A comparison of the effects of N-acetylcysteine and ethyl pyruvate on experimental testicular ischemia-reperfusion injury

Turkmen, Süha; Menteşe, Ahmet; Karagüzel, Ersagun; Karaca, Yunus; Küçük, Adem; Uzun, Aysegul; Yuluğ, Esin; Türedi, Süleyman

doi:10.1016/j.fertnstert.2012.05.034

Cited by 45 publications

(23 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Testicular T/D is considered as a typical I/R injury for the testis, associating with the overgeneration of reactive oxygen species (ROS), which has destructive effects on a series of cellular functions . MDA, a stable end product of lipid peroxidation that is generated by ROS, is widely used as an indirect indicator of ROS, which has high reactivity and short half‐life.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Uncultured Adipose-Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion-Detorsion in Rats

Zhou

Song

et al. 2018

Stem Cells Translational Medicine

View full text Add to dashboard Cite

Torsion‐detorsion (T/D)‐induced testicular injury may lead to male subfertility and even infertility. Stem cell therapy provides an alternative to attenuate testicular injury and promote spermatogenesis. Adipose‐derived stromal vascular fraction (SVF) can be acquired conveniently without in vitro expansion, which may avoid the potential risks of microbial contamination, xenogenic nutritional sources, etc., during cell culture. In this study, we investigate the protective effects of autologous uncultured SVF on testicular injury and spermatogenesis in a rat model of T/D. Animals were randomly divided into sham, T/D+ phosphate‐buffered saline, and T/D + SVF groups (18 rats in each group). SVF was isolated, labeled with lipophilic fluorochrome chloromethylbenzamido dialkylcarbocyanine, and transplanted into T/D testis by local injection. At 3, 7, 14, and 28 days F surgery, testicular tissue and serum samples were harvested for histopathological, immunohistochemical, Western blot, and enzyme‐linked immunosorbent assays. Histopathological findings demonstrated severe injury in the testis with decreased Johnsen's score led by T/D, while uncultured SVF reduced testicular injury and elevated the decreased score. Injected SVF cells were mainly integrated into interstitial region and seminiferous tubules, enhanced the secretion of basic fibroblast growth factor and stem cell factor in the testis, contributed to the declining level of malondialdehyde and restoration of hormonal homeostasis, and then reduced the injury of Leydig cells and germ cells, as well as promoting spermatogenesis. Our findings demonstrated that autologous uncultured SVF could protect the testis from testicular ischemia‐reperfusion injury and promote spermatogenesis, which provide significant clinical implications for the prevention of infertility induced by testicular T/D. Stem Cells Translational Medicine 2019;8:383–391

show abstract

Section: Discussionmentioning

confidence: 99%

Protective Effects of Uncultured Adipose-Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion-Detorsion in Rats

Zhou

Song

et al. 2018

Stem Cells Translational Medicine

View full text Add to dashboard Cite

show abstract

“…A longer reperfusion process is needed for penile ischemic products to exhibit systemic effects and for significant changes to be identified in ischemic parameters in serum. This study represents the first investigation in a priapism model of IMA, a biochemical parameter that has been investigated and found to be of value in such different models of I/R injury as testicular torsion ( 30 , 31 ).…”

Section: Discussionmentioning

confidence: 99%

The possible protective effects of dipyridamole on ischemic reperfusion injury of priapism

et al. 2016

Self Cite

View full text Add to dashboard Cite

Purpose To investigate the protective effects against ischemia reperfusion injury of dipyridamole in a model of induced priapism in rats.Materials and Methods Twenty-four male Sprague-Dawley rats were divided into four groups, control, P/R, P/R+DMSO and P/R+D. 3ml blood specimens were collected from vena cava inferior in order to determine serum MDA, IMA, TAS, TOS and OSI values, and penile tissue was taken for histopathological examination in control group. Priapism was induced in P/R group. After 1h, priapism was concluded and 30 min reperfusion was performed. In P/R+DMSO group 1ml/kg DMSO was administered intraperitoneally 30 min before reperfusion, while in P/R+D group 10mg/kg dipyridamole was administered intraperitoneally 30 min before reperfusion. Blood and penis specimens were collected after the end of 30 min reperfusion period. Sinusoidal area (µm2), tears in tunica albuginea and injury parameters in sinusoidal endothelium of penis were investigated.Results Histopathological examination revealed no significant changes in term of sinusoidal area. A decrease in tears was observed in P/R+D group compared to P/R group (p<0.05). Endothelial injury decreased in P/R+D group compared to P/R group (p>0.05). There were no significant differences in MDA and IMA values between groups. A significant increase in TOS and OSI values was observed in P/R+D group compared to P/R group. A significant decrease in TAS levels was observed in P/R+D group compared to the P/R group.Conclusions The administration of dipyridamole before reperfusion in ischemic priapism model has a potential protective effect against histopathological injury of the penis.

show abstract

“…Turkmen et al[100] reported that ethyl pyruvate has a positive effect on torsion-detorsion associated I/R injury in an experimental rat model.…”

Section: Searchmentioning

confidence: 99%

Antioxidants in experimental ischemia-reperfusion injury of the testis: Where are we heading towards?

Vaos

Zavras

2017

WJM

View full text Add to dashboard Cite

Testicular torsion (TT) is a medical emergency that primary affects newborns and young adolescents. It causes testicular injury due to the torsion of the spermatic cord and its components, initially in the venous blood flow and finally in the arterial blood flow. Prompt diagnosis and early surgical management are necessary in managing this urgent situation. The process of the pathophysiological events in ischemia-reperfusion is multifactorial and deals with the perception of the oxidative stress responsible for the consequences of ischemia/reperfusion (I/R) stress following TT. Duration and severity of torsion also play a significant role in the oxidative stress. A detrimental result of the defense system of the testes takes place resulting finally in testicular atrophy and impaired function. Antioxidant factors have been experimentally studied in an effort to front this state. They have been classified as endogenous or exogenous antioxidants. Endogenous antioxidants comprise a structure of enzymic enzymatic and non-enzymic enzymatic particles presented within cytoplasm and numerous other subunits in the cells. Exogenous antioxidants include a variety of natural and pharmaceutical agents that may prevent or ameliorate the harmful effects of I/R injury. In this study we review those factors and their ability to enhance the oxidative status of the testis. A feature insight into where we are heading is attempted.

show abstract

A comparison of the effects of N-acetylcysteine and ethyl pyruvate on experimental testicular ischemia-reperfusion injury

Cited by 45 publications

References 24 publications

Protective Effects of Uncultured Adipose-Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion-Detorsion in Rats

Protective Effects of Uncultured Adipose-Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion-Detorsion in Rats

The possible protective effects of dipyridamole on ischemic reperfusion injury of priapism

Antioxidants in experimental ischemia-reperfusion injury of the testis: Where are we heading towards?

Contact Info

Product

Resources

About