A Comparison of Three Potyviruses by Direct Hybridization Analysis

Reddick, B. B.; Barnett, O. W.

doi:10.1094/phyto-73-1506

Cited by 42 publications

(23 citation statements)

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“…Demski and were the original isolates described by him [2], The peanut mild mottle virus (PStV-Mild Mottle) isolate was that de scribed by Xu et al [3]. These isolates were propagated in white lupin and isolated as described [2], B1CMV-Type (Florida isolate), SbMV (Wisconsin isolate), ClYW -Pratt [13], the Scott strain of bean yellow mosaic virus (BYMV-Scott), and tobacco etch virus (TEV; ori ginally from R.W. Fulton, Wisconsin) were purified according to method two of Reddick and Barnett [13].…”

Section: Methodsmentioning

confidence: 99%

Coat Protein Properties Suggest that Azuki Bean Mosaic Virus, Blackeye Cowpea Mosaic Virus, Peanut Stripe Virus, and Three Isolates from Soybean Are All Strains of the Same Potyvirus

et al. 1992

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The interrelationship of a number of potyviruses infecting legumes has been investigated by comparing molecular properties of their coat proteins. Comparison of the coat proteins by the techniques of amino acid analysis and PAGE was inadequate to distinguish strains from distinct potyviruses. However, high-performance liquid chromatographic peptide profiles of tryptic digests of coat proteins of these legume-infecting potyviruses enabled such assignments to be made. These data indicate that amino acid sequences of coat proteins of azuki bean mosaic virus, the Type and W strains of blackeye cowpea mosaic virus, three isolates (74, PM, PN) of a potyvirus obtained from soybean in Taiwan, and the Blotch and Mild Mottle strains of peanut stripe virus (PStV) may be very similar to the known sequence of PStV Stripe coat protein. In contrast, peptide profiles of coat proteins from soybean mosaic virus, clover yellow vein virus, bean yellow mosaic virus, potato virus Y, and tobacco etch virus were dissimilar to each other and to the profile of PStV Stripe, suggesting that their coat protein sequences were also quite different. Based on observations of the coat protein structure of many potyviruses, the results suggest that the potyvirus isolates with similar coat proteins are strains of the same potyvirus.

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Section: Methodsmentioning

confidence: 99%

Coat Protein Properties Suggest that Azuki Bean Mosaic Virus, Blackeye Cowpea Mosaic Virus, Peanut Stripe Virus, and Three Isolates from Soybean Are All Strains of the Same Potyvirus

et al. 1992

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“…In legumes, BYMV is transmitted by several species of aphids in a non-persistent manner, and also experimentally in sap of infected plants (Bos 1970 a, Tapio 1970, Cockbain 1983 1984, Jayasena and Randles 1985, Bays and Demski 1986, Winter and Nienhaus 1989, Provvidenti 1991. Many strains of BYMV have been reported which may be distinguished by host range, aphid transmission, serology or RNA sequence heterology (Bos 1970 a, Jones and Diachun 1977, Reddick and Barnett 1983, Schmidt and Zobywalski 1984, Herrera and Sepulveda 1986, Barnett et al 1987, Scott et al 1989, Hopps and Mclaughlin 1990. The pea strain of BYMV, formerly considered a distinct virus from the bean strain of BYMV and called pea mosaic virus (PMV), causes bright yellow mosaic symptoms, whereas the bean strain of BYMV causes green mosaic symptoms in pea leaves (Bos 1970b, Tapio 1970).…”

Section: Introductionmentioning

confidence: 99%

Three strains of bean yellow mosaic virus: symptoms and accumulation in eight pea cultivars (Pisum sativum L.)

Valkonen

1993

AFSci

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Helsinki, Finland.) A pea mosaic strain and a bean strain of bean yellow mosaic virus (BYMV) were isolated from naturally infected pea and broad bean plants and named BYMV-Ps and BYMV-Vf, respectively. A third strain of BYMV isolated from Gladiolus (BYMV-G) was obtained from Denmark which was distinguished from the two above strains serologically and by its symptoms in test plants.BYMV-Ps and BYMV-Vfcaused yellow mosaic symptoms and green mosaic symptoms, respectively, in eight pea cultivars tested, but the concentration of BYMV varied among the cultivars. BYMV-G caused mild mosaic or vein clearing in peas. A need to improve resistance to BYMV in the Finnish pea varieties was recognized.

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“…Properties of potyviruses that have been used to differentiate individual viruses and their strains have included host range, cross-protection, morphology of cytoplasmic inclusions, amino acid composition, serology and cDNA hybridization (Bos, 1970;Jones & Diachun, 1977;Edwardson, 1974;Moghal & Francki, 1976;Randles et al, 1980;Abu-Samah & Randles, 1981 ;Reddick & Barnett, 1983). Among these, serological properties appear to offer the most practical method at present for establishing the identity of a new isolate.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of Potyviruses and Their Strains by High Performance Liquid Chromatographic Peptide Profiling of Coat Proteins

Shukla

McKern

Gough

et al. 1988

Journal of General Virology

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SUMMARYComparison by HPLC of tryptic digests of coat proteins from six biologically and serologically distinct potyviruses, namely bean yellow mosaic virus, Johnson grass mosaic virus, passion-fruit woodiness virus (PWV), potato virus Y (PVY), sugarcane mosaic virus (SCMV) and water-melon mosaic virus II, demonstrated that each potyvirus can be distinguished from the others. HPLC of tryptic peptides from coat proteins of four strains of PVY, two strains of PWV and three strains of SCMV showed that peptide patterns of strains from the same potyvirus were very similar. These findings were supported by amino-terminal amino acid sequence analysis of the peptides. The use of enzymes from different sources and variation in the temperature (35 °C to 40 °C) and time (16 to 20 h) of digestion caused small variations in the profiles but did not change the main features of the peptide patterns of each potyvirus. The results suggest that HPLC profiles of tryptic digests of the coat proteins of potyviruses could be useful criteria for the identification and classification of potyviruses.

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A Comparison of Three Potyviruses by Direct Hybridization Analysis

Cited by 42 publications

References 0 publications

Coat Protein Properties Suggest that Azuki Bean Mosaic Virus, Blackeye Cowpea Mosaic Virus, Peanut Stripe Virus, and Three Isolates from Soybean Are All Strains of the Same Potyvirus

Coat Protein Properties Suggest that Azuki Bean Mosaic Virus, Blackeye Cowpea Mosaic Virus, Peanut Stripe Virus, and Three Isolates from Soybean Are All Strains of the Same Potyvirus

Three strains of bean yellow mosaic virus: symptoms and accumulation in eight pea cultivars (Pisum sativum L.)

Differentiation of Potyviruses and Their Strains by High Performance Liquid Chromatographic Peptide Profiling of Coat Proteins

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