A comparison of methods for the detection of the r′<sup>s</sup> haplotype

Moulds, Joann M.; Noumsi, Ghislain; Billingsley, Katrina

doi:10.1111/trf.12956

Cited by 7 publications

(9 citation statements)

References 22 publications

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“…This was most relevant for prediction of Rh antigens, more specifically for individuals with the r's phenotype and other partial c and e antigens harbouring the same 733G mutation. A prior study documents the advantage of ID CORE XT when determining partial Rh antigens as compared to the HEA BeadChip . There were no reports of typing discrepancies that were resolved in favour of the reference method.…”

Section: Discussionmentioning

confidence: 82%

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Evaluation of a red‐blood‐cell genotyping platform, ID CORE XT, within a hospital transfusion service

Hayes

Seo

Abumuhor

et al. 2019

ISBT Science Series

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Background and Objectives Red-blood-cell genotyping is used to provide extended matched blood, resolve typing discrepancies and predict phenotype in recently transfused patients or patients with a positive direct antiglobulin test. The aim of this study was to complete a performance and operational evaluation of a genotyping system, ID CORE XT (Progenika Biopharma-Grifols, Barcelona, Spain), within a hospital transfusion service, using the current genotyping method as a reference for comparison.Materials and Methods DNA was extracted from blood donor and patient samples and tested using ID CORE XT and the reference method. Predicted RBC phenotype, turnaround time and hands-on time were compared. A questionnaire was administered to each clinical laboratory scientist to evaluate each platform's ease of use.Results A total of 438 samples were tested and overall agreement between methods was 99Á99%, with a single M antigen typing discrepancy noted. DNA sequencing revealed that ID CORE XT incorrectly designated the antigen status as M-negative, resulting in a false-negative M antigen predicted phenotype result. ID CORE XT showed a 24Á5% and 51Á3% reduction in mean turnaround time and hands-on time, respectively, as compared to the reference method.Conclusion ID CORE XT and the reference method are highly concordant regarding predicted phenotype. ID CORE XT offers several operational advantages, making it suitable for a busy hospital transfusion service.

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Section: Discussionmentioning

confidence: 82%

“…A prior study documents the advantage of ID CORE XT when determining partial Rh antigens as compared to the HEA BeadChip. [15] There were no reports of typing discrepancies that were resolved in favour of the reference method.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a red‐blood‐cell genotyping platform, ID CORE XT, within a hospital transfusion service

Hayes

Seo

Abumuhor

et al. 2019

ISBT Science Series

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“…Within 4 years, Milwaukee has received requests for reference testing by RBC genotyping in 2257 patient samples (Table ) . Among the 379 samples tested for partial D, 155 (41%) were confirmed as partial D. Among those, 75 (48%) patients carried at least one DAU allele, of which eight were shown to carry a DAU‐4 or DAU‐5 allele hemizygously.…”

Section: Resultsmentioning

confidence: 99%

“… Five DAU‐0/RHD*01 , one DAU‐0/RHD* Ψ, one DAU‐0/DIIIa‐CE(4‐7)‐D , one DAU‐1/RHD* Ψ, one DAU‐3/RHD*01 , one DAU‐3/weak D Type 4.2 , and one DAU‐3/DIIIa‐CE(4‐7)‐D …”

Section: Resultsmentioning

confidence: 99%

The DAU cluster: a comparative analysis of 18 RHD alleles, some forming partial D antigens

et al. 2016

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Background The Rh system is the most complex and polymorphic blood group system in humans with more than 460 alleles known for the RHD gene. The DAU cluster of RHD alleles is characterized by the single nucleotide change producing the p.Thr379Met amino acid substitution. It is called the DAU-0 allele and has been postulated to be the primordial allele, from which all other alleles of the DAU cluster have eventually evolved. Study design and methods For 2 novel DAU alleles, the nucleotide sequences of all 10 exons as well as adjacent intronic regions, including the 5’ and 3’ untranslated regions (UTR), were determined for the RHD and RHCE genes. A phylogenetic tree for all DAU alleles was established using the neighbor-joining method with Pan troglodytes as root. Standard hemagglutination and flow cytometry tests were performed. Results We characterized 2 DAU alleles, DAU-11 and DAU-5.1, closely related to DAU-3 and DAU-5 respectively. A phylogenetic analysis of the 18 known DAU alleles indicated point mutations and interallelic recombination contributing to diversification of the DAU cluster. Conclusions The DAU alleles encode a group of RhD protein variants, some forming partial D antigens known to permit anti-D in carriers; all are expected to cause anti-D alloimmunization in recipients of red cell transfusions. The DAU alleles evolved through genomic point mutations and recombination. These results suggest that the cluster of DAU alleles represent a clade, which is concordant with our previous postulate that they derived from the primordial DAU-0 allele.

