A Comparison of Two <i>Nicotiana attenuata</i> Accessions Reveals Large Differences in Signaling Induced by Oral Secretions of the Specialist Herbivore <i>Manduca sexta</i>

Wu, Jianqiang; Hettenhausen, Christian; Schuman, Meredith C.; Baldwin, Ian Thomas

doi:10.1104/pp.107.114785

Cited by 70 publications

(68 citation statements)

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“…S5). Similar variation in the magnitude of Spodoptera OS-induced VOC emission in diverse maize cultivars and defense signaling in different tobacco populations has been previously described (36,37).…”

Section: Maize Displays Intraspecific Differences In Response To Elicmentioning

confidence: 75%

Phytohormone-based activity mapping of insect herbivore-produced elicitors

Schmelz

Engelberth

Alborn

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

233

225

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In response to insect attack, many plants exhibit dynamic biochemical changes, resulting in the induced production of direct and indirect defenses. Elicitors present in herbivore oral secretions are believed to positively regulate many inducible plant defenses; however, little is known about the specificity of elicitor recognition in plants. To investigate the phylogenic distribution of elicitor activity, we tested representatives from three different elicitor classes on the time course of defense-related phytohormone production, including ethylene (E), jasmonic acid (JA), and salicylic acid, in a range of plant species spanning angiosperm diversity. All families examined responded to at least one elicitor class with significant increases in E and JA production within 1 to 2 h after treatment, yet elicitation activity among species was highly idiosyncratic. The fatty-acid amino acid conjugate volicitin exhibited the widest range of phytohormone and volatile inducing activity, which spanned maize (Zea mays), soybean (Glycine max), and eggplant (Solanum melongena). In contrast, the activity of inceptinrelated peptides, originally described in cowpea (Vigna unguiculata), was limited even within the Fabaceae. Similarly, caeliferin A16:0, a disulfooxy fatty acid from grasshoppers, was the only elicitor with demonstrable activity in Arabidopsis thaliana. Although precise mechanisms remain unknown, the unpredictable nature of elicitor activity between plant species supports the existence of specific receptor-ligand interactions mediating recognition. Despite the lack of an ideal plant model for studying the action of numerous elicitors, E and JA exist as highly conserved and readily quantifiable markers for future discoveries in this field.ethylene ͉ insect elicitor ͉ jasmonic acid ͉ plant defense ͉ volatile organic compound

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Section: Maize Displays Intraspecific Differences In Response To Elicmentioning

confidence: 75%

Phytohormone-based activity mapping of insect herbivore-produced elicitors

Schmelz

Engelberth

Alborn

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

233

225

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“…As previously shown for N. attenuata volatile emissions (46), we concluded from this analysis that natural variations in levels of herbivore-induced metabolites only partly overlap with upstream variations in jasmonate accumulation. This result underscores that complex signaling interactions involving not only jasmonates but also other phytohormones and signals vary across accessions (47). A fundamental aspect of this explorative analysis is that it sheds light on many interesting unknown m/z signals.…”

Section: Natural Variation In Herbivore-induced Levels Of Known and Umentioning

confidence: 95%

Navigating natural variation in herbivory-induced secondary metabolism in coyote tobacco populations using MS/MS structural analysis

Baldwin

Gaquerel

2015

Proc. Natl. Acad. Sci. U.S.A.

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Natural variation can be extremely useful in unraveling the determinants of phenotypic trait evolution but has rarely been analyzed with unbiased metabolic profiling to understand how its effects are organized at the level of biochemical pathways. Native populations of Nicotiana attenuata, a wild tobacco species, have been shown to be highly genetically diverse for traits important for their interactions with insects. To resolve the chemodiversity existing in these populations, we developed a metabolomics and computational pipeline to annotate leaf metabolic responses to Manduca sexta herbivory. We selected seeds from 43 accessions of different populations from the southwestern United Statesincluding the well-characterized Utah 30th generation inbred accession-and grew 183 plants in the glasshouse for standardized herbivory elicitation. Metabolic profiles were generated from elicited leaves of each plant using a high-throughput ultra HPLC (UHPLC)-quadrupole TOFMS (qTOFMS) method, processed to systematically infer covariation patterns among biochemically related metabolites, as well as unknown ones, and finally assembled to map natural variation. Navigating this map revealed metabolic branch-specific variations that surprisingly only partly overlapped with jasmonate accumulation polymorphisms and deviated from canonical jasmonate signaling. Fragmentation analysis via indiscriminant tandem mass spectrometry (idMS/MS) was conducted with 10 accessions that spanned a large proportion of the variance found in the complete accession dataset, and compound spectra were computationally assembled into spectral similarity networks. The biological information captured by this networking approach facilitates the mining of the mass spectral data of unknowns with high natural variation, as demonstrated by the annotation of a strongly herbivory-inducible phenolic derivative, and can guide pathway analysis.plant-insect interactions | metabolomics | mass spectrometry | natural variation E lucidating the structure of metabolites underlying complex traits and the factors that maintain their variation in natural populations are important challenges in plant ecological studies (1). Many studies have notably shown that stress-responsive pathways that produce secondary metabolites are sporadically found across different plant taxa with extensive diversification (2). This important diversification suggests that particular metabolic systems have been recruited through natural selection when the set of compounds that they produce address specific ecological needs. Interactions with insects are important selection pressures that have sculpted plant metabolism, and many plant metabolites protect against herbivore attack and physical damage (3-5). The timely production of particular secondary metabolites in response to insect attack benefits plants by decreasing the costs of constitutive metabolite production. Trade-offs between defense metabolite productions and the intrinsic growth-related functions of central metabolic pathways likely prov...

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“…In their Update, Mithö fer and Boland (2008) discuss the early signaling events at the plant-herbivore interface. Wu et al (2008) show that there can be significant within-species differences in these early signaling responses. Frost et al (2008) describe molecular and ecological aspects of defense ''priming,'' which has become an important area of research.…”

mentioning

confidence: 99%