A Comparison of the Flora of the Comedones of Acne Vulgaris and Comedones in Elderly People

Ganor, S.; Sacks, T.

doi:10.1159/000253959

Cited by 24 publications

(11 citation statements)

References 10 publications

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“…The investigators failed to culture all the corynebacteria identified microscopically and, therefore, corynebacteria from only 20% of the acne comedones were identified as C. acnes . Similarly, staphylococci from only 41% of the comedones were identified as S. albus 20 . No significant difference was found in the incidence of staphylococci or Pityrosporum spp.…”

Section: Microbiology Of Acne Lesionsmentioning

confidence: 89%

“…Likewise, S. albus was isolated from 96% of the lesions while none of the comedones was found to be sterile. Similarly, Ganor and Sacks 20 compared the microbial flora of senile and acne comedones and found corynebacteria (synonymous with propionibacteria) and staphylococci in 65% and 51% of the acne comedones, respectively. Moreover, yeasts were seen in 67% of these lesions.…”

Section: Microbiology Of Acne Lesionsmentioning

confidence: 99%

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A microbial aetiology of acne: what is the evidence?

Shaheen

González

2011

British Journal of Dermatology

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A microbial aetiology of acne has been suggested since the beginning of the last century. There is considerable evidence, circumstantial at best, which suggests that micro-organisms, particularly Propionibacterium acnes, are important in the pathogenesis of acne vulgaris. However, it is still unclear whether P. acnes is actually a causal agent in the development of noninflamed or inflamed acne lesions. Based on a review of the microbiological data on normal and acne-affected skin, we propose that P. acnes neither initiates comedogenesis nor has a role in the initiation of inflammation in inflamed acne lesions.

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Section: Microbiology Of Acne Lesionsmentioning

confidence: 89%

Section: Microbiology Of Acne Lesionsmentioning

confidence: 99%

A microbial aetiology of acne: what is the evidence?

Shaheen

González

2011

British Journal of Dermatology

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“…One of the main reasons for the ambiguity was the lack of suitable culture media for quantitative recovery of Pityrosporum, particularly P. orbiculare (7,12,14).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, a quantitative analysis of the number of Pityrosporum in specimens from normal or diseased sites of the human skin was usually performed by direct microscopic counting of the cells. A differentiation of P. ovale and P. orbiculare was made by comparing morphological characteristics (7,12,14).…”

mentioning

confidence: 99%

Selective and Differential Media for Isolation and Tentative Identification of Each Species of Pityrosporum Residing on Normal or Diseased Human Skin

Ushijima

Takahashi

Ozaki

1981

Microbiology and Immunology

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Selective and differential media were designed for each species of Pityrosporum ; P. pachydermatis, P. ouale, and P. orbiculare in order to make feasible a quantitative cultivation.Medium for P. pachydermatis (medium A) was composed of 1% trypticase peptone (BBL), 0.5% yeast extract (BBL), 0.3% glucose, 0.2% NaCl, 1.2% KH2 P04 (anhydrous), 1.5% agar, 0.01% ampicillin, and 0.025% cycloheximide with a pH of 5.5. Medium for P. ouale (medium B) was medium A supplemented with 0.05% sodium acetate (anhydrous), 0.2% Tween 80, and 0.025% (selective medium) or 0.075% (differential medium) sodium laurate. Medium for P. orbiculare was medium B (devoid of laurate) supplemented with 2% olive oil, 0.25% glycerol, 0.25% gall powder, 0.05% sodium palmitate, 0.05% sodium stearate, 0.05% sodium oleate and 8% (selective medium) or 10% (differential medium) sodium lactate and an increase in Tween to 1%.For isolation of Pityrosporum, specimens were suspended in 0.1% Tween 80 solution and inoculated onto agar plates of three selective media. The plates were incubated aerobically at 37 C for 8-10 days under conditions of prevention of water loss from the media. The plating efficiency of each selective medium, expressed as a ratio of cultural counts to microscopic counts was generally over 70%. Species of Pityrosporum could also be identified when we inoculated the cell suspension onto differential agar plates and incubated the preparations at 37 C for 7 days.

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“…The reports since then are limited and are concerned mainly with Pit. ovule in non-inflamed lesions (Ganor & Sacks 1969;Marples et al 1973;Wolff & Plewig 1976;Wilborn et al 1978) where this organism is a common inhabitant. In all acne lesions P. acnes is quantitatively the most important.…”

Section: Quantitative Studies Of Acne Lesionsmentioning

confidence: 99%