A comparison of modifications of the McMaster method for the enumeration of Ascaris suum eggs in pig faecal samples

Pereckienė, Asta; Kaziūnaitė, V.; Vyšniauskas, Antanas; Petkevičius, Saulius; Malakauskas, Alvydas; Šarkūnas, Mindaugas; Taylor, Mike

doi:10.1016/j.vetpar.2007.04.014

Cited by 55 publications

(41 citation statements)

References 12 publications

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“…The results of our study are in accordance with the opinion expressed by Mes (Mes et al 2001;Mes 2003) and Pereckiene et al (2007). The concentrated McMaster method (Roepstorff and Nansen 1998), which examines 4 g of faeces and uses the lowest multiplication factor (20) from all tested methods (33 and 67), was the best technique for estimating EPG in all tested levels.…”

Section: Discussionsupporting

confidence: 89%

“…From the work of certain authors (Cringoli et al 2004;Dunn and Keymer 1986;Pereckiene et al 2007), it is evident that methods which use a low dilution factor (optimum 1:10-1:15) produce better results than methods with a ratio of 1:30 or more.…”

Section: Discussionmentioning

confidence: 99%

“…Its use has been described in detail in many diagnostics, both animal (Coles et al 1992;Karamon et al 2008;Morgan et al 2005;Nichols and Obendorf 1994;Pereckiene et al 2007;Rinaldi et al 2007;Ward et al 1997) and human (Bondarenko et al 2009;Flohr et al 2007;Stephenson et al 1989). Different McMaster method modifications use various weight of faeces examined, volumes and types of flotation solutions, sample dilutions, flotation times, applications of additional centrifugation, durations and speeds of centrifugation, numbers of sections of the McMaster slide counted and different coefficients for interpretation (Cringoli et al 2004;Karamon et al 2008;Pereckiene et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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Which McMaster egg counting technique is the most reliable?

et al. 2011

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This study focuses on the comparison of three selected modifications of the McMaster counting technique, namely the McMaster method modified by Wetzel (W) and Zajíček (Z), as well as the concentration McMaster technique according to Roepstorff and Nansen (R&N). These modifications differ in the weights of faeces examined (W, 2 g/Z, 1 g/R&N, 4 g), flotation solutions (W, NaCl/Z, MgSO(4) + Na(2)S(2)O(3)/R&N, NaCl + glucose), centrifugation (W, none/Z, 2,000 RPM for 2 min and 2,000 RPM for 1 min/R&N, 1,200 RPM for 5 min), number of McMaster chambers investigated (W, 3/Z, 2/R&N, 2), and multiplication factors used (W, 67/Z, 33/R&N, 20). To investigate the sensitivity and reliability of these methods, nematode eggs (Teladorsagia circumcincta) were used. Parasite elements are distributed through negative binomial distribution in naturally infected host faeces, and the number of parasite elements in a given amount of faeces sample is unknown to man. Therefore, we decided to prepare the exact number of eggs which were added to the parasite negative faeces; the faecal sample was then investigated. From this perspective, this is the first time a comparison of the McMaster methods has been so accurately investigated. This approach allows us to evaluate the real sensitivity and reliability of the tested method. As the findings of this study indicate, the highest sensitivity and reliability were obtained using the Roepstorff and Nansen modification. This McMaster modification is able to detect 20 eggs per sample (in 70% of samples). Concentrations of 200 and 500 eggs can be found in almost 100% of samples. Moreover, this method is simple, cheap and fast. For these reasons, we can recommend this method for routine veterinary practice.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Which McMaster egg counting technique is the most reliable?

et al. 2011

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“…The high prevalence of ascarid infections in puppies is in accordance with the transmission pattern of the parasite which is mainly by transplacental and transmammary routes in the first few days of puppy's life which increase the occurrence of the parasite at an early age. The acquired age-dependent immunity by adult dogs decreases the establishment as well as the fecundity of the parasite [36], probably as consequence of repeated exposure.…”

Section: Discussionmentioning

confidence: 99%

Gastrointestinal Helminth Parasites of Domestic Dogs in Ilesa, Osun State, Nigeria: A Faecal Examination Survey Study

Sowemimo¹,

Ayanniyi²

2017

J Bacteriol Parasitol

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Toxocariasis remain the most important parasitic infections affecting companion animals worldwide and pose a risk to animal and human health. There is still inadequate information on these infections in dogs in some parts of Nigeria. A cross sectional study was undertaken between August and December, 2015 to determine the prevalence and intensity of intestinal helmiths of domestic dogs in Ilesa, Osun State, Nigeria. A total of 174 faecal samples collected randomly from dogs were processed and examined for helminth eggs using modified Kato-Katz technique. The overall prevalence of gastrointestinal helminth was 41.7%. T. canis was the most frequently observed helminth parasites with a prevalence of 30.5%, while prevalence of 28.2% and 9.8% were obtained for A. caninum and D. caninum respectively. Prevalence patterns in T. canis were age dependent showing a decreasing prevalence with age of host. There was a significantly (p<0.05) greater prevalence and intensity in dogs that roam freely (39.8%; 118.2 ± 25.7 epg) as compared with kenneled dogs (10.7%; 19.3 ± 11.2 epg). T. canis, A. caninum and D. caninum were the zoonotic gastrointestinal helminths prevalent in dogs in the study area. Interventions should include establishment of a program focusing on zoonotic diseases.

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“…The modified Mcmaster counting technique as described by Soulsby (1986) and Tibor (1999) was performed to determine the parasitic load (egg per gram/oocysts per gram). Salt sugar solution at specific gravity of 1.27, was used as flotation fluid for performing the floatation technique as well as for the McMaster technique (Pereckiene et al 2007). Floatation technique is usually performed to identify eggs of Cestodes and Nematodes.…”

Section: Methodsmentioning

confidence: 99%

Gastrointestinal parasitic infection in diverse species of domestic birds of Manipur, India

Singh

Mohilal

2016

J Parasit Dis

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Domesticated birds (515) consisting of 185 broiler (Gallus gallus domesticus), 130 layers (Gallus Sp), 75 free range local chicken (Gallus domesticus), 40 house pigeon (Columba livia), 70 ducks (Anas platyrhynchos) and 15 turkeys (Melleagris gallopavo) reared in valley districts of Manipur were investigated for intestinal protozoan and helminthic infections. Fresh faecal samples of these birds were examined microscopically by direct wet smear with 2.5 % potassium dichromate (K 2 Cr 2 O 7 ). Of these 412 (80 %) were found to be infected with different species of gastrointestinal parasites. The highest (90.8 %) prevalence of these parasitic infections was found in broiler followed by layer (78.5 %), duck (75.7 %), house pigeon (75 %), Turkey (66.7 %) and free range local chicken (65.3 %), etc. A total of 10 species of intestinal parasites were encountered. Among these, the protozoans Eimeria and Isospora were the most common (68.3 %) followed by mixed infection of protozoan and Helminths.

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A comparison of modifications of the McMaster method for the enumeration of Ascaris suum eggs in pig faecal samples

Cited by 55 publications

References 12 publications

Which McMaster egg counting technique is the most reliable?

Which McMaster egg counting technique is the most reliable?

Gastrointestinal Helminth Parasites of Domestic Dogs in Ilesa, Osun State, Nigeria: A Faecal Examination Survey Study

Gastrointestinal parasitic infection in diverse species of domestic birds of Manipur, India

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