A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays

Tam, Justina; Puig, Helena de; Yen, Chun-Wan; Bosch, Irene; Gómez-Márquez, José; Clavet, Charles; Hamad‐Schifferli, Kimberly; Gehrke, Lee

doi:10.1080/15321819.2016.1269338

Cited by 49 publications

(47 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To estimate the NS1 concentration using the Raman scans, signal intensity based on a Langmuir curve was estimated because GNP intensities have been observed to exhibit Langmuir-like concentration dependences 38,39 . Experiments were run at 5 different NS1 concentrations (Figure S5).…”

Section: Resultsmentioning

confidence: 99%

Design of SERS nanotags for multiplexed lateral flow immunoassays

Sánchez-Purrà

Roig-Solvas

Versiani

et al. 2017

Mol. Syst. Des. Eng.

Self Cite

View full text Add to dashboard Cite

Surface enhanced Raman spectroscopy (SERS) has been attractive for enhancing the sensitivity of lateral flow immunoassays (LFA). A format that has enabled specific detection of biomarkers is to use Raman reporter molecules linked to gold nanoparticles (NPs), which are conjugated to antibodies specific for the target of interest. Many factors such as the NP and Ab properties and the method of signal readout impact the sensitivity of a SERS based immunoassay. To understand how to optimize assay sensitivity, we studied SERS readouts of multiplexed sandwich immunoassays for the zika and dengue non-structural protein 1 (NS1) biomarkers as a test case. We investigated the effect of NP shape on the SERS enhancement of the reporter molecules 1,2-bis(4-pyridyl)ethylene (BPE) and 4-mercaptobenzoic acid (MBA). We also performed SERS imaging of test lines to map the spatial distribution of signal in test lines on the nitrocellulose. Finally, we used a modified least squares analysis to differentiate reporter contributions.

show abstract

Section: Resultsmentioning

confidence: 99%

Design of SERS nanotags for multiplexed lateral flow immunoassays

Sánchez-Purrà

Roig-Solvas

Versiani

et al. 2017

Mol. Syst. Des. Eng.

Self Cite

View full text Add to dashboard Cite

show abstract

“…For example, Langmuir isotherms modified to include surface terms can be used to separate the impact of the surface modifications on antibody-antigen binding in planar and NP surfaces, and can also be applied to LFAs. [48, 52, 53]…”

Section: Towards Improved Nanotechnology-enabled Lfasmentioning

confidence: 99%

Challenges of the Nano–Bio Interface in Lateral Flow and Dipstick Immunoassays

Puig

Bosch²,

Gehrke

et al. 2017

Trends in Biotechnology

Self Cite

102

View full text Add to dashboard Cite

Lateral flow assays (LFAs) are highly attractive for point of care diagnostics for infectious disease, food safety, and many other medical uses. The unique optical, electronic and chemical properties that arise from the nanostructured and material characteristics of nanoparticles provide an opportunity to increase LFA sensitivity and impart novel capabilities. However, interfacing to nanomaterials in complex biological environments is challenging and can result in undesirable side effects such as non-specific adsorption, protein denaturation, and steric hindrance. These issues are even more acute in LFAs, where there are many different types of inorganic-biological interfaces, often of complex nature. Therefore, the unique properties of nanomaterials for LFAs must be exploited in a way that addresses these interface challenges.

show abstract

“…In the recent years, several biosensors have been developed for rapid detection of NS1, such as enzyme immunoassay, 7 long-range surface plasmon waveguides, 8 electrochemical lateral flow immunosensors, 9 and colorimetric sensors. 10,11 Among these, colorimetric sensors exhibit significant advantages such as simplicity, real time naked eye detection, and no external power requirement. This has led to the development of lateral flow immunoassays based on colorimetric detection schemes, as an alternative to the conventional laboratory based assays.…”

Section: Introductionmentioning

confidence: 99%

Tapered lateral flow immunoassay based point-of-care diagnostic device for ultrasensitive colorimetric detection of dengue NS1

et al. 2018

View full text Add to dashboard Cite

Dengue virus, a family member, has emerged as a major worldwide health concern, making its early diagnosis imperative. Lateral flow immunoassays have been widely employed for point-of-care diagnosis of dengue because of their rapid naked eye readouts, ease of use, and cost-effectiveness. However, they entail a drawback of low sensitivity, limiting their usage in clinical applications. Herein, we report a novel lateral flow immunoassay for detection of dengue leveraging on the benefits of gold decorated graphene oxide sheets as detection labels and a tapered nitrocellulose membrane. The developed assay allows for rapid (10 min) and sensitive detection of dengue NS1 with a detection limit of 4.9 ng mL, ∼11-fold improvement over the previously reported values. Additionally, the clinical application of the developed assay has been demonstrated by testing it for dengue virus spiked in human serum. The reported lateral flow immunoassay shows significant promise for early and rapid detection of several target diseases.

show abstract

A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays

Cited by 49 publications

References 39 publications

Design of SERS nanotags for multiplexed lateral flow immunoassays

Design of SERS nanotags for multiplexed lateral flow immunoassays

Challenges of the Nano–Bio Interface in Lateral Flow and Dipstick Immunoassays

Tapered lateral flow immunoassay based point-of-care diagnostic device for ultrasensitive colorimetric detection of dengue NS1

Contact Info

Product

Resources

About