A comparison of stilbene and chalcone synthases including a new stilbene synthase gene from Vitis riparia cv. Gloire de Montpellier

Goodwin, P. H.; Hsiang, Tom; Erickson, L.

doi:10.1016/s0168-9452(99)00175-2

Cited by 19 publications

(9 citation statements)

References 20 publications

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“…The 35S:NS-Vitis3 strawberry line was generated essentially as described by Schaart et al (2002) by transformation with the pCAMBIA2301 vector in which GUS (β-glucuronidase) was replaced by the V. riparia STS gene [ NS-Vitis3 , GenBank accession no. AF128861, Goodwin et al , 2000 ; cloned by oligonucleotides E176 and E177 (see Supplementary Table S1 available at JXB online) with kanamycin as the selectable marker under the control of the CaMV 35S promoter]. Young, folded strawberry leaves were surface-sterilized and chopped into small pieces.…”

Section: Methodsmentioning

confidence: 99%

Stilbene synthase gene transfer caused alterations in the phenylpropanoid metabolism of transgenic strawberry (Fragaria×ananassa)

Hanhineva

Kokko

Siljanen

et al. 2009

Journal of Experimental Botany

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The gene encoding stilbene synthase is frequently used to modify plant secondary metabolism with the aim of producing the self-defence phytoalexin resveratrol. In this study, strawberry (Fragaria×ananassa) was transformed with the NS-Vitis3 gene encoding stilbene synthase from frost grape (Vitis riparia) under the control of the cauliflower mosaic virus 35S and the floral filament-specific fil1 promoters. Changes in leaf metabolites were investigated with UPLC-qTOF-MS (ultra performance liquid chromatography-quadrupole time of flight mass spectrometry) profiling, and increased accumulation of cinnamate, coumarate, and ferulate derivatives concomitantly with a decrease in the levels of flavonols was observed, while the anticipated resveratrol or its derivatives were not detected. The changed metabolite profile suggested that chalcone synthase was down-regulated by the genetic modification; this was verified by decreased chalcone synthase transcript levels. Changes in the levels of phenolic compounds led to increased susceptibility of the transgenic strawberry to grey mould fungus.

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Section: Methodsmentioning

confidence: 99%

Stilbene synthase gene transfer caused alterations in the phenylpropanoid metabolism of transgenic strawberry (Fragaria×ananassa)

Hanhineva

Kokko

Siljanen

et al. 2009

Journal of Experimental Botany

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“…STS is encoded by a multigene family mainly comprising the resveratrol‐forming STS genes from grapevine ( pSV21 , pSV25 , pSV696 , pSV368 , and StSy ) [25], ( Vst1 , Vst2 , and Vst3 ) [26], and the pinosylvin‐forming STS genes from pine ( PST‐1 , PST‐2 , PST‐3 , PST‐4 , and PST‐5 ) [27]. Three novel STS genes ( pdsts1 , pdsts2 , and pdsts3 ) have been isolated from the roots of Pinus densiflora [28, 29], together with a STS gene from Vitis riparia cv Gloire de Montpellier [30]. At present, there is only one STS gene described in a monocotyledonous plant, the Sb STS1 gene isolated from Sorghum [31].…”

Section: Biosynthesis Of Stilbenesmentioning

confidence: 99%

Biosynthesis, metabolism, molecular engineering, and biological functions of stilbene phytoalexins in plants

et al. 2010

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Stilbenic compounds recently have become the focus of a number of studies in medicine and plant physiology as well as have emerged as promising molecules that potentially affect human health. Stilbenes are relatively simple compounds synthesized by plants and deriving from the phenyalanine/polymalonate route, the last and key enzyme of this pathway being stilbene synthase. Here, we review the biological significance of stilbenes in plants together with their biosynthesis pathway and their metabolism both by fungi and in planta. Special attention will be paid to the role of stilbenic molecules as phytoalexins.

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“…Other STS genes have also been isolated from pine [102–104], together with a stilbene synthase gene from Vitis riparia cv Gloire de Montpellier [105]. The Sb STS1 gene isolated from Sorghum is at present the only one STS gene described in a Monocotyledonous plant [106].…”

Section: Biosynthesis Of Resveratrolmentioning

confidence: 99%

Metabolic Engineering of Yeast and Plants for the Production of the Biologically Active Hydroxystilbene, Resveratrol

Jeandet

Delaunois

Aziz

et al. 2012

Journal of Biomedicine and Biotechnology

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Resveratrol, a stilbenic compound deriving from the phenyalanine/polymalonate route, being stilbene synthase the last and key enzyme of this pathway, recently has become the focus of a number of studies in medicine and plant physiology. Increased demand for this molecule for nutraceutical, cosmetic and possibly pharmaceutic uses, makes its production a necessity. In this context, the use of biotechnology through recombinant microorganisms and plants is particularly promising. Interesting results can indeed arise from the potential of genetically modified microorganisms as an alternative mechanism for producing resveratrol. Strategies used to tailoring yeast as they do not possess the genes that encode for the resveratrol pathway, will be described. On the other hand, most interest has centered in recent years, on STS gene transfer experiments from various origins to the genome of numerous plants. This work also presents a comprehensive review on plant molecular engineering with the STS gene, resulting in disease resistance against microorganisms and the enhancement of the antioxidant activities of several fruits in transgenic lines.

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A comparison of stilbene and chalcone synthases including a new stilbene synthase gene from Vitis riparia cv. Gloire de Montpellier

Cited by 19 publications

References 20 publications

Stilbene synthase gene transfer caused alterations in the phenylpropanoid metabolism of transgenic strawberry (Fragaria×ananassa)

Stilbene synthase gene transfer caused alterations in the phenylpropanoid metabolism of transgenic strawberry (Fragaria×ananassa)

Biosynthesis, metabolism, molecular engineering, and biological functions of stilbene phytoalexins in plants

Metabolic Engineering of Yeast and Plants for the Production of the Biologically Active Hydroxystilbene, Resveratrol

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