A Comparison of the Limits of Detection for a Number of Surrogates of Organophosphorus Toxic Agents and Methylphosphonic Acid Silyl Derivatives and its O-Alkyl Esters by Gas Chromatography/Mass Spectrometry with Various Ionization Methods and a Flameless Thermionic Ionization Detector

Yashin, Yu. S.; Revelsky, I. A.; Тихонова, И. Н.; Karavaeva, Veronika G.; Virus, Edward Danielevich; Chepelyansky, D. A.; Revelsky, A. I.

doi:10.1134/s1061934820130146

Cited by 1 publication

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“…Currently, mass spectrometry (MS) and gas chromatography (GC) techniques are the most common methods for nerve agent confirmation. ,,− MS methods are the most highly sensitive, with reported detection limits on the order of 10–50 nM (1–4 ng/mL). , While MS and GC methods are powerful and sensitive techniques, they require intricate and time-consuming sample preparation and analyses. The presence of interfering molecules has also been shown to be detrimental to detection limits.…”

Section: Introductionmentioning

confidence: 99%

Quantum Dot–DNA FRET Conjugates for Direct Analysis of Methylphosphonic Acid in Complex Media

et al. 2023

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Rapid detection of nerve agents from complex matrices with minimal sample preparation is essential due to their high toxicity and bioavailability. In this work, quantum dots (QDs) were functionalized with oligonucleotide aptamers that specifically targeted a nerve agent metabolite, methylphosphonic acid (MePA). These QD-DNA bioconjugates were covalently linked to quencher molecules to form Förster resonance energy transfer (FRET) donor–acceptor pairs that quantitatively measure the presence of MePA. Using the FRET biosensor, the MePA limit of detection was 743 nM in artificial urine. A decrease in the QD lifetime was measured upon DNA binding and was recovered with MePA. The biosensor’s flexible design makes it a strong candidate for the rapid detection of chemical and biological agents for deployable, in-field detectors.

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