A comparison of melatonin and α-lipoic acid in the induction of antioxidant defences in L6 rat skeletal muscle cells

Favero, Gaia; Rodella, Luigi Fabrizio; Nardo, Lorenzo; Giugno, Lorena; Cocchi, Marco Angelo; Borsani, Elisa; Reíter, Russel J.

doi:10.1007/s11357-015-9824-7

Cited by 23 publications

(25 citation statements)

References 54 publications

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“…Furthermore, in our study, we evaluated the effects of melatonin treatment on skeletal muscle cell atrophy induced by reserpine observing that melatonin causes a significant increment in muscle weight and in myotubes diameter compared with RIM experimental group and the animals treated with reserpine and then only with tap water. In addition previous studies reported that melatonin reduces skeletal muscle atrophy and oxidative stress [ 49 , 61 , 62 ]. In order to confirm the antioxidant, ant-inflammatory and protective roles of melatonin, we evaluated the skeletal muscle expression of endogenous antioxidant and of constitutive molecules and we found that melatonin modulates muscoloskletal ultrastructure, increases expression of SOD1, CAT, COX-1 and SIRT3 and decreaes NLRP3 compared with RIM experimental group and also with the groups treated reserpine and then only with tap water.…”

Section: Discussionmentioning

confidence: 98%

Oral Supplementation of Melatonin Protects against Fibromyalgia-Related Skeletal Muscle Alterations in Reserpine-Induced Myalgia Rats

Favero

Trapletti

Bonomini

et al. 2017

IJMS

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Fibromyalgia is a chronic syndrome characterized by widespread musculoskeletal pain and an extensive array of other symptoms including disordered sleep, fatigue, depression and anxiety. Important factors involved in the pathogenic process of fibromyalgia are inflammation and oxidative stress, suggesting that ant-inflammatory and/or antioxidant supplementation might be effective in the management and modulation of this syndrome. Recent evidence suggests that melatonin may be suitable for this purpose due to its well known ant-inflammatory, antioxidant and analgesic effects. Thus, in the current study, the effects of the oral supplementation of melatonin against fibromyalgia-related skeletal muscle alterations were evaluated. In detail, 90 Sprague Dawley rats were randomly treated with reserpine, to reproduce the pathogenic process of fibromyalgia and thereafter they received melatonin. The animals treated with reserpine showed moderate alterations at hind limb skeletal muscles level and had difficulty in moving, together with significant morphological and ultrastructural alterations and expression of inflammatory and oxidative stress markers in the gastrocnemius muscle. Interestingly, melatonin, dose and/or time dependently, reduced the difficulties in spontaneous motor activity and the musculoskeletal morphostructural, inflammatory, and oxidative stress alterations. This study suggests that melatonin in vivo may be an effective tool in the management of fibromyalgia-related musculoskeletal morphofunctional damage.

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Section: Discussionmentioning

confidence: 98%

Oral Supplementation of Melatonin Protects against Fibromyalgia-Related Skeletal Muscle Alterations in Reserpine-Induced Myalgia Rats

Favero

Trapletti

Bonomini

et al. 2017

IJMS

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“…Remarkably, melatonin supplementation in NLRP3 KO mice was particularly beneficial to retard the onset of sarcopenia in gastrocnemius muscle in aged animals [91]. Interestingly, exogenous melatonin regulated insulin resistance, ameliorated mitochondrial function in rat muscles, and prevented chemically induced apoptosis and endoplasmic reticulum stress in different skeletal muscle cells in vitro [92][93][94][95][96][97].…”

Section: Melatonin Alleviates Skeletal Muscle Disorders In Vitro and mentioning

confidence: 99%

Impact of Melatonin on Skeletal Muscle and Exercise

Stacchiotti

Favero

Rodella

2020

Cells

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Skeletal muscle disorders are dramatically increasing with human aging with enormous sanitary costs and impact on the quality of life. Preventive and therapeutic tools to limit onset and progression of muscle frailty include nutrition and physical training. Melatonin, the indole produced at nighttime in pineal and extra-pineal sites in mammalians, has recognized anti-aging, anti-inflammatory, and anti-oxidant properties. Mitochondria are the favorite target of melatonin, which maintains them efficiently, scavenging free radicals and reducing oxidative damage. Here, we discuss the most recent evidence of dietary melatonin efficacy in age-related skeletal muscle disorders in cellular, preclinical, and clinical studies. Furthermore, we analyze the emerging impact of melatonin on physical activity. Finally, we consider the newest evidence of the gut–muscle axis and the influence of exercise and probably melatonin on the microbiota. In our opinion, this review reinforces the relevance of melatonin as a safe nutraceutical that limits skeletal muscle frailty and prolongs physical performance.

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“…Notably, conditional ablation of the pancreatic clock causes diabetes mellitus (DM) due to impaired beta-cell function at the very latest stage of stimulus-secretion coupling. These results demonstrate a role of the beta-cell clock in coordinating insulin secretion with the sleep-wake cycle and reveal that ablation of the pancreatic clock can trigger the DM onset [4].…”

Section: Introductionmentioning

confidence: 62%

“…Oxidative stress plays a pivotal role in the development of diabetes complications, both microvascular and cardiovascular. Melatonin [4,12], a potent antioxidant agent, is essential for glucose homeostasis and regulation. It has been determined (Czuczejko J., Sielski Ł., 2019) that melatonin supplementation influenses oxidative stress parameters in elderly patients with noninsulin dependent diabetes mellitus.…”

Section: Resultsmentioning

confidence: 99%

The Effect of Melatonin on the Energy Metabolism in Heart of Diabetic Rats

Kushnir

Yaremii

2019

Act. Probl. of the Modern Med.

