A comparison of semen diluents on the in vitro and in vivo fertility of liquid bull semen

Abstract

Semen 3Murphy et al. 4This study examined the effect of a wide range of semen diluents on both the in vitro and in 5 vivo fertility of liquid bull semen stored for up to 3 days post collection. The semen diluents 6 BioXcell, INRA96 and Caprogen maintained sperm motility best when stored at a constant 7 temperature, however, when used in the field, at an unregulated temperature, BioXcell 8 performed poorly. This study clearly illustrates the importance of in vivo fertility data in 9 assessing semen diluents. The aim of this study was to assess the effect of semen diluent on calving rate (CR) following 10 SEMEN DILUENTS FOR LIQUID BULL SEMEN 11 A Comparison of Semen Diluents on the

Low Density Lipoprotein - important player in increasing cryoprotective efficiency of soybean lecithin-based bull semen extenders

“…Moreover, this predominantly field study revealed that the calving rate was highest when EY extender was used for semen preservation. This supports doubts concerning SL and EY extenders effectiveness (Gil et al, 2000;van Wagtendonk-de Leeuw et al, 2000;Thun et al, 2002;Aires et al, 2003;Crespilho et al, 2012;Murphy et al, 2017). The fact that artificial liposomebased extender did not exhibit such protection during semen chilling showed that other methods to improve the cryoprotective properties of bull semen extenders could be found.…”

“…Our results differ according to the extender used, which could indicate their distinct composition. Moreover, our previous study and study of Murphy et al (2017) revealed the distinct effectiveness of LDL across different soybean extenders and protection by themselves alone, respectively. Unfortunately, information about either the precise content of soybean lecithin or other components in extenders is unavailable in commercial extenders.…”

“…All previous studies by other authors dealt only with the possibility of egg yolk substitution by LDL. Attempts to examine the effect of LDL addition to animal protein-free SL extenders when there is commercially available liposome based Optixcell ® have been strongly supported by a very current study done by (Murphy et al, 2017). The authors compared the protective properties of Optixcell ® with different types of semen extenders.…”

“…2019 268 most used alternative to egg yolk, in current practice (Layek et al, 2016). Nevertheless, there is no clear consensus concerning its cryoprotective efficacy and suitability in comparison to egg yolk (Gil et al, 2000;van Wagtendonk-de Leeuw et al, 2000;Thun et al, 2002;Aires et al, 2003;Crespilho et al, 2012;Murphy et al, 2017). Another possible substitute for egg yolk is artificially prepared liposomes in the commercially available Optixcell ® extender.…”

“…Another possible substitute for egg yolk is artificially prepared liposomes in the commercially available Optixcell ® extender. However, insufficient protective efficacy was proven when compared to the aforementioned extenders (Murphy et al, 2017). Further option to overcome problems with egg yolk is using just its cryoprotective constituent -Low Density Lipoprotein (LDL) (Moussa et al, 2002).…”