A comparison of the non-specific acid phosphomonoesterase activity in the larva ofPhocanema decipiens (Nematoda) with that of the muscle of its host the codfish (Gadus morhua)

Goil, Madan M.; Harpur, R. P.

doi:10.1007/bf00927973

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…Origin of phosphomonoesterase was shown to affect its Michaelis-Menten characteristics ( K m and V max⁡ values) and response to pollutants (e.g., Cu) and other compounds in soil [30, 31]. Also, Gould et al [9] reported that properties of microbe- and root-derived EAPM were different including their kinetic parameters and temperature sensitivity.…”

Section: Resultsmentioning

confidence: 99%

Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems

Rejšek

Vranová

Formánek

2012

The Scientific World Journal

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The aim of this study is to present a new method for determining the root-derived extracellular acid phosphomonoesterase (EAPM) activity fraction within the total EAPM activity of soil. EAPM activity was determined for roots, organic and mineral soil. Samples were collected using paired PVC cylinders, inserted to a depth of 15 cm, within seven selected forest stands. Root-derived EAPM formed between 4 and18% of the total EAPM activity of soil from forests of differing maturity. A new approach, presented in this work, enables separation of root-derived EAPM activity from total soil EAPM. Separation of root-derived EAPM from soil provides a better understanding of its role in P-cycling in terrestrial ecosystems. The method presented in this work is a first step towards the separation of root- and microbe-derived EAPM in soils, which are thought to possess different kinetic properties and different sensitivity to environmental change.

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Section: Resultsmentioning

confidence: 99%

Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems

Rejšek

Vranová

Formánek

2012

The Scientific World Journal

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“…Heavy metals are known to be major enzyme inhibitors in parasitic helminths (e.g. Goil, 1978;Goil & Harpur, 1979) and have been demonstrated to significantly affect cercarial activity (Cross et al, 2001;Morley et al, 2003a).…”

Section: Discussionmentioning

confidence: 99%

Toxicity of cadmium and zinc mixtures to the decaudized cercarial life span of Diplostomum spathaceum

2005

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The effects of cadmium and zinc mixtures at concentrations ranging from 0.1 to 10,000 microg l(-1) on the life-span of decaudized cercarial bodies (cercariae that have shed their tails) of Diplostomum spathaceum (Trematoda: Diplostomatidae) was investigated. Cercariae were exposed to metal mixtures of equal and unequal concentrations, and a low-dose pre-treatment followed by a high-dose exposure mixtures. Metal mixtures demonstrated variable effects on decaudized cercariae either by increasing or reducing their life-span compared to single metal exposures dependent on concentration and the type of mixed metal treatment. Prolonged exposure to equal metal mixtures at low concentrations (0.1-100 microg l(-1)) resulted in a reduction in the life-span of decaudized cercariae at 0.1 and 100 microg l(-1) in those individuals decaudized during the initial 24 h exposure period compared with those decaudized during the final 24 h period of cercarial survival, whilst in controls there was no significant life-span change between the two time periods. Decaudized cercariae which were exposed to low concentrations (0.1-100 microg l(-1)) of equal metal mixtures were also evaluated for their role as an indicator of larval 'fitness' for migrating through the tissues of their target fish host for those individuals decaudized during the initial 24 h exposure period, and demonstrated only a limited change in their life-span compared to control and single metal exposures. The importance of metal mixtures in parasite establishment in the fish host is discussed.

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“…More recent work has associated metal toxicity to cercarial survival with the impaired utilization of glycogen (Siddall & des Clers, 1994 ;Morley et al 2001. Heavy metals, in particular cadmium, are known to be potent inhibitors of the enzymes involved in carbohydrate metabolism in both parasitic helminths (Murthy & Tayal, 1978 ;Parshad & Guraya, 1978 ;Goil, 1978 ;Goil & Harpur, 1979 ;Gupta & Trivedi, 1986) and other invertebrates (Ortel, 1996). It is therefore apparent from the published literature that there are direct links between cercarial glycogen utilization, survival and infectivity.…”

Section: Introductionmentioning

confidence: 99%

Toxicity of cadmium and zinc to the decaudised cercarial life-span of Diplostomum spathaceum (Trematoda: Diplostomidae)

2003

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The toxicity of cadmium and zinc at concentrations ranging from 0.1 to 10000 microg/l to the life-span of decaudised cercarial bodies (cercariae that have shed their tails) of Diplostomum spathaceum was investigated. The effects of metal exposure at 3 temperatures (12, 20, and 25 degrees C) and 3 levels of water hardness (distilled water, soft water and hard water) were studied. In general, under most experimental conditions increasing metal concentrations reduced the life-span of decaudised cercariae. Increasing water hardness and decreasing water temperature caused an increase in the life-span of both control and metal exposed decaudised cercariae. However, at certain isolated metal concentrations, associated with a specific level of water hardness and temperature, increased survival above controls occurred. Differences in the relative toxicity of cadmium and zinc were dependent on the environmental conditions of exposure. The decaudised cercarial life-span under metal exposure was found to be generally independent of the overall cercarial survival and tail loss in most experimental conditions. Prolonged exposure to cadmium and zinc caused changes in the decaudised cercarial life-span when compared to individuals decaudised during the initial 24 h exposure period to those which were decaudised during the final 24 h period of cercarial survival. The validity of studying the decaudised cercarial life-span as an indicator of 'fitness' of larvae to migrate through the tissues of the target fish host, in terms of glycogen utilization, was assessed for those cercariae decaudised during the initial 24 h exposure period only. A limited reduction in the decaudised cercarial life-span during this period compared to controls was recorded, which may possibly indicate a reduced penetration 'fitness' of cercariae exposed to cadmium and zinc. The importance and relevance of these findings to parasite migration and establishment in the fish host are discussed.

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A comparison of the non-specific acid phosphomonoesterase activity in the larva ofPhocanema decipiens (Nematoda) with that of the muscle of its host the codfish (Gadus morhua)

Cited by 6 publications

References 16 publications

Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems

Determination of the Proportion of Total Soil Extracellular Acid Phosphomonoesterase (E.C. 3.1.3.2) Activity Represented by Roots in the Soil of Different Forest Ecosystems

Toxicity of cadmium and zinc mixtures to the decaudized cercarial life span of Diplostomum spathaceum

Toxicity of cadmium and zinc to the decaudised cercarial life-span of Diplostomum spathaceum (Trematoda: Diplostomidae)

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