A comparison of three methods of measuring 99mTc-DTPA lung clearance and their repeatability

Morrison, Douglas; Skwarski, K; Am, Millar; Adams, W. E.; MacNee, William

doi:10.1183/09031936.98.11051141

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…Lung permeability measurements in this study showed little variation within the subject across three different visits, across the three groups, attesting to the repeatability of this method. In agreement with our findings, Morrison et al demonstrated that lung permeability measurements showed minimal variability in two separate study visits (29).…”

Section: Discussionsupporting

confidence: 93%

Clinical Evaluation of Alveolar Permeability as a Biomarker of Potential Harm

Makena,

Baxter-Write,

Jones

et al. 2023

Preprint

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The lung epithelium regulates gas exchange and is the first line of defense against airborne toxicants and microbial infections. Disruption of the integrity of lung epithelium results in altered permeability and severely impacts normal lung functions. To develop biomarkers of potential harm (BoPH) for assessing adverse health effects of cigarette smoking, we have evaluated lung barrier function. In a single-center, ambulatory, clinical study we measured lung permeability, as the half-life (T1/2) of inhaled 99mTC-DTPA, in 17 subjects consisting of 6 Smokers (SMK), 5 Moist Snuff Consumers (MSC), and 6 Non-Tobacco Consumers (NTC) at three weekly test visits. Smokers, relative to MSC and NTC, exhibited significantly faster clearance of the inhaled probe (shorter T1/2), indicating increased lung permeability. NTC and MSC had comparable clearance times of the inhaled probe (longer T1/2). Least squares regression modeling showed that the variability of the test was minimal and was not significant. Prediction profiling showed that the repeatability of the lung permeability test was robust and was able to differentiate the tobacco product use groups in this study. Thus, altered lung barrier function may serve as a BoPH for assessing the effects of tobacco use.

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Section: Discussionsupporting

confidence: 93%

Clinical Evaluation of Alveolar Permeability as a Biomarker of Potential Harm

Makena,

Baxter-Write,

Jones

et al. 2023

Preprint

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“…Counts at peak and 7 min after the peak were taken for calculation. The reason behind the restriction to 7 min after reaching the peak was to minimize the influence of background radioactivity [18]. A least-square fit was carried out for regression analysis.…”

Section: Methodsmentioning

confidence: 99%

Lung epithelial permeability and inhaled furosemide: added dimensions in asthmatics

Bhure¹,

Bhatt²,

Mistry³

et al. 2009

Ann Nucl Med

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Baseline lung epithelial permeability was altered in smokers and asthmatics compared to the controls. Furosemide was effective only in asthmatics in reverting the permeability almost back to the normal range. Inhaled furosemide was effective even in moderate and severe asthmatics. Furosemide has multiple mechanisms of action. It possibly acts at bronchial level in view of the pathology in asthmatics lying in the airways.

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“…We have previously demonstrated the reproducibility of the foregoing technique (14). In the case of three chronic smokers, thin layer chromatography (TLC) of residual 99m Tc-DTPA in the nebulizer and of 99m Tc-DTPA in urine demonstrated 0% and Ͻ 1.7% dissociation, respectively, into DTPA and free 99m Tc-pertechnetate (15).…”

Section: M Tc-dtpa Lung Clearancementioning

confidence: 97%

Epithelial Permeability, Inflammation, and Oxidant Stress in the Air Spaces of Smokers

Morrison

Rahman

Lannan

et al. 1999

Am J Respir Crit Care Med

255

167

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The mechanism responsible for the increased air-space permeability in cigarette smokers is unknown. The aim of this study was to assess the acute and chronic effects of cigarette smoking on epithelial permeability, inflammation, and oxidant stress in the air spaces of smokers. Fourteen cigarette smokers underwent 99mTc-diethylenetriamine pentaacetic acid (99mTc-DTPA) lung scans after abstaining from smoking for 12 h (chronic smoking) and 1 h after smoking two cigarettes (acute smoking). Each smoker also underwent bronchoscopy and bronchoalveolar lavage (BAL) after either chronic (n = 8) or acute smoking (n = 7). Seven nonsmokers also underwent bronchoscopy and BAL. The time to 50% clearance of 99mTc-DTPA (t50) after chronic smoking was 16.7 +/- 1. 3 min (mean +/- SE), and was further reduced after acute smoking to 14.8 +/- 1.0 min (p < 0.01). Neutrophil numbers were increased in bronchoalveolar lavage fluid (BALF) in the acute smoking group as compared with the nonsmokers (p < 0.05). Superoxide release from mixed BAL leukocytes was increased after chronic (p < 0.01) and acute (p < 0.001) smoking, as were thiobarbituric acid-reactive species (TBARS), providing evidence of lipid peroxidation in plasma (chronic, p < 0.05; acute, p < 0.05). Trolox equivalent antioxidant capacity (TEAC) was reduced in plasma (p < 0.001) and increased in BALF (p < 0.05) in both smoking groups. The study therefore showed an acute increase in epithelial permeability and an increase in the number of neutrophils in the air spaces of cigarette smokers concomitant with evidence of increased oxidant stress.

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A comparison of three methods of measuring 99mTc-DTPA lung clearance and their repeatability

Cited by 6 publications

References 23 publications

Clinical Evaluation of Alveolar Permeability as a Biomarker of Potential Harm

Clinical Evaluation of Alveolar Permeability as a Biomarker of Potential Harm

Lung epithelial permeability and inhaled furosemide: added dimensions in asthmatics

Epithelial Permeability, Inflammation, and Oxidant Stress in the Air Spaces of Smokers

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