A Comparison of Polyglycolic Acid Versus Type 1 Collagen Bioabsorbable Nerve Conduits in a Rat Model: An Alternative to Autografting

Waitayawinyu, Thanapong; Parisi, Debra M.; Miller, Brian A.; Luria, Shai; Morton, H. Josette; Chin, Simon H.; Trumble, Thomas E.

doi:10.1016/j.jhsa.2007.07.015

Cited by 105 publications

(73 citation statements)

References 53 publications

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“…6 We found that after DEST repair, the SFI reached a mean of −36.3 at 16 weeks, which corresponds well with the results of previous studies performed with different conduits. 12,13,18,31,36 Similarly, there was no neuroma formation, which has been reported in other tubular conduits. 18,30,31,37 MacKinnon et al measured the axon density of a healthy rat sciatic nerve to be 11°882/mm 2 and noted that the density increases distal to the repair site after experimental injury and subsequent repair.…”

Section: Discussionsupporting

confidence: 69%

Distal Inside-Out Epineural Sliding Technique to Repair Segmental Nerve Defects

Luokkala

Ryhänen

Näpänkangas

et al. 2016

Hand (New York, N,Y.)

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Background: The repair of a segmental peripheral nerve injury is a clinical challenge. Several studies have been performed to determine superior methods for overcoming nerve gaps. The purpose of this study was to investigate if the inside-out slided epineurium of the distal segment of an injured nerve can serve as a conduit to bridge a short nerve defect (10 mm). Methods: Nineteen sciatic nerves in Sprague-Dawley rats were transected, and a 10-mm gap was left between the ends. A section of distal epineurium was pulled inside out to bridge the gap. Walking track analysis was performed, and the sciatic function index (SFI) was calculated. Wet muscle mass and withdrawal reflex were measured. The density of axon fibers at different levels of repaired nerves was determined, and histological analysis was performed at 16 weeks. Results: The mean SFI improved from −81.0 at 4 weeks to 36.3 at 16 weeks. The axon densities showed regeneration through the epineural tube, and 5 of the rats demonstrated a withdrawal reflex. The weight of the tibialis anterior muscle of the injured limb at 16 weeks was 59% that of the uninjured side. Conclusions: The distal epineural sheath tube provided a size-matched conduit between the nerve stumps, with no histological donor-site morbidity. Histologically, regeneration occurred through the epineural tube without neuroma formation, and functional recovery was comparable to that of previous studies of nerve repair techniques. Technique may be an addition to the armamentarium of tools used to treat segmental nerve defects.

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Section: Discussionsupporting

confidence: 69%

Distal Inside-Out Epineural Sliding Technique to Repair Segmental Nerve Defects

Luokkala

Ryhänen

Näpänkangas

et al. 2016

Hand (New York, N,Y.)

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“…Moreover, some synthetic conduits have already demonstrated the similarity to autograft in these small animals. 10,12,31 A larger animal model where longer nerve gaps can be evaluated is clearly needed. In the present study, we described a new experimental model for motor nerve regeneration where nerve defects can reach up to 8 cm.…”

Section: Discussionmentioning

confidence: 99%

Description and validation of isometric tetanic muscle force test in rabbits

et al. 2011

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Isometric tetanic muscle force has been described in a rat model to evaluate motor recovery in a segmental sciatic nerve defect reconstructions. However, to test longer nerve defects, an alternative and larger animal model is necessary. The purpose of this study is to describe and validate a technique for isometric force measurement of the tibialis anterior (TA) muscle in New Zealand rabbits. Muscle preload and electrical stimulation parameters were optimized to obtain the highest tetanic contraction bilaterally in 10 animals. Electrophysiology, muscle weight, peroneal nerve length, and histomorphometry were also analyzed. Only the peroneal nerve length and the ratio of highest muscle force/muscle weight demonstrated the equivalence between the sides. A small variability of TA muscle force and TA muscle weight was observed between the sides suggesting dominance. Optimization of electrical stimulation and preload as well as the use of correct anesthesia were fundamental to acquire the highest muscle force.

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“…PGGCs can not only offer effective aids for regenerating nerves but also accelerate favourable nerve-functional recovery when compared with nonporous genipin cross-linked gelatine conduits [42] chitosan-polylactic acid (PLA) 10 12 weeks, distal 6275 + 2000 axonal quantity of chitosan-PLA tubes are higher than silicone rubber tubes (2648 + 685) [43] proanthocyanidin (PA) crosslinked gelatine 10 eight weeks, midpoint mostly unmyelinated axons the peak amplitude, area under the MAP curve, and the histological observations of regenerated nerves all increase with the recovery period [7] polyglycolic acid (PGA) [36]. After completion of their guiding function, the GCC conduits degraded and were well integrated with the regenerating nerve tissues.…”

Section: Discussionmentioning

confidence: 99%

“…) than those in the conduits made of various biodegradable materials reported in the literature, such as the chitosan [17], the PLA [2], the PGA [7], the proanthocyanidin (PA) crosslinked gelatine [43] and the genipin cross-linked gelatine (GGT) [22,30]. In addition, the temporal and spatial progresses of cellular activity within the GCC conduit are better than those seen for experiments using silicone rubber nerve guides [41,42], which have largely been used in clinical practice.…”

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confidence: 99%

Novel use of biodegradable casein conduits for guided peripheral nerve regeneration

Hsiang

Tsai

et al. 2011

J. R. Soc. Interface.

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Recent advances in nerve repair technology have focused on finding more biocompatible, nontoxic materials to imitate natural peripheral nerve components. In this study, casein protein cross-linked with naturally occurring genipin (genipin-cross-linked casein (GCC)) was used for the first time to make a biodegradable conduit for peripheral nerve repair. The GCC conduit was dark blue in appearance with a concentric and round lumen. Water uptake, contact angle and mechanical tests indicated that the conduit had a high stability in water and did not collapse and cramped with a sufficiently high level of mechanical properties. Cytotoxic testing and terminal deoxynucleotidyl transferase dUTP nick-end labelling assay showed that the GCC was non-toxic and non-apoptotic, which could maintain the survival and outgrowth of Schwann cells. Non-invasive real-time nuclear factor-kB bioluminescence imaging accompanied by histochemical assessment showed that the GCC was highly biocompatible after subcutaneous implantation in transgenic mice. Effectiveness of the GCC conduit as a guidance channel was examined as it was used to repair a 10 mm gap in the rat sciatic nerve. Electrophysiology, labelling of calcitonin gene-related peptide in the lumbar spinal cord, and histology analysis all showed a rapid morphological and functional recovery for the disrupted nerves. Therefore, we conclude that the GCC can offer great nerve regeneration characteristics and can be a promising material for the successful repair of peripheral nerve defects.

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A Comparison of Polyglycolic Acid Versus Type 1 Collagen Bioabsorbable Nerve Conduits in a Rat Model: An Alternative to Autografting

Cited by 105 publications

References 53 publications

Distal Inside-Out Epineural Sliding Technique to Repair Segmental Nerve Defects

Distal Inside-Out Epineural Sliding Technique to Repair Segmental Nerve Defects

Description and validation of isometric tetanic muscle force test in rabbits

Novel use of biodegradable casein conduits for guided peripheral nerve regeneration

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