A comparison of thermal adaptation of membrane lipids in psychrophilic and thermophilic bacteria

Russell, Nick J.; Fukunaga, Noriyuki

doi:10.1111/j.1574-6968.1990.tb04093.x

Cited by 217 publications

(58 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A change in the membrane fluidity is often caused by a change in the lipid composition of the membranes to keep an adequate proportion of liquid-crystalline lipid in the membrane and to maintain the bilayer phase (Russell and Fukunaga 1990). Ingram (1976) found that growth of Escherichia coli was inhibited by lipophilic agents, such as alcohols.…”

Section: Discussionmentioning

confidence: 99%

“…To withstand harmful effects of cytotoxic compounds, bacterial cells possess several adaptation mechanisms. Three different mechanisms compensate for the accumulation of toxic hydrophobic compounds in the membrane: (1) keeping an adequate proportion of the liquid-crystalline lipid in the membrane (restoration of membrane fluidity), (2) the maintenance of a proper balance between bilayer-and non-bilayer-promoting phospholipids (keeping membrane integrity) (Bygraves and Russell 1988;Russell and Fukunaga 1990;Weber and De Bont 1996) and (3) extrusion by drug extrusion systems (Bolhuis et al 1994).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adaptation of the food-borne pathogen Bacillus cereus to carvacrol

Ultee¹,

Kets²,

Alberda³

et al. 2000

Archives of Microbiology

254

149

View full text Add to dashboard Cite

Carvacrol, a natural antimicrobial compound present in the essential oil fraction of oregano and thyme, is bactericidal towards Bacillus cereus. A decrease of the sensitivity of B. cereus towards carvacrol was observed after growth in the presence of non-lethal carvacrol concentrations. A decrease of the melting temperature (Tm) of membranes from 20.5 degrees C to 12.6 degrees C was the immediate effect of the addition of carvacrol. Cells adapted to 0.4 mM carvacrol showed a lower membrane fluidity than nonadapted cells. Adaptation of 0.4 mM carvacrol increased the Tm from 20.5 degrees C to 28.3 degrees C. The addition of carvacrol to cell suspensions of adapted B. cereus cells decreased Tm again to 19.5 degrees C, approximately the same value as for the non-adapted cells in the absence of carvacrol. During adaptation, changes in the fatty acid composition were observed. The relative amount of iso-C13:0, C14:0, and iso-C15:0 increased and cis-C16:1 and C18:0 decreased. The head-group composition also changed, two additional phospholipids were formed and one phospholipid was lacking in the adapted cells. It could be concluded that B. cereus adapts to carvacrol when present at non-lethal concentrations in the growth medium by lowering its membrane fluidity by changing the fatty acid and headgroup composition.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adaptation of the food-borne pathogen Bacillus cereus to carvacrol

Ultee¹,

Kets²,

Alberda³

et al. 2000

Archives of Microbiology

254

149

View full text Add to dashboard Cite

show abstract

“…Physiologically, thermotolerance is closely related to the endogenous accumulation of polyols and trehalose in fungi [9-11, 22, 26], and the alteration of cell wall lipid compositions in yeast and bacteria [7,8,23,29]. Such tolerance can be acquired by careful manipulation of the osmotic pressure of culture media [9,21,28] or by supplying materials as substrates for the induction of thermotolerance [3,14,24,25,27].…”

Section: Introductionmentioning

confidence: 99%

Production of thermotolerant entomopathogenic Isaria fumosorosea SFP-198 conidia in corn-corn oil mixture

Roh

2010

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

Low thermotolerance of entomopathogenic fungi is a major impediment to long-term storage and effective application of these biopesticides under seasonal high temperatures. The effects of high temperatures on the viability of an entomopathogenic fungus, Isaria fumosorosea SFP-198 (KCTC 0499BP), produced on different substrates amended with various additives were explored. Ground corn was found to be superior in producing the most thermotolerant conidia compared to yellow soybean, red kidney bean, and rice in a polyethylene bag production system. Using ground corn mixed with corn oil as a substrate resulted in only 7% reduction in germination compared to ground corn alone (67% reduction) after exposure of conidia to 50 degrees C for 2 h. Corn oil as an additive for ground corn was followed by inorganic salts (KCl and NaCl), carbohydrates (sucrose and dextrin), a sugar alcohol (sorbitol), and plant oils (soybean oil and cotton seed oil) in ability to improve conidial thermotolerance. Unsaturated fatty acids, such as linoleic acid and oleic acid, the main components of corn oil, served as effective additives for conidial thermotolerance in a dosage-dependent manner, possibly explaining the improvement by corn oil. This finding suggests that the corn-corn oil mixture can be used to produce highly thermotolerant SFP-198 conidia and provides the relation of unsaturated fatty acids as substrates with conidial thermotolerance.

show abstract

“…The fatty acid pattern of lipids can be characterized by the degree of saturation, which is taken as an important parameter of the fluidity of bacterial membranes (Shinitzky 1984). Many studies show that the fluidity of the lipid bilayer is strongly regulated to offset physical and chemical changes imposed by the environment (Sinensky 1974;Russell and Fukunaga 1990). This homeoviscous adaptation process probably enables vital membrane functions to continue.…”

Section: Introductionmentioning

confidence: 99%

The conversion of cis into trans unsaturated fatty acids in Pseudomonas putita P8: evidence for a role in the regulation of membrane fluidity

Diefenbach

Heipieper

Keweloh

1992

Appl Microbiol Biotechnol

114

View full text Add to dashboard Cite

The physiological significance of trans unsaturated fatty acids, which are constituents of membrane lipids of the phenol-degrading bacterium Pseudomonas putita P8, was studied. The addition of phenol or phenol derivatives to the cells induced the formation of trans unsaturated fatty acids, yielding an overall maximal amount of 41.3°70 of total fatty acids. The inhibition of de-novo lipid synthesis by cerulenin prevented the change in the degree of saturation in the lipids. However, the cells could still respond to phenols with an amplified conversion of cis into trans unsaturated fatty acids, which is apparently a post-synthetic mechanism of isomerization of the double bond. The cis/trans conversion correlated with growth inhibition induced by toxic concentrations of 4-chlorophenol, whereas only growing cells were able to change the degree of saturation. In cells that were protected against phenol by immobilization in calcium alginate, the conversion of cis into trans fatty acids occurred at higher toxin concentrations compared with free ceils. Cells entering the stationary growth phase increased the proportion of saturated to unsaturated fatty acids but maintained a constant trans/cis ratio. P. putida P8 reacted to an increase or decrease in the growth temperature with an appropriate change in the ratio of saturated to unsaturated fatty acids and in ceils inhibited by cerulenin with a change in the trans/cis ratio. This study shows that the physiological role of the cis/trans conversion is probably the regulation of membrane fluidity when the most important mechanism for this, the modification of the degree of saturation, cannot be used by the cells due to inhibition of growth and lipid biosynthesis.

show abstract

A comparison of thermal adaptation of membrane lipids in psychrophilic and thermophilic bacteria

Cited by 217 publications

References 50 publications

Adaptation of the food-borne pathogen Bacillus cereus to carvacrol

Adaptation of the food-borne pathogen Bacillus cereus to carvacrol

Production of thermotolerant entomopathogenic Isaria fumosorosea SFP-198 conidia in corn-corn oil mixture

The conversion of cis into trans unsaturated fatty acids in Pseudomonas putita P8: evidence for a role in the regulation of membrane fluidity

Contact Info

Product

Resources

About