A comparison of mRNA and circRNA expression between squamous cell carcinoma and adenocarcinoma of the lungs

Yu, Min; Tian, Yingxuan; Wu, Min; Gao, Jinglong; Wang, Yuan; Liu, Fuqiang; Sheng, Sen; Huo, Shufen; Bai, Jun

doi:10.1590/1678-4685-gmb-2020-0054

Cited by 10 publications

(7 citation statements)

References 32 publications

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“…In addition, ROC curve showed that CDK6 and LYPLA1 had good diagnostic e cacy for NSCLC patients. A consistent observation in a recent study revealed that high level of CDK6 indicated a poor prognosis for LUAD patients[49]. Altogether, CDK6 and LYPLA1 in serum can be used as diagnostic and prognostic markers of NSCLCs.…”

supporting

confidence: 76%

Expression and Diagnostic Efficacy of miR-126-5p and miR-34c-3p in Serum Extracellular Vesicles of Non-Small Cell Lung Cancer Patients

Zhao¹,

Hang²,

Wang³

et al. 2021

Preprint

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Background: Non-small cell lung cancer (NSCLC) is a disease with quite grave prognosis. This study explored the diagnostic efficiency of miR-126-5p and miR-34c-3p in serum extracellular vesicles (EVs) in NSCLC patients.Methods: Serum EVs were extracted from NSCLC patients and healthy people and verified. The expression of miR-126-5p and miR-34c-3p in serum EVs were tested. Correlation of miR-126-5p and miR-34c-3p expression and diagnosis, prognosis and pathological characteristics (age, gender, tumor size, clinical stage, and lymph node metastasis) of NSCLC patients was analyzed. The downstream targets of miR-126-5p and miR-34c-3p were predicted and their roles in diagnosis and prognosis of NSCLC patients were evaluated.Results: miR-126-5p and miR-34c-3p were poorly expressed in serum EVs of NSCLC patients and their low expressions were associated with clinical stage, lymph node metastasis and prognosis of NSCLC patients and could be used as biomarkers for diagnosis. As the common target genes of miR-126-5p and miR-34c-3p, LYPLA1 and CDK6 were highly expressed in serum EVs and were associated with poor prognosis in NSCLC patients.Conclusion: Lowly expressed miR-126-5p and miR-34c-3p in serum EVs of NSCLC patients can serve as biomarkers for diagnosis and are linked with prognosis. As common targets of miR-126-5p and miR-34c-3p, LYPLA1 and CDK6 are also associated with poor prognosis in NSCLC patients.

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supporting

confidence: 76%

Expression and Diagnostic Efficacy of miR-126-5p and miR-34c-3p in Serum Extracellular Vesicles of Non-Small Cell Lung Cancer Patients

Zhao¹,

Hang²,

Wang³

et al. 2021

Preprint

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“…The ceRNA network involving lncRNAs, circRNAs, and miRNAs in the regulation of cancer development has been validated by many studies, in which lncRNAs/circRNAs may act as ceRNAs by competitively binding to miRNAs to regulate the expression levels of miRNA target mRNAs [ 24 ]. Importantly, the function of circRNAs in cancer cells has been illustrated, including in LUSC [ 25 , 26 ]. In the present research, it was observed through FISH assay that circCNN2 was mainly located in the cytoplasm of LUSC cells, which suggested the possibility of the ceRNA pattern.…”