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The aim of this study was to determine the effect of melatonin on activity of pyruvate kinase (PK) and lactate dehydrogenase (LDH) in the heart tissue and on the activity of succinate dehydrogenase (SDG) and H+-ATPase in mitochondria of cardiomyocytes of rats with dexamethasone diabetes. Methods. The research was performed in compliance with the Rules of the work with test animals (1977) and the European Convention on the Protection of Vertebrate Animals used in experiments and other scientific purposes (18 March 1986). The experiments were carried out on 36 sexually mature male albino rats with the body mass 0.18 – 0.20 kg. The animals were divided into 3 groups:1) control group; 2) group with diabetes mellitus DM (BG level ≥ 8.0 mmol/l); 3) diabetic animals which were injected with melatonin. Melatonin (“Sigma”, USA) was injected intraperitoneally in a dose of 10 mg/kg of body weight daily over 13 days. Dexamethasone diabetes was evoked by daily subcutaneous administration of dexamethasone (solution for injection 4 mg / ml, KRKA, Slovenia) at a dose of 0.125 mg / kg body weight for 13 days (O.V. Stefanov, 2001). The mathematical model of the insulin-glucose correlation by the Homeostasis Model Assessment (HOMA) was used to evaluate the insulin resistance in the animals (D.R.Matthews et al., 1985). The blood was taken from the tail vein to evaluate the BG level using “OneTouchUltra” (“LifeScan”, USA). The rats were sacrificed on the 14th day of the experiments in accordance with the ethical treatment of animals. 5% homogenate was prepared from cold-isolated rat hearts on chilled 50 mM Tris-HCl buffer (pH = 7.4) with sucrose. Mitochondria were isolated by differential centrifugation. Enzyme activities in the mitochondrial fraction was determined by: succinate dehydrogenase (SDG) according to the method of Eshchenko N.D. et al. (1992), H + -ATP-ase - for Gabibiv M.M. (1986). Serum insulin content was determined using an automatic immunochemiluminescent analyzer (Snibe Co., Ltd, PRC) using a Maglumi test kit. The insulin resistance index (HOMA-IR) was calculated by the formula: HOMA-IR = (glucose (mmol / l) • insulin (mcU / ml)) / 22.5. To determine the enzymes activities by standart methods, heart muscle tissue was quickly removed, rinsed in saline, blotted, weighed and homogenized. Then the homogenate (5% in ice-cold 0.25 mM tris-HCl-buffer) was ultracentrifugated (10 min at 1500r/min) and the supernatant was used for measurements. Statistical analysis was performed using Statistica 10 (StatSoft Inc). Prior to analysis, Shapiro-Wilk test was used to assess the normality of the group data. According to the criterion, the samples distributions differed from normal distribution. Given these, use of the Mann-Whitney test was considered sufficient for valid conclusions to be made. Differences were considered to be statistically significant when P ≤ 0.05. Results. In the blood of all diabetic rats on the 14th day the glucose content exceeded 8.9 mmol / l, and the HOMA-IR index increased 7 times. According to results, we have obtained the activity of PK in the heart tissue of diabetic animals was 55% less than in control group. It can be explained by low uptake of glucose from the blood by heart muscle tissue in conditions of insulin resistance. Reduced glucose uptake by peripheral tissues, in turn, leads to a reduced rate of glucose metabolism. That means now substrate for next catabolic changes in glycolysis. The activity of LDH increases by 32% in the heart muscles of diabetic rats compared with the control values. We have found out that the activity of PK and LDH has become normalized by the introducing of melatonin in dose of 10 mg/kg of body weight. In the mitochondria of the animals with dexamethasone diabetes, the activity of SDG and H+ -ATPase is significantly lower than that in intact animals by 45 and 32%, respectively. Probably, melatonin enhances the uptake of glucose by heart muscles. The blood glucose content of diabetic rats, which were administered is not significantly different from that of the intact animals, and the HOMA-IR was 6.3 times lower than that of diabetic rats. The activity of SDG and H+-ATPase in the mitochondria of rats receiving both melatonin and dexamethasone does not differ from that of intact rats. Conclusion. A 14-day introduction of melatonin leads to a marked improvement of the state of carbohydrate metabolism as well as the energy metabolism in heart tissue that is accompanied by the normalization of the indices studied.

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A comparison of melatonin and α-lipoic acid in the induction of antioxidant defences in L6 rat skeletal muscle cells

Cited by 23 publications

References 54 publications

Oral Supplementation of Melatonin Protects against Fibromyalgia-Related Skeletal Muscle Alterations in Reserpine-Induced Myalgia Rats

Oral Supplementation of Melatonin Protects against Fibromyalgia-Related Skeletal Muscle Alterations in Reserpine-Induced Myalgia Rats

Impact of Melatonin on Skeletal Muscle and Exercise

The Effect of Melatonin on the Energy Metabolism in Heart of Diabetic Rats

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