A comparison of reproductive outcome using different sperm selection techniques; density gradient, testicular sperm, PICSI, and MACS for ICSI patients with abnormal DNA fragmentation index

Hozaien, M.M.; Elqusi, Khaled; Hassanen, Eman Mohamed; Hussin, Abdul Aziz; Alkhader, Hanaa Ahmed; Tanbouly, S.M. El; El-qassaby, S.G.; Zaki, Hosam

doi:10.1016/j.fertnstert.2018.07.071

Cited by 3 publications

(4 citation statements)

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“…The use of nanoparticles to target physical and physiological characteristics of sperm (motility, directionaliy, apoptosis, intact acrosome, etc.) can help predict whether a semen sample is suitable for assisted reproductive techniques (ART), leading to successful fertilization [15, 16]. Routine techniques for sperm purification such as swim-up [17], discontinuous percoll [18], albumin filtration [19], density gradient centrifugations (DGC) [8], and magnetic-assisted cell sorting (MACS) [20, 21] yield low numbers of motile spermatozoa, but appear more suitable for small-scale applications such as in vitro fertilization (IVF) and intra-cytoplasmic sperm injection (ICSI).…”

Section: Introductionmentioning

confidence: 99%

“…Routine techniques for sperm purification such as swim-up [17], discontinuous percoll [18], albumin filtration [19], density gradient centrifugations (DGC) [8], and magnetic-assisted cell sorting (MACS) [20, 21] yield low numbers of motile spermatozoa, but appear more suitable for small-scale applications such as in vitro fertilization (IVF) and intra-cytoplasmic sperm injection (ICSI). Comparative studies have revealed the preponderant effects of MACS for the selection of viable spermatozoa [16, 20], leading to better reproductive outcomes than other techniques (i.e., DGC). Despite the numerous advantages of MACS (e.g., simple, rapid, affordable), its performance is limited to less than 10 9 spermatozoa processed for a single target of sperm viability parameter (i.e., apoptosis) [16, 22], which drastically limits its applicability in the swine industry.…”

Section: Introductionmentioning

confidence: 99%

“…Comparative studies have revealed the preponderant effects of MACS for the selection of viable spermatozoa [16, 20], leading to better reproductive outcomes than other techniques (i.e., DGC). Despite the numerous advantages of MACS (e.g., simple, rapid, affordable), its performance is limited to less than 10 9 spermatozoa processed for a single target of sperm viability parameter (i.e., apoptosis) [16, 22], which drastically limits its applicability in the swine industry.…”

Section: Introductionmentioning

confidence: 99%

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Nanotechnology-based approach for safer enrichment of semen with best spermatozoa

Durfey

Swistek

Liao

et al. 2019

J Animal Sci Biotechnol

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BackgroundAdvances in nanotechnology have permitted molecular-based targeting of cells through safe and biocompatible magnetic nanoparticles (MNP). Their use to detect and remove damaged spermatozoa from semen doses could be of great interest. Here, MNP were synthesized and tested for their ability to target apoptotic (annexin V) and acrosome-reacted (lectin) boar spermatozoa, for high-throughout retrieval in a magnetic field (nanoselection). The potential impacts of nanoselection on sperm functions and performance of offspring sired by sperm subjected to nanoselection were determined. Fresh harvested and extended boar semen was mixed with various amounts (0, 87.5, and 175 μg) of MNP-conjugates (Annexin V-MNP or Lectin-MNP) and incubated (10 to 15 min) for 37 °C in Exp. 1. In Exp. 2, extended semen was mixed with optimal concentrations of MNP-conjugates and incubated (0, 30, 90, or 120 min). In Exp. 3, the synergistic effects of both MNP-conjugates (87.5 μg – 30 min) on spermatozoa was evaluated, followed by sperm fertility assessments through pregnancy of inseminated gilts and performance of neonatal offspring. Sperm motion, viability, and morphology characteristics were evaluated in all experiments.ResultsTransmission electron microscopy, atomic force microscopy, and hyperspectral imaging techniques were used to confirm attachment of MNP-conjugates to damaged spermatozoa. The motility of nanoselected spermatozoa was improved (P < 0.05). The viability of boar sperm, as assessed by the abundance of reactive oxygen species and the integrity of the acrosome, plasma membrane, and mitochondrial membrane was not different between nanoselected and control spermatozoa. The fertility of gilts inseminated with control or nanoselected spermatozoa, as well as growth and health of their offspring were not different between (P > 0.05).ConclusionsThe findings revealed the benefit of magnetic nanoselection for high-throughput targeting of damaged sperm, for removal and rapid and effortless enrichment of semen doses with highly motile, viable, and fertile spermatozoa. Therefore, magnetic nanoselection for removal of abnormal spermatozoa from semen is a promising tool for improving fertility of males, particularly during periods, such as heat stress during the summer months.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nanotechnology-based approach for safer enrichment of semen with best spermatozoa

Durfey

Swistek

Liao

et al. 2019

J Animal Sci Biotechnol

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show abstract

“…To obtain good male reproduction, removing unviable or degenerated sperm cells and scavenger ROS from semen samples is important. Recent nanotechnologies reflect new prospects for developing novel and noninvasive techniques for sperm manipulation [63][64][65].…”

Section: Nanoparticles (Nps)mentioning

confidence: 99%

The Current Trends in Using Nanoparticles, Liposomes, and Exosomes for Semen Cryopreservation

Saadeldin

Khalil

Alharbi

et al. 2020

Animals

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Cryopreservation is an essential tool to preserve sperm cells for zootechnical management and artificial insemination purposes. Cryopreservation is associated with sperm damage via different levels of plasma membrane injury and oxidative stress. Nanoparticles are often used to defend against free radicals and oxidative stress generated through the entire process of cryopreservation. Recently, artificial or natural nanovesicles including liposomes and exosomes, respectively, have shown regenerative capabilities to repair damaged sperm during the freeze–thaw process. Exosomes possess a potential pleiotropic effect because they contain antioxidants, lipids, and other bioactive molecules regulating and repairing spermatozoa. In this review, we highlight the current strategies of using nanoparticles and nanovesicles (liposomes and exosomes) to combat the cryoinjuries associated with semen cryopreservation.

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The application of nanomaterials in designing promising diagnostic, preservation, and therapeutic strategies in combating male infertility: A review

Mukherjee,

Valsala Gopalakrishnan,

Mukherjee

2024

Journal of Drug Delivery Science and Technology

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A comparison of reproductive outcome using different sperm selection techniques; density gradient, testicular sperm, PICSI, and MACS for ICSI patients with abnormal DNA fragmentation index

Cited by 3 publications

References 0 publications

Nanotechnology-based approach for safer enrichment of semen with best spermatozoa

Nanotechnology-based approach for safer enrichment of semen with best spermatozoa

The Current Trends in Using Nanoparticles, Liposomes, and Exosomes for Semen Cryopreservation

The application of nanomaterials in designing promising diagnostic, preservation, and therapeutic strategies in combating male infertility: A review

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