A comparison of subcutaneous adipose tissue proteomes in juvenile piglets with a contrasted adiposity underscored similarities with human obesity

Gondret, Florence; Guével, Blandine; Com, Emmanuelle; Vincent, Annie; Lebret, Bénédicte

doi:10.1016/j.jprot.2011.10.012

Cited by 17 publications

(20 citation statements)

References 46 publications

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“…Previous studies have shown that sEH is differentially expressed in the adipose tissues of fat piglets and during the development of obesity in humans (7,8). SNPs in pig EPHX1 have been partially identified in our previous study (9).…”

Section: Please Scroll Down For Articlementioning

confidence: 74%

Single-Nucleotide Polymorphisms in PigEPHX1Gene are Associated with Pork Quality Traits

Cho

Kwon

et al. 2015

Animal Biotechnology

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Epoxide hydrolase 1 (EPHX1) plays an important role in both the activation and detoxification of exogenous chemicals. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the highest level of EPHX1 expression occurred in Berkshire liver, which is an organ that plays a key role in detoxification. We examined EPHX1 SNPs to analyze effect on increased expression of EPHX1 gene in Berkshire liver by total of 192 pigs of a pure Berkshire line (males = 97; females = 95). As a result, two nonsynonymous SNPs (nsSNPs) of EPHX1 were found from c.685T>G and c.776C > T, and located in 5th and 6th exons, respectively, which constitute the A/b hydrolase 1 domain of epoxide hydrolase. The nsSNP c.685T > G was significant differences in meat color, protein content, collagen content, and pH24 hr. Especially, T and G alleles of the nsSNP c.685T > G were significantly associated with CIE a*/CIE b* and protein content/pH24 hr, respectively. The nsSNP c.776C > T was significant differences in drip loss and protein content. Among meat quality traits to associate with SNPs, the protein content was only significantly associated with sex. Therefore, it is suggested that nsSNP c.685T> G in EPHX1 gene is a potential to apply as appropriate DNA markers for improvement of porcine economic traits.

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Section: Please Scroll Down For Articlementioning

confidence: 74%

Single-Nucleotide Polymorphisms in PigEPHX1Gene are Associated with Pork Quality Traits

Cho

Kwon

et al. 2015

Animal Biotechnology

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“…43,[52][53][54][55][56][57][58][59] Furthermore, adipose tissue from obese pigs exhibits a cytokine expression profile consistent with the development of low-grade chronic inflammation seen in human obesity and porcine adipose tissue responds to LPS challenge to increase proinflammatory adipokine expression. 57,59,60 In the current study, the expression of leptin, adiponectin, IL-6, and TNFa mRNA was verified in neonatal adipose tissue from the Mangalica pig, a novel lard-type breed, for the first time. The down regulation of IL-6 mRNA during adipogenesis in the present study was expected since IL-6 is also down regulated during the differentiation of rodent preadipocytes and IL-6 is predominantly secreted from stromal vascular cells in human and rodent adipose tissue.…”

Section: Discussionmentioning

confidence: 85%

The proliferation and differentiation of primary pig preadipocytes is suppressed when cultures are incubated at 37°Celsius compared to euthermic conditions in pigs

et al. 2014

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Given similarities in metabolic parameters and cardiovascular physiology, the pig is well positioned as a biomedical model for metabolic disease and obesity in humans. Better understanding molecular mechanisms governing porcine adipocyte hyperplasia may provide insight into the regulation of adipose tissue development that is useful both when considering the pig as a commodity and when extrapolating porcine data to human disease. Primary cultures of pig stromal-vascular cells have served as a useful tool for investigating factors that regulate preadipocyte proliferation and differentiation. However, such cultures have generally been maintained at 37°C in vitro despite euthermia being 39°C in pigs. To address potential concerns about the physiological relevance of culturing primary pig preadipocytes under what would be hypothermic conditions in vivo, the objective of this study was to investigate the effect of culture temperature on the proliferation and differentiation of pig preadipocytes in primary culture. Culturing primary preadipocytes at 37 rather than 39°C decreases their proliferation rates based upon cleavage of the tetrazolium salt, MTT (P < 0.001), reduction of resazurin (P < 0.001), and daily cell counts (P < 0.001). Likewise, culturing primary porcine preadipocytes at 37°C suppressed their adipogenic potential based upon monitoring adipogenesis morphologically, biochemically, and via the expression of mRNA encoding adipogenic marker genes. Collectively, these data indicate the proliferation and differentiation of primary pig preadipocytes is suppressed when cultures are incubated at 37°C compared to normal body temperature of pigs. This may confound investigation of factors that impact adipocyte hyperplasia in the pig.

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“…More attention has however been paid to the adipose tissue from a proteomic perspective. In this sense, differences among fat-type piglets compared to intensively lean selected breed pigs have been noted in sub-cutaneous fat tissues regarding up-regulation of the lipogenic enzymes (malic enzyme and glucose-6-phosphate dehydrogenase), carboxylesterase-1, serpins and low-grade inflammation markers (interleukin-6 and tumour necrosis factor ␣) [36,37]. Adipose tissue has been shown to have different metabolic activity depending on its location.…”

Section: Obesitymentioning

confidence: 99%

The pig as an animal model for human pathologies: A proteomics perspective

Bassols

Costa

Eckersall

et al. 2014

Proteomics Clinical Apps

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Traditional biomedical models are easy to manage in experimental facilities and allow fast and affordable basic genetic studies related to human disorders, but in some cases they do not always represent the complexity of their physiology. Translational medicine demands selected models depending on the particularities of the human disease to be investigated, reproducing as closely as possible the evolution, clinical symptoms and molecular pathways, cells or tissues involved in the dysfunction. Thus, pig models offer an alternative because of their anatomical and physiological similarities to humans and the availability of genomic, transcriptomic and, progressively more, proteomic tools for analysis of this species. Furthermore, there is a wide range of natural, selected and transgenic porcine breeds. The present review provides a summary of the applications of the pig as a model for metabolic, cardiovascular, infectious diseases, xenotransplantation and neurological disorders and an overview of the possibilities that the diverse proteomic techniques offer to study these pathologies in depth.

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A comparison of subcutaneous adipose tissue proteomes in juvenile piglets with a contrasted adiposity underscored similarities with human obesity

Cited by 17 publications

References 46 publications

Single-Nucleotide Polymorphisms in PigEPHX1Gene are Associated with Pork Quality Traits

Single-Nucleotide Polymorphisms in PigEPHX1Gene are Associated with Pork Quality Traits

The proliferation and differentiation of primary pig preadipocytes is suppressed when cultures are incubated at 37°Celsius compared to euthermic conditions in pigs

The pig as an animal model for human pathologies: A proteomics perspective

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