A comparison of the capacity of six cold-pressed plant oils to support development of acquired immune competence in the weanling mouse: superiority of low-linoleic-acid oils

Hillyer, Lyn M.; Woodward, Bill

doi:10.1079/bjnbjn2002602

Cited by 2 publications

(8 citation statements)

References 40 publications

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“…Food intake and final body weight were not affected by dietary oil source (P¼0•57 and P¼0•29, respectively), and weight change was unapparent during the 8-week experimental period (Student's t test, P¼0•19). The fatty acid composition expected of the oil sources was confirmed by chemical analysis (results not shown) and was similar to results reported previously (Hillyer & Woodward, 2002). In turn, the serum n-3 and n-6 fatty acid profiles of the test groups reflected the composition of their respective oil sources.…”

Section: Resultssupporting

confidence: 89%

“…cold-pressed flaxseed or safflower oils (Omegaflo w ), provided as a gift from Omega Nutrition Canada, Inc. (Vancouver, BC, Canada). These diets are described elsewhere (Hillyer & Woodward, 2002) and contained 80 g of their respective oil source per kg. While on experiment, the mice were caged individually, had free access to diet and tap water, and were permitted coprophagy.…”

Section: Animals Diets and Animal Facilitymentioning

confidence: 99%

“…A blood sample from the orbital plexus was taken from each test mouse at the end of the experimental period (day 56) and also from each mouse of the two control groups. The procedure including storage of serum samples is described elsewhere (Hillyer & Woodward, 2002).…”

Section: Blood Sampling and Serum Storagementioning

confidence: 99%

“…Fatty acid analyses of serum and dietary oils were performed by capillary GC as described elsewhere (Hillyer & Woodward, 2002). Likewise, the procedures used to determine the DM and lipid contents of the animal carcasses are outlined elsewhere (Hillyer & Woodward, 2002).…”

Section: Chemical Analyses Of Serum Dietary Oils and Carcassesmentioning

confidence: 99%

“…Humoral competence was assessed as the primary antibody response to the sheep red blood cell (SRBC; CedarLane, Hornby, ON, Canada) according to a published procedure (Hillyer & Woodward, 2002). The immunizing dose of SRBC, or the negative control injection of saline carrier, was given to mice of the test groups on day 51 of the experimental period and serum agglutinin titre (i.e.…”

Section: Assessment Of Humoral Immune Competencementioning

confidence: 99%

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Cold-pressed flaxseed oil reverses age-associated depression in a primary cell-mediated adaptive immune response in the mouse

et al. 2006

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The objective of this investigation was to determine the influence of flaxseed oil on responses representative of primary humoral and cell-mediated adaptive immune competence in immunosenescent mice. Male and female C57BL/6J mice, 85 weeks old, were randomized between two complete purified diets differing only in oil source (cold-pressed safflower or flaxseed). After 8 weeks, humoral competence was assessed in six mice per group as the serum haemagglutinin titre to sheep red blood cells (SRBC) and cell-mediated competence was assessed, in an additional six mice per group, as the delayed hypersensitivity response to SRBC. A zero-time control group (88 weeks old) and a young adult positive control group (12 weeks old) were each tested similarly (six per immune response), revealing age-related depression in both antibody and cell-mediated competence at 88 weeks of age. After the 8-week experimental period, the antibody response of the two test groups of geriatric mice remained below the young adult level (P¼ 0·04) and the cell-mediated response of the safflower oil group also continued to exhibit age-related depression (20 % of young adult level, P¼0·0002). By contrast, the anti-SRBC delayed hypersensitivity response of the flaxseed group no longer differed from the response of the young adults but exceeded that of the safflower and zero-time control senescent groups (P¼ 0·0002). Depression in primary cell-mediated competence, the most outstanding aspect of immunosenescence, can be addressed by means of a dietary source of 18 : 3n-3 without longer-chain PUFA.

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Section: Resultssupporting

confidence: 89%

Section: Animals Diets and Animal Facilitymentioning

confidence: 99%

Section: Blood Sampling and Serum Storagementioning

confidence: 99%

Section: Chemical Analyses Of Serum Dietary Oils and Carcassesmentioning

confidence: 99%

Section: Assessment Of Humoral Immune Competencementioning

confidence: 99%

See 3 more Smart Citations

Cold-pressed flaxseed oil reverses age-associated depression in a primary cell-mediated adaptive immune response in the mouse

et al. 2006

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Mouse Chow Composition Influences Immune Responses and Food Allergy Development in a Mouse Model

et al. 2018

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Our diet is known to substantially influence the immune response not only by support of mucosal barriers but also via direct impact on immune cells. Thus, it was of great interest to compare the immunological effect of two mouse chows with substantial differences regarding micro-, macronutrient, lipid and vitamin content on the food allergic response in our previously established mouse model. As the two mouse chows of interest, we used a soy containing feed with lower fatty acid (FA) amount (soy-containing feed) and compared it to a soy free mouse chow (soy-free feed) in an established protocol of oral immunizations with Ovalbumin (OVA) under gastric acid suppression. In the animals receiving soy-containing feed, OVA-specific IgE, IgG1, IgG2a antibody levels were significantly elevated and food allergy was evidenced by a drop of body temperature after oral immunizations. In contrast, mice on soy-free diet had significantly higher levels of IL-10 and were protected from food allergy development. In conclusion, soy-containing feed was auxiliary during sensitizations, while soy-free feed supported oral tolerance development and food allergy prevention.

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A comparison of the capacity of six cold-pressed plant oils to support development of acquired immune competence in the weanling mouse: superiority of low-linoleic-acid oils

Cited by 2 publications

References 40 publications

Cold-pressed flaxseed oil reverses age-associated depression in a primary cell-mediated adaptive immune response in the mouse

Cold-pressed flaxseed oil reverses age-associated depression in a primary cell-mediated adaptive immune response in the mouse

Mouse Chow Composition Influences Immune Responses and Food Allergy Development in a Mouse Model

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