A Comparison of Short- and Long-Term Soy Protein Isolate Intake and Its Ability to Reduce Liver Steatosis in Obese Zucker Rats Through Modifications of Genes Involved in Inflammation and Lipid Transport

Kozaczek, Melisa; Bottje, Walter; Kong, Byung-Whi; Albataineh, Diyana; Hakkak, Reza

doi:10.1089/jmf.2020.0180

Cited by 1 publication

(2 citation statements)

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“…Serum levels of these inflammatory mediators are usually increased in obese patients with NAFLD and the obese Zucker rat model, as reported in the studies by Hakkak et al (29) and Kozaczek et al (30). We also reported how feeding SPI reduces the expression of inflammation-related genes, such as IL-33 and IL-1B, that could be helping in the general reduction of liver steatosis observed with the SPI diet (31). In humans, studies have described a reduction in the expression of CYP3A family related to liver inflammation (76), and a study by Woolsey et al (77) reported a reduction of CYP3A family in humans and a murine model for NAFLD.…”

Section: Discussionsupporting

confidence: 80%

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Effects of Short- and Long-Term Soy Protein Feeding on Hepatic Cytochrome P450 Expression in Obese Nonalcoholic Fatty Liver Disease Rat Model

Kozaczek

Bottje²,

Albataineh³

et al. 2021

Front. Nutr.

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Obesity can lead to chronic health complications such as nonalcoholic fatty liver disease (NAFLD). NAFLD is characterized by lipid aggregation in the hepatocytes and inflammation of the liver tissue as a consequence that can contribute to the development of cirrhosis and hepatocellular carcinoma (HCC). Previously, we reported that feeding obese Zucker rats with soy protein isolate (SPI) can reduce liver steatosis when compared with a casein (CAS) diet as a control. However, the effects of SPI on cytochrome P450 (CYP) in an obese rat model are less known. In addition, there is a lack of information concerning the consumption of soy protein in adolescents and its effect in reducing the early onset of NAFLD in this group. Our main goal was to understand if the SPI diet had any impact on the hepatic CYP gene expression when compared with the CAS diet. For this purpose, we used the transcriptomic data obtained in a previous study in which liver samples were collected from obese rats after short-term (eight-week) and long-term (16-week) feeding of SPI (n = 8 per group). To analyze this RNAseq data, we used Ingenuity Pathway Analysis (IPA) software. Comparing short- vs long-term feeding revealed an increase in the number of downregulated CYP genes from three at 8 weeks of SPI diet to five at 16 weeks of the same diet (P ≤ 0.05). On the other hand, upregulated CYP gene numbers showed a small increase in the long-term SPI diet compared to the short-term SPI diet, from 14 genes at 8 weeks to 17 genes at 16 weeks (P ≤ 0.05). The observed changes may have an important role in the attenuation of liver steatosis.

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Section: Discussionsupporting

confidence: 80%

“…We can extrapolate both the short-and long-term results to adolescents and adults, respectively. Recently, we reported that SPI had a protective effect against liver steatosis (29) and proposed that SPI reduces inflammation and enhances the lipid transport out of the liver in obese Zucker rats (30,31).…”

Section: Introductionmentioning

confidence: 99%