A comparison of the inhibitory effects of disodium pyrophosphate and disodium ethane‐hydroxy‐1, 1‐diphosphonate on simple calcergy

1982

The effects of high doses of EHDP on the local Pb-acetate induced calcergy were studied in young female rats. A plaque of hydroxyapatite crystals was produced by local subctaneous injection of a lead acetate dilution. Reactions at injection site of dilution were evaluated by the mean diameter and the dry weight of the calcified wheals. At high doses of EHDP (above 20mg/kg/day), the hydroxyapatite crystal deposition were inhibited significantly.

Section: 2560supporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: 2560mentioning

confidence: 99%

See 1 more Smart Citation

The effects of disodium ethane‐hydroxy‐ 1, 1‐diphosphonate and disodium dichloromethylene diphosphonate on lanthanide‐induced calcergy

1982

“…It inhibits mineralization of growth cartilage and metaph seal osteoid in rats," in cutaneous" and cardiac' calciphylaxis, in calcergy induced by potassium permanganate (KMn04) in the rat'4 and calcergy induced by lead acetate (PbAc) in the mouse . 5 The present experiments confirm the efficacy of EHDP in inhibiting cutaneous calciphylaxis but only when administered either before or before and after the initiating experimental procedure.…”

Section: Discussionsupporting

confidence: 67%

Cutaneous calciphylactic reactions in the mouse and the rat and the effects of diphosphonates on the reaction in the rat

Miller

Vernon-Roberts

1984

Self Cite

Calciphylaxis is a local tissue calcific reaction at the site of an injection of challenger substance given a critical time period after the oral administration of a sensitizer substance such as dihydrotachysterol (DHT), vitamin D or parathormone. Cutaneous calciphylaxis is readily induced in the rat but not in the mouse and this may be because, in the latter, the challenger substance is absorbed rapidly by macrophages. In the rat the administration of 500 micrograms/0.1 ml of DHT followed after 24 h by the subcutaneous (SC) injection of ferric chloride (FeCl3) (30 micrograms/0.1 ml) is followed rapidly by calcification of the SC site. There is an early transient acute inflammatory reaction with the incrustation of collagen fibres by the iron salt and an apparent exudation of calcium and phosphate ions from the bloodstream. These ions also become associated with collagen fibres. Two days after injection macrophages and multinucleated giant cells become the dominant cells. Calciphylaxis is a useful experimental model of ectopic calcification and is associated with an initial hypercalcaemia. The diphosphonates ethane-1-hydroxy-1, 1-diphosphonate (EHDP) and dichloromethylene diphosphonate (Cl2MDP) are effective inhibitors of the calciphylactic reaction when administered prior to the initiation of the experimental procedure.

“…Secondly, they provide systems whereby substances known or thought to influence calcification reactions may be tested for such an effect. The calcergenic effects of lead acetate and potassium permanganate have been utilised by McClure (1979) and Doyle, Dunn and Willoughby (1979) respectively for this latter purpose. The reactions produced by these two chemicals are circular in outline and therefore the response may be quantitated by taking the mean of the diameter of the lesions measured in two places.…”

mentioning

confidence: 99%

Lanthanide‐induced calcergy

Garrett

1981

Self Cite

The subcutaneous injection of solutions of chloride salts of the rare earths is followed by calcification of the dorsal fascia in the mouse. The concentration of salt required to produce a constant, predictable response varies from salt to salt. The macroscopic outline of the reaction is generally irregular and therefore a quantitative radiological method has been developed to allow an accurate assessment of the dose/response relationship. This with the majority of salts is best described as a log regression and with three as a linear regression.