A comparison of the tube forming potentials of early and late endothelial progenitor cells

Formação in vitro de túbulos capilares a partir de células de sangue de cordão umbilical humano com perspectivas para aplicação terapêutica

“…It was shown that these cells not only participate in the induction of paracrine factors -which may stimulate the proliferation of in situ cells -but also in the physical reconstruction of the lesioned area. In another study, Mukai et al [23] confirmed the findings of this study, showing that cultivated EPCs could form blood vessels, unlike from EPCs without cultivation, which would promote angiogenesis through the migration and proliferation of mature ECs, suggesting that these two cell populations would have a different role in in vivo neovascularization.…”

Endothelial Nitric Oxide Synthase as A Marker for Human Endothelial Progenitor Cells

“…Mukai et al (2008) have compared the tube formation potentials of the two types of cells and found that EC-like cells caused the disorganization of pre-existing vessels, whereas EOCs constituted and orchestrated vascular tube formation. And other studies found that these two types of cells might have different roles in neovasculogenesis and mixed transplantation of these cells EC-like cells and EOCs were cultured in growth factor-free medium over a 72-hour period: a. VEGF, b. IL-8, c. G-CSF.…”

Induction of apoptosis in circulating angiogenic cells by microparticles

“…These cells were formerly considered as a subpopulation of endothelial progenitor cells (EPCs) named "early outgrowth EPCs" and are now regarded as a distinct (progenitor) cell type (11). In contrast to "true" late outgrowth EPCs, it is controversial whether CACs differentiate into mature ECs and thus contribute directly to vasculogenesis (12). However, there is robust evidence that CACs are potent circulating regulators of angiogenic responses in ischemic tissues, and they also contribute to the repair of damaged preexisting vessels (13,14).…”