A comparison of the effects upon HeLa cells of isopropyl methane sulphonate and X-rays during different phases of the cell cycle

Petrović, Danilo; Nias, A. H. W.

doi:10.1016/0014-2964(67)90014-x

Cited by 11 publications

(2 citation statements)

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“…2 where a 15 min treatment with MMS 100 pg/ml was given to aliquots of synchronized cell populations at intervals during the 27 hr following mitotic selection. Pulse labelling of aliquots of this population confirmed the labelling pattern shown previously (Petrovic & Nias, 1967) to delimit the GI period to the first 11 hr; the S period follows continuing up to 22 hr. The G2 period then begins, but mitosis and the second G1 period tend to overlap in the population which tends to lose synchrony by this time.…”

Section: Results (I) Increased Drug Sensitiuitysupporting

confidence: 84%

“…6 where populations of normal and M4 HeLa cells are again compared. The observations begin at 10 hr towards the end of the GI phase of normal HeLa cells and show the S phase pattern of these cells as previously shown by Petrovic & Nias (1967) pattern, which was shown in Fig. 5 to be similar to that of the normal HeLa cells, but at a later time.…”

Section: (3) Phase Distribution Of Clone M4supporting

confidence: 81%

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KINETICS OF A DRUG SENSITIVE CLONE OF HeLa CELLS

Nias

Fox

1970

Cell Proliferation

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Drug sensitive clones have been isolated from a population of HeLa cells after treatment with methyl methane sulphonate (MMS). One of these clones has now been studied in more detail and some possible mechanisms of drug sensitivity have been related to the proliferation kinetics of the cells of this clone. The cell cycle is elongated and labelling techniques show this elongation to be confined to the G1 phase. Synchronized cultures have shown that the G1 phase is more sensitive to MMS than the S phase. These observations provide some explanation for the drug sensitivity of this clone of cells.

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Section: Results (I) Increased Drug Sensitiuitysupporting

confidence: 84%

Section: (3) Phase Distribution Of Clone M4supporting

confidence: 81%