Section: Discussionmentioning

confidence: 99%

circCNN2 Accelerates Cell Proliferation and Invasion in Lung Squamous Cell Carcinoma via Regulating miR-184/E2F1 and Activating MAPK Signaling Pathway

et al. 2022

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Circular RNAs (circRNAs) have been demonstrated as potential biomarkers for the diagnosis and treatment of human diseases. Previous studies have unveiled the carcinogenic role of circRNA_102399 (circCNN2) in lung cancer. Through the UALCAN database, it was found that CNN2, the parent gene of circCNN2, was specifically highly expressed in human lung squamous cell carcinoma (LUSC) cells, but the regulatory mechanism of circCNN2 in LUSC is unclear. In this study, circCNN2 expression in LUSC cells was examined via RT-qPCR, and its effects on LUSC progression was verified through functional assays. The subcellular localization of circCNN2 was identified via FISH assay, and the underlying mechanism of circCNN2 on the activation of MAPK signaling pathway was determined through mechanism experiments. Results demonstrated that circCNN2 was upregulated in LUSC cells, and it promoted LUSC cell proliferation and invasion. Besides, circCNN2 acted as a competing endogenous RNA (ceRNA) to sponge miR-184 to upregulate E2F transcription factor 1 (E2F1) expression. Furthermore, it was verified that circCNN2 activated the generic mitogen-activated protein kinases (MAPK) signaling pathway through E2F1 and thus promoting LUSC progression. In a word, this study indicated that circCNN2 activated MAPK signaling pathway through the miR-184/E2F1 axis to promote proliferation and invasion of LUSC cells.

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“…Most of these genes are associated with tumor cell proliferation, differentiation and invasion [26][27][28][29][30].…”

Section: Discussionmentioning

confidence: 99%

Deciphering biomarker related to 12 programmed cell death to predict prognosis and response to immunotherapy in lung squamous cell carcinoma

Jiang,

Dao,

Wang

et al. 2023

Preprint

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Background Different programmed cell death (PCD) plays different roles in lung squamous cell carcinoma (LUSC). We integrated twelve programmed cell death patterns, investigated the expression patterns of PCD-related genes to identify promising PCD-related biomarkers. Methods Twelve PCD patterns (apoptosis, pyroptosis, necroptosis, cuproptosis, entotic cell death, autophagy-dependent cell death, netotic cell death, parthanatos, ferroptosis, lysosome-dependent cell death, oxeiptosis and alkaliptosis) were analyzed for model construction, resulting in 1388 PCD-related genes. We explored the expression changes of PCD-related genes in LUSC patients from TCGA database, and constructed a combined prognostic signature by Cox regression analysis and LASSO Cox regression analysis. The independent prognostic performance of the gene signature was evaluate based on consensus clustering, univariate and multivariate Cox regression and Kaplan–Meier survival. The GEO dataset was used for validation. Finally, we investigated the role of the immune microenvironment in different prognosis groups. Results We constructed a network of seven PCD-related genes (FGA, CHEK2, PTGIS, CSF2, STXBP1, NACC2, TFR2). Utilized these 7-gene network to establish a cell death index (CDI) and grouped patients using the median of CDI. We found that LUSC patients with low CDI had a better prognosis. More importantly, CDI was associated with tumor microenvironment components according to integrated analysis, and the response to immunotherapy in the low CDI group was better than that in the high CDI group. Conclusion Our study identified 7-gene network based on PCD to establish a new model of CDI to predict the clinical prognosis of LUSC patients. We proposed that CDI may serve as a new biomarker to predict the prognosis and immunotherapy efficacy in LUSC.

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A comparison of mRNA and circRNA expression between squamous cell carcinoma and adenocarcinoma of the lungs

Cited by 10 publications

References 32 publications

Expression and Diagnostic Efficacy of miR-126-5p and miR-34c-3p in Serum Extracellular Vesicles of Non-Small Cell Lung Cancer Patients

Expression and Diagnostic Efficacy of miR-126-5p and miR-34c-3p in Serum Extracellular Vesicles of Non-Small Cell Lung Cancer Patients

circCNN2 Accelerates Cell Proliferation and Invasion in Lung Squamous Cell Carcinoma via Regulating miR-184/E2F1 and Activating MAPK Signaling Pathway

Deciphering biomarker related to 12 programmed cell death to predict prognosis and response to immunotherapy in lung squamous cell carcinoma

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