A comparison of the collagen triple helix and coiled‐coil peptide building blocks on metal ion‐mediated supramolecular assembly

Abstract

Metal ion-mediated assembly of peptides is an intriguing method for developing novel biomaterials. In particular, peptide building blocks based on collagen triple helical and coiled-coil peptides have been functionalized with metal-binding ligands and assemble into hierarchical structures with diverse potential applications. This review outlines the use of metal-mediated assembly with these supramolecular structures and highlights how changes in the building blocks lead to significant differences in structural morphologies, with the identity of the metal-binding ligands and their placement on the building block as crucial aspects in the bottom-up development of the assemblies.

Recent Advances in Collagen Mimetic Peptide Structure and Design

“…Since higher order assemblies of triple helices frequently utilize freetermini charges for assembly, these results are also of importance for biomaterial design. [10][11][12]14 The organization of the triple helix formed by collagen mimetic peptides was shown through selectively labeling triplets from the N-to C-terminus. 59 NMR on this series of (POG) 10 homotrimers showed the core to be well folded while the two terminal triplets on both the N-and C-termini were disordered with fraying or slight unfolding (Figure 6B).…”

“…These different termini-functionalities are therefore important to consider when predicting CMP stabilities. Since higher order assemblies of triple helices frequently utilize free-termini charges for assembly, these results are also of importance for biomaterial design. − , …”

“…Another application of great interest is CMPs in self-assembled biomaterials. (See refs − for recent reviews.) 2D nanoscale assemblies from CMPs containing blocks of positive and negative charges have been demonstrated .…”

“…Temperature prediction algorithms as a tool for designing heterotrimers will be explored, as well as the use of CMPs to study natural collagen sequences and diseases. Topics such as collagen targeting, , protein interactions with collagen, − higher order assemblies of CMPs, − collagen biomaterials, ,, and peptide–polymer conjugates − have been reviewed recently and are therefore outside the scope of this Perspective.…”

Formation of Microcages from a Collagen Mimetic Peptide via Metal-Ligand Interactions

“…The use of metal-ligand interactions as a means to prompt the assembly of CMPs has also been extensively explored [ 2 , 26 , 27 ]. The placement of ligands at the termini of triple helices has generated microflorettes [ 28 , 29 ], spiraled-horn networks [ 30 ], nanoropes [ 31 ], and petal-like structures [ 32 , 33 ] in the presence of metal ions, the latter of which demonstrate nanoscale banding, a behavior found within natural collagen.…”