Durable materials for Nzi traps were tested in three applied settings based on promising formats that were tested first in Canada. Experiments were conducted at Cape Cod, Massachusetts, United States of America at the site of a major tabanid control programme and at two challenging tropical locations (Sudan and Philippines) where mechanical transmission of trypanosomiasis occurs. At Cape Cod, royal blue–painted plywood Nzi traps caught up to 19 times as many tabanids as greenhead box traps used locally did. Fabric Nzi traps caught up to six times more horseflies, Tabanus spp. (Diptera: Tabanidae), than a box trap did and twice as many horseflies than a popular commercial trap, the Horse Pal® did. A home-dyed phthalogen turquoise cotton trap also performed well. In the Philippines, it caught 2.5 times as many Tabanus as a standard phthalogen blue cotton trap did. Durable phthalogen blue Sunbrella acrylic and Top Notch polyester traps with various types of netting performed well at all locations.