A comparison of the biological activities of the cell-adhesive proteins vitronectin and fibronectin

Abstract

The effects of vitronectin and fibronectin upon the attachment and growth of bovine corneal endothelial cells (BCE) and BHK-21 cells were compared. Similar dose-response curves for cell attachment to the substratum were obtained for both molecules and both cell types, although BCE cells exhibited a slight preference for vitronectin, and BHK cells for fibronectin. When, however, cells were plated in medium containing bovine serum stripped of fibronectin, they attached and grew normally, whereas in medium containing serum stripped of vitronectin, cells either failed to attach (BHK-21) or attached but exhibited poor cell spreading and growth. This dependence of cells upon vitronectin, rather than fibronectin, in serum for cell attachment, was shown to be due to a failure of fibronectin to coat the substratum in the presence of other serum proteins. Vitronectin was able to coat the substratum efficiently in the presence of other serum proteins. Although dependent upon vitronectin for adhesion to the substratum, bovine endothelial cells were unable to synthesize endogenous vitronectin.

Surface-modified 3D starch-based scaffold for improved endothelialization for bone tissue engineering

“…However, this is not surprising, as it is reported that in complex protein solutions Fn interacts with the available cell non-adhesive molecules, 53 which reduces its competitiveness and explains its failure to adsorb on to the substrate in the presence of other serum proteins. 54 As a result, in bovine plasma and fresh serum higher cell activity is associated with Vn but not with Fn.…”

Adsorption of vitronectin, collagen and immunoglobulin-G to plasma polymer surfaces by enzyme linked immunosorbent assay (ELISA)

“…Vitronectin has been implicated in the attachment of a number of cell types, including human endothelial cells, 5 to tissue culture surfaces. This may explain to some extent, other data showing high levels of cell adhesion to acid-containing plasma deposited surfaces in preference to other plasma polymers.…”

“…The adsorption of adhesive proteins such as fibronectin and vitronectin has been shown to be of great importance when culturing a variety of cells in vitro. [5][6][7] Plasma polymerisation is a promising route to fabrication of surfaces with diverse properties. 8 In plasma polymerisation a thin (v 100 nm) film may be deposited onto substrates of complex geometry, modifying the chemistry of the surface region.…”

Nature-Derived and Synthetic Additives to poly(ɛ-Caprolactone) Nanofibrous Systems for Biomedicine; an Updated Overview

“…One important reason is the increased protein adsorption on the surfaces with moderate hydrophilicity ( Lee et al, 1998 ). The improved adsorption of cell-adhesive serum proteins such as vitronectin and fibronectin can highly affect cell adhesion, growth, and morphology ( van Wachem et al, 1987 ; Underwood and Bennett, 1989 ; Steele et al, 1995 ). PCL nanofibers are inherently hydrophobic and do not expose polar functional groups on the surface.…”