A comparison of the efficiency of Rappaport-Vassiliadis, tetrathionate and selenite broths with and without pre-enrichment for the isolation of salmonella in animal waste biogas plants

Schlundt, Jørgen; Munch, Bente

doi:10.1016/s0934-8840(11)80366-x

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…RV was selected for enrichment based on cost and its reported higher recovery rates for Salmonella spp. compared to selenite and tetrathionate broths, even without preenrichment [16]. A sterile swab from raw or processed milk sample was inoculated into RV and incubated at 37°C for 24 hours.…”

Section: Methodsmentioning

confidence: 99%

Detection ofSalmonellaspp.,Candida albicans,Aspergillusspp., and Antimicrobial Residues in Raw and Processed Cow Milk from Selected Smallholder Farms of Zimbabwe

Mhone

Matope

Saidi

2012

Veterinary Medicine International

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A cross-sectional study was conducted to detect the presence of Salmonella spp., Candida albicans, Aspergillus spp., and antimicrobial residues in raw milk (n = 120) and processed cow milk (n = 20) from smallholder dairy farms from three sites in Zimbabwe. Culture and isolation of Salmonella spp., C. albicans, and Aspergillus spp. were performed using selective media, while antimicrobial residues were detected by a dye reduction test. No Salmonella, but C. albicans (17.5%; 21/120), Aspergillus spp. (0.8%; 1/120), and antimicrobial residues (2.5%; 3/120) were detected from raw milk. C. albicans was isolated from all three sites, while Aspergillus spp. and antimicrobial residues were detected from sites 1 and 3, respectively. From processed milk, only C. albicans (5%) was isolated while Aspergillus spp. and antimicrobial residues were not detected. These results suggested low prevalence of Salmonella spp. and Aspergillus spp. and a relatively high prevalence of C. albicans in raw milk from the smallholder farms. The potential public health risks of C. albicans and the detected antimicrobial residues need to be considered. Thus, educating farmers on improving milking hygiene and storage of milk and establishing programmes for monitoring antimicrobial residues may help to improve the safety of milk from smallholder farms.

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Section: Methodsmentioning

confidence: 99%

Detection ofSalmonellaspp.,Candida albicans,Aspergillusspp., and Antimicrobial Residues in Raw and Processed Cow Milk from Selected Smallholder Farms of Zimbabwe

Mhone

Matope

Saidi

2012

Veterinary Medicine International

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“…Hence, the a priori expected value and standard deviation were E( p f ) = 0.92 and Std( p f ) = 0.03 derived from salmonella studies with fecal matrix. (12,13) …”

Section: Conditional Probability Of a Herd To Be Detected When Testedmentioning

confidence: 99%

Estimation of True Salmonella Prevalence Jointly in Cattle Herd and Animal Populations Using Bayesian Hierarchical Modeling

Ranta¹,

Tuominen

Maijala

2005

Risk Analysis

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The Finnish salmonella control program (FSCP) for beef production is based on both randomized and selective testing of herds and animals. Sampling of individual animals in abattoirs is randomized. Herds are selectively tested for salmonella on the basis of clinical symptoms and/or other factors. Risk assessment of FSCP is inherently difficult due to the complexity of the complete data set, especially if the detailed labeling of the testing types is lost. However, for a risk assessment of the whole production chain, methods for exploiting all available data should be considered. For this purpose, a hierarchical Bayesian model of true salmonella prevalence was constructed to combine information at different levels of aggregation: herds in geographical regions and individual animals arriving for slaughter. The conditional (municipality specific) probability of selection of a herd for testing was modeled given the underlying true infection status of the herd and information about the general sampling activity in the specific region. The model also accounted for the overall sensitivity of the sampling methods, both at the herd and at the animal level. In 1999, the 95% posterior probability intervals of true salmonella prevalence in the herd population, in individual cattle, and in slaughter animal populations were [0.54%, 1.4%] (mode 0.8%), [0.15%, 0.39%] (mode 0.2%), and [0.12%, 0.36%] (mode 0.2%), respectively. The results will be further exploited in other risk assessments focusing on the subsequent parts of the beef production chain, and in evaluation of the FSCP.

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“…After selective enrichment, three selective agars are applied in each method. All reported studies on the comparison of isolation methods for Salmonella used either the FDA/BAM or USDA/FSIS methods as the standard or control (Schlundt and Munch 1993; Read et al. 1994; Warburton et al.…”

Section: Introductionmentioning

confidence: 99%

AN IMPROVED METHOD FOR RAPID ISOLATION OF SALMONELLA AGAINST PROTEUS IN CHICKEN CARCASSES

Cui

Zheng

Meng

2006

Journal of Food Safety

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An improved method was developed for the rapid isolation and identification of Salmonella in chicken carcasses. Considering the heavy presence of Proteus mirabilis in the poultry‐production environment, we investigated combinations of selective bacterial growth media that promote the growth of Salmonella but inhibit that of P. mirabilis. The improved method was evaluated using standard methods described in the Bacteriological Analytical Manual (BAM) by the U.S. Food and Drug Administration (FDA), and the Microbiology Laboratory Guidebook published by the U.S. Department of Agriculture Food Safety Inspection Service (USDA/FSIS). A total of 44 chicken carcasses were analyzed for Salmonella using the three methods. The FDA/BAM method showed a poor sensitivity and specificity, especially when the tetrathionate (TT) broth was applied. The improved method offered an excellent specificity (>95%) and shortened the time of isolation and identification from 4 to 5 days to less than 3 days, consequently reducing the costs.

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A comparison of the efficiency of Rappaport-Vassiliadis, tetrathionate and selenite broths with and without pre-enrichment for the isolation of salmonella in animal waste biogas plants

Cited by 4 publications

References 13 publications

Detection ofSalmonellaspp.,Candida albicans,Aspergillusspp., and Antimicrobial Residues in Raw and Processed Cow Milk from Selected Smallholder Farms of Zimbabwe

Detection ofSalmonellaspp.,Candida albicans,Aspergillusspp., and Antimicrobial Residues in Raw and Processed Cow Milk from Selected Smallholder Farms of Zimbabwe

Estimation of True Salmonella Prevalence Jointly in Cattle Herd and Animal Populations Using Bayesian Hierarchical Modeling

AN IMPROVED METHOD FOR RAPID ISOLATION OF SALMONELLA AGAINST PROTEUS IN CHICKEN CARCASSES

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