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“…Certain red cell antigens cannot be well typed due to limited availability of anti‐sera such as those against antigens from the Vel, Dombrock and Colton blood groups, for which molecular typing may be a better choice. Molecular typing can also facilitate resolution of discrepancies in blood typing as well as resolution of weak reactions, such as in D variants and U VAR . Recognition of rare variants by molecular typing especially among transfusion dependant patients such as in sickle cell disease has been consistently shown to improve patient management and outcome in terms of providing extended antigen‐matched blood .…”

Section: Applications For Molecular Blood Group Typing In Patient Manmentioning

confidence: 99%

Basic aspects, application and platforms currently available for molecular blood grouping of donor and patient population

Nadarajan

2018

ISBT Science Series

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The majority of allelic variants conferring red cell blood group antigens are attributable to single nucleotide polymorphisms, which are immensely amenable to routine genotyping methods such as PCR‐RFLP, SSP, SSO and fluorescent‐based real‐time PCR assays. These techniques are feasible in a low‐ to medium‐throughput setting. Multiplexing and use of solid surfaces or beads for probe capture have further improved the throughput and turnaround time allowing multiple alleles to be interrogated simultaneously. Novel approaches, as in the use of MALDI‐TOF mass spectrometry, appear as an attractive option for accurate, cost‐effective, high‐throughput SNP genotyping within major blood centres. Massively parallel targeted sequencing is likely the next step in the evolution of RBC genotyping. In the hospital setting, genotyping is unlikely to supplant serological typing but would rather be complementary to it. Scenarios where it would play an important role would be in the transfusion management of transfusion dependant patients such as in sickle cell anaemia or in situations where RBC phenotyping cannot be reliably undertaken as in auto‐immune haemolytic anaemia and haemolytic disease of foetus and newborn. Genotyping would also be useful where anti‐sera are not readily available or are of weak potency. Molecular typing is likely to, however, show its greatest potential in donor genotyping to establish pools of fully typed donors as well as identify donors with rare blood groups. Such an exercise will facilitate prompt and efficient delivery of best‐matched blood for patients.

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A comparison of methods for the detection of the r′^s haplotype

Abstract: The predominant type of r'(s) in African-Americans is Type 1, which can be detected either by a reagent anti-C containing Clone MS24 or by IDCORE XT. However, serology cannot differentiate between a normal C allele and the hybrid.

Cited by 7 publications

References 22 publications

Evaluation of a red‐blood‐cell genotyping platform, ID CORE XT, within a hospital transfusion service

Evaluation of a red‐blood‐cell genotyping platform, ID CORE XT, within a hospital transfusion service

The DAU cluster: a comparative analysis of 18 RHD alleles, some forming partial D antigens

Basic aspects, application and platforms currently available for molecular blood grouping of donor and patient population

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A comparison of methods for the detection of the r′s haplotype

Abstract: The predominant type of r'(s) in African-Americans is Type 1, which can be detected either by a reagent anti-C containing Clone MS24 or by IDCORE XT. However, serology cannot differentiate between a normal C allele and the hybrid.

Cited by 7 publications

References 22 publications

Evaluation of a red‐blood‐cell genotyping platform, ID CORE XT, within a hospital transfusion service

Evaluation of a red‐blood‐cell genotyping platform, ID CORE XT, within a hospital transfusion service

The DAU cluster: a comparative analysis of 18 RHD alleles, some forming partial D antigens

Basic aspects, application and platforms currently available for molecular blood grouping of donor and patient population

Contact Info

Product

Resources

About

A comparison of methods for the detection of the r′^s haplotype