A comparison of three fluorophores for use in an optical biosensor for the measurement of prostate-specific antigen in whole blood

Daniels, P.B.; Fletcher, Janys E.; O’Neill, Paul M.; Stafford, Christopher G.; Bacarese-Hamilton, Timothy; Robinson, G.A.

doi:10.1016/0925-4005(94)01637-w

Cited by 23 publications

(11 citation statements)

References 6 publications

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“…The addition in the immunoreaction mixture of substances acting as bulk fluorescence light blockers is a potential solution to this problem. An improved performance was observed for the fluorescence capillary fill device (Daniels et al, 1995) when the sample matrix was changed from serum to whole blood. In that case, the red cells served as light blockers absorbing the bulk fluorescence.…”

Section: Introductionmentioning

confidence: 82%

Bulk fluorescence light blockers to improve homogeneous detection in capillary-waveguide fluoroimmunosensors

Mastichiadis

Petrou

Christofidis

et al. 2009

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 82%

Bulk fluorescence light blockers to improve homogeneous detection in capillary-waveguide fluoroimmunosensors

Mastichiadis

Petrou

Christofidis

et al. 2009

Biosensors and Bioelectronics

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“…This attempt allows continuous measurements without a separation step. PSA in whole blood was analyzed with good performance characteristics by Daniels et al [78], applying allophycocyanin as fluorophore in an FCFD system. A fiber optic TIRF system was used by Mahoney et al [5] for the measurement of hCG in serum.…”

Section: Optical Systemsmentioning

confidence: 99%

Immunosensor Technology - Principles and Applications. Immunosensor-Technologie - Grundlagen und Anwendungen

Luppa¹

2001

LaboratoriumsMedizin

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Immunosensors are affinity ligand-based biosensor solid-state devices in which the immunochemical reaction is coupled to a transducer. The fundamental basis of all immunosensors is the specificity of the molecular recognition of antigens by antibodies to form a stable complex. This is similar to immunoassny methodology. Immunosensors can be categorized based on the detection principle applied. The main developments are electrochemical, optical, and microgravimetric immunosensors. In contrast to immunoassay, modern transducer technology enables the label-free detection and quantification of the immune complex. The analysis of trace substances in environmental science, pharmaceutical and food industries is a challenge since many of these applications demand a continuous monitoring mode. The use of immunosensors in these applications is most appropriate. Similarly, a series of clinical problems may be solved by continuous monitoring of certain analytes. Hence, clinical chemists should take advantage of immunosensors in clinical diagnostics. Therefore, the aim of this review is to portray the recent developments in the immunosensor field and their potential impacts in clinical diagnostics.

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“…Optical fiber immunosensor sensitivity has been reported to be in the range 10 -1 -10 -14 M [4,86]. Compared with immunoassays using an enzyme label, the fluorescent dye is much more stable and guarantees a shelf-life of several years.…”

Section: Immunoptodementioning

confidence: 99%

Immunosensors for Diagnostics

2001

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Immunosensors can play an important role in the improvement of public health by providing applications for which rapid detection, high sensitivity, and specificity are important, in areas such as clinical chemistry, food quality, and environmental monitoring. The standard immunoassay techniques are the basis for the development of improved automated devices, exploring the accumulated know how of the implementation of indirect detection methods during the last 40 years. Today's systems achieve high accuracy, specificity, and sample throughput. Disposable immunotests (qualitative and quantitative), based on colored detection of an immunoreaction, are continuously expanding its applications for diagnosis of diseases, drug detection, and food quality control.Associated to the increasing demand of immunoapplications and with the new advances produced in the last decades in fields like biochemistry, biotechnology, electronics, and microfabrication, new transduction devices have been developed and applied to monitor immunoreactions. Optimization of the biochemical characteristics of the binding molecules, improved surface stability of the transducer interfaces, and reliable microsystems and integrated devices are the challenge for the next years of development. An overview of the immunoassay techniques used today in standard diagnosis will be presented, including a brief description of the different immunosensor transducers and some examples of the present developments and commercial devices.

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A comparison of three fluorophores for use in an optical biosensor for the measurement of prostate-specific antigen in whole blood

Cited by 23 publications

References 6 publications

Bulk fluorescence light blockers to improve homogeneous detection in capillary-waveguide fluoroimmunosensors

Bulk fluorescence light blockers to improve homogeneous detection in capillary-waveguide fluoroimmunosensors

Immunosensor Technology - Principles and Applications. Immunosensor-Technologie - Grundlagen und Anwendungen

Immunosensors for Diagnostics

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