al 1989, LauferAmorim et al 2002. Entre estas estimativas descritas em estudos de medicina humana, considera-se que a representatividade das amostras renais depende de pelo menos quatro ou sete glomérulos e que o aumento deste número não resultaria em maior eficácia diagnóstica da amostra (Laufer-Amorim et al 2002).…”

Section: Complicações Causadas Pelas Técnicasunclassified

Avaliação de técnicas de biópsia renal em ovinos

et al. 2010

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ABSTRACT.-Néspoli P.B., Gheller V.A., Peixoto P.V., Carvalho A.U., França T.N., Facury Filho E.J., Godoy Due to lack of studies about renal biopsies in sheep, a comparative study was performed for three renal biopsy techniques in this species. In this study, nine crossbred (Santa Inês) ewe lamb (26.64 kg ±4,86) were used in serial procedures with one week consecutive intervals. The clinical aspects, clinical pathological findings, renal sample weights, histology quality, number of glomeruli, and the presence of artifacts in the histology slices were evaluated using the techniques of percutaneous blind biopsy, ultrasound guided biopsies and of videolaparoscopic procedure. No relevant hematological or biochemical alterations were observed in the animals subjected to renal biopsies and the clinical manifestations detected were slight and transitory, except for one case of urethral obstruction by blood clot. The blind technique was related to more frequent and severe cases of hematuria, with more severe damage to the renal tissue and to the only case of obstruction of the urinary flow. In the videolaparocopic technique, the average weight of the samples was superior and hematuria was slight and transitory. A direct relation was seen between occurrence of severe hematuria and presence of transitional epithelium in the samples and the number of trials used for obtainment of fragments. INDEX TERMS: Renal biopsy, videolaparoscopy, ultrasonography, sheep.

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A Comparison of Manual and Automatic Ultrasound‐guided Biopsy Techniques

Cited by 40 publications

References 3 publications

Comparative analysis of laparoscopic and ultrasound-guided biopsy methods for gene expression analysis in transgenic goats

Comparative analysis of laparoscopic and ultrasound-guided biopsy methods for gene expression analysis in transgenic goats

Avaliação de técnicas de biópsia hepática em ovinos

Avaliação de técnicas de biópsia renal em ovinos